Background : Rosa damascena Mill. is one of the most important medicinal and aromatic plants mountainous areas Kashan . This plant have different genotypes that they are very various in size and colour of flowers, number of petals, amount of essential oil and components.

 Objective : Extract ion and determine of essential oil combination of three genotypes from Rose of Kashan area.

 Methods : The flowers of thes e three genophytes fully double link, white and yellow was collected from mountainous areas Kashan. The essential oil, by hydrodistillation using an all-glass Clevenger-type apparatus. The analysis of the oils were performed by using GC and GC-MS methods.

 Results : Yields of essential oil of fully double link genotype was 0.025% (v/w) and 39 components was identification. The major components of this genotype were citronelool(%34.7), nonadecane (%14.5) and heneicosane (%10.3). Yields of the essential oil of white genotype was 0.035% (v/w) and 21 components was identification, that major components were found as c itronellol (%53.61), nonadecane (%17.75) and geraniol (%12.59) . Yields of the essential oil of yellow genotype was 0.7% (v/w) and 29 components was identification, that major components were found a s heneicosane (%32), nonadecane (%30) and 9 -nonadecene (%10.5) .