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:: Volume 4, Number 29 (3-2009) ::
JMP 2009, 4(29): 156-163 Back to browse issues page
Propagation of Ginkgo biloba L. Through Tissue Culture of Various Plant Parts
M Tolyat *1, M Abdoli2, M Ghorbani Moshgin3, F Khalighi-Sigaroodi4, M Omidi5
1- (M.Sc.) 1- Department of Biotechnology Institute of Medical Plants, ACECR, Karaj, Iran , Toliat@acecr.ac.ir
2- (M.Sc., Ph.D. student) 1- Department of Biotechnology Institute of Medical Plants, ACECR, Karaj, Iran
3- (M.Sc.) 3- Iranian Academic Center for Education, Culture and Research, Semnan, Iran
4- (Pharm.D., Ph.D.) 4- Department of Pharmacognosy & Pharmaceutics, Institute of Medicinal Plants, ACECR, Karaj, Iran
5- (Ph.D.) 5- Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Tehran, Iran
Abstract:   (2906 Views)
Background: Ginkgo biloba, often referred to as a living fossil, is the oldest living tree specie on earth and is well known for its resistance to adverse growing conditions as well as its ornamental beauty. Ginkgo has been used as a nutritional support for mental alertness, enhanced circulatory and blood vessel health. The beneficial effects are due to the presence of ginkgolides and bilobalide that represent together with the flavonoids the active constituents. Objective: Propagation of Ginkgo biloba to determine the best explants, medium, kind of hormone on regeneration through tissue culture of various plant parts Methods: In this study factorial experiments were conducted to determine the best explants, medium, kind of hormone on regeneration, the percentage of callus and the weight of wet callus. The response of leaf, petioles and meristem explants on MS, B5, WPM mediums to six hormones were investigated. Every experiment has 3 replications. Every replication for each treatment had 6 samples containing 7 explants. After sterilizing explants, they exposed to the treatments in mediums of %7 Agar (5.7 pH) for 6 weeks. In these experiments only callus was produced therefore it seems that Ginkgo plant is recalcitrant. Results: These results showed that shoot tip was the best explant and interaction of epical meristem and MS medium had the best effect on the production of callus. 1 mg/l NAA plus 0.5 mg BAP was the best hormonal combination. Petiole explant gave the least response.
Keywords: Ginkgo biloba L., Tissue culture, Epical meristem callus, Callus, Plant hormones
Full-Text [PDF 177 kb]   (767 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2014/11/10 | Accepted: 2014/11/10 | Published: 2014/11/10
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Tolyat M, Abdoli M, Ghorbani Moshgin M, Khalighi-Sigaroodi F, Omidi M. Propagation of Ginkgo biloba L. Through Tissue Culture of Various Plant Parts. JMP. 2009; 4 (29) :156-163
URL: http://jmp.ir/article-1-400-en.html
Volume 4, Number 29 (3-2009) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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