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2- Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran ,
3- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
4- Department of Medicinal Chemistry and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
Background: The seeds of Trigonella foenum-graecum L. (Fenugreek) are used in Iranian traditional medicine as blood sugar lowering agent. According to last investigations, hypoglycemic property is related to the amino acids of the plant specially 4-hydroxyisoleucine.
Objective: In this research, identification and quantitative determination of 4-hydroxyisoleucine in Fenugreek seeds from Shahreza, central part of Iran, has been investigated.
Methods: Powdered seeds of the plant were defatted and then extracted by using ethanol 50%. In order to isolation of 4-hydroxyisoleucine from the extract, ion exchange chromatography was used and the amino acid content was determined by using high performance liquid chromatography technique.
Results: The results showed the presence of 0.4% 4-hydroxyisoleucine in the plant seeds which is considerable amount of the amino acid in the plant collected from Iran.
Received: 2009/12/8 | Accepted: 2010/02/20 | Published: 2010/03/19
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