year 11, Issue 41 And S8 (supplement 8 2012)                   J. Med. Plants 2012, 11(41 And S8): 208-217 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseinzadeh A, Mohajerfar T, Akhondzadeh Basti A, Khanjari A, Gandomi Nasrabadi H, Misaghi A et al . Determination of Minimum Inhibitory Concentration (MIC) of Zataria multiflora Boiss. Essential Oil and Lysozim on E. coli O157: H7. J. Med. Plants 2012; 11 (41) :208-217
1- Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences
2- Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran
3- Faculty of Veterinary Medicine, University of Tehran ,
4- Faculty of Veterinary Medicine, University of Tehran
Abstract:   (7660 Views)

Background: In order to reduce or eliminate foodborne pathogens or food spoilage agents many research have been done to find natural preservatives.

Objective: This study was carried out to determine minimum inhibitory concentration (MIC) of lysozyme and zataria multiflora Boiss. essential oil on E. coli O157: H7 and their sub inhibitory concentration effect on bacterial growth curve.

Methods: In this study different concentrations of lysozyme (0. 125, 250, 500 and 1000 µg/ml) and zataria multiflora Boiss. essential oil (0, 0.005, 0.01, 0.02, 0.04 and 0.08%) were used alone and in combination on BHI broth to determine MIC of zataria multiflora Boiss. essential oil and lysozyme with macro dilution and micro dilution methods and effect of sub inhibitory concentrations of them on bacterial growth curve of E. coli O157: H7.

Results: MIC of essential oil in two methods was achieved 0.04% whereas lysozyme in 1000 µg/ml concentration was not able to reduce bacterial growth. Furthermore combination results revealed that high concentration of lysozyme was not able to reduce calculated amount of MIC of essential oil. Sub inhibitory concentration effect of EO and lysozyme showed that their combination result in the increase of lag phase and reduction of bacterial growth.

Conclusion: utilization of EO and lysozyme together did not decrease MIC but combination of them increase lag phase.
Full-Text [PDF 514 kb]   (3587 Downloads)    
Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2010/10/25 | Accepted: 2011/06/11 | Published: 2012/04/18

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb