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:: Volume 4, Number 48 (12-2013) ::
JMP 2013, 4(48): 82-90 Back to browse issues page
Improvement of Artemisinin Production in Hairy Roots Culture of Artemisia annua L. by Staphylococcus aureus
E Bandeh-Ali1, M Keyhanfar *2, G Asghari3
1- M.Sc. Student Faculty of Advanced Sciences and Technologies, University of Isfahan
2- Ph.D. Faculty of Advanced Sciences and Technologies, University of Isfahan , m.keyhanfar@ast.ui.ac.ir
3- Ph.D. School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences
Abstract:   (2750 Views)
Background: Artemisinin is an important plant secondary metabolite with anti-malaria, anti-viral and anti-cancer properties. In recent years many efforts have been made to improve artemisinin production through plant tissue culture (such as hairy roots). Objective: In this study, the effects of Stephylococcus aureus on artemisinin production in hairy roots of Artemisia annua were investigated. Methods: Agrobacterium rhizogenes strains A7 and Ar318 were used for the root induction. Two explants types were prepared, the first was leaf explants cut from both side (explant 1) and the second was, stems which cut from node (explant 2). The bacterial suspensions (A7 and Ar318) were inoculated at the wounding site of explants 1 and node explants 2. Transgenic nature of hairy roots was confirmed by amplification of rolB gene in polymerase chain reaction (PCR). Gas chromatography (GC) was conducted to determine artemisinin production. Results: About 5 to 10 days after inoculation by A7, hairy roots were appeared at the wounding sites of explants 2. Strain Ar318 could not induce any hairy roots. Also, after treatment with Agrobacterium suspensions, hairy roots were not induced in the explants 1 and explants were necrotic. The artemisinin content in the hairy roots treated with S. aureus suspension was 0.063, 0.133, 0.046 and 0.043 mg/g DW, respectively. Conclusion: Results show that various factors such as type of explants and Agrobacterium strains were effective in hairy roots induction. It seems that Stephylococcus aureus is stimulating the production of artemisinin in hairy roots of Artemisia annua.
Keywords: Artemisia annua L., Agrobacterium rhizogenes, Artemisinin, Hairy roots, Stephylococcus aureus
Full-Text [PDF 305 kb]   (1004 Downloads)    
Type of Study: Research | Subject: Medical Biotechnology
Received: 2014/09/30 | Accepted: 2014/09/30 | Published: 2014/09/30
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Bandeh-Ali E, Keyhanfar M, Asghari G. Improvement of Artemisinin Production in Hairy Roots Culture of Artemisia annua L. by Staphylococcus aureus. JMP. 2013; 4 (48) :82-90
URL: http://jmp.ir/article-1-63-en.html
Volume 4, Number 48 (12-2013) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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