year 25, Issue 98 (8-2026)
J. Med. Plants 2026, 25(98): 65-77 |
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Research code: 3400944
Ethics code: IR.MUI.RESEARCH.REC.1400.541
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Izadi M S, Karami Z, Yegdaneh A, Sadraei H. Effects of essential oil and hydroalcoholic extract of Dracocephalum kotschyi on blood pressure in rats. J. Med. Plants 2026; 25 (98) :65-77
URL: http://jmp.ir/article-1-3826-en.html
URL: http://jmp.ir/article-1-3826-en.html
1- Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2- Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
4- Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran ,sadraei@pharm.mui.ac.ir
2- Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
4- Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran ,
Abstract: (15 Views)
Background: Dracocephalum kotschyi is a medicinal plant with well-documented anti-spasmodic and anti-inflammatory properties. However, its potential effects on blood pressure have not been previously investigated. Objective: This study aimed to evaluate the pharmacological effects of D. kotschyi essential oils and its hydroalcoholic extract on blood pressure in rats. Methods: Male Wistar rats were divided into four groups (n = 6/group): vehicle, Nifedipine, D. kotschyi hydroalcoholic extract, and D. kotschyi essential oil. The hydroalcoholic extract and essential oil were prepared by maceration and hydrodistillation, respectively. Under anesthesia, the jugular vein and carotid artery were cannulated, and the carotid cannula was connected to a pressure transducer for continuous blood pressure recording. The effects of intravenous administration of the vehicle, Nifedipine (50-500 µg/kg), the hydroalcoholic extract (20-200 mg/kg), and the essential oil (2-20 mg/kg) on blood pressure were assessed. Results: Intravenous administration of D. kotschyi essential oil significantly reduced blood pressure at 8 mg/kg (P < 0.05) and at 12 and 20 mg/kg (P < 0.01) compared with the vehicle-treated group. In contrast, the hydroalcoholic extract did not significantly affect blood pressure at any of the tested dose. As expected, Nifedipine significantly reduced blood pressure compared with the vehicle-treated group (P < 0.01). Conclusion: The D. kotschyi essential oil contains vasoactive constituents capable of lowering blood pressure. Conversely, the inactivity of the hydroalcoholic extract suggests that its volatile hypotensive constituents were largely lost during rotary evaporation.
Type of Study: Research |
Subject:
Pharmacology & Toxicology
Received: 2025/01/20 | Accepted: 2026/08/18 | Published: 2026/08/29
Received: 2025/01/20 | Accepted: 2026/08/18 | Published: 2026/08/29
References
1. Organization WH. Global report on hypertension: the race against a silent killer. 2023: World Health Organization.
2. Mavrogeni S, Piaditis G, Bacopoulou F and Chrousos GP. Cardiac remodeling in hypertension: clinical impact on brain, heart, and kidney function. Horm. Metab. Res. 2022; 54(05): 273-279. [DOI:10.1055/a-1793-6134]
3. Schutte AE, Kollias A and Stergiou GS. Blood pressure and its variability: classic and novel measurement techniques. Nat. Rev. Cardiol. 2022; 19(10): 643-654. [DOI:10.1038/s41569-022-00690-0]
4. Gallo G, Volpe M and Savoia C. Endothelial dysfunction in hypertension: current concepts and clinical implications. 2022; 8: 798958. [DOI:10.3389/fmed.2021.798958]
5. Ahmad H, Khan H, Haque Sh, Ahmad Sh, Srivastava N and Khan A. Angiotensin-converting enzyme and hypertension: a systemic analysis of various ACE inhibitors, their side effects, and bioactive peptides as a putative therapy for hypertension. 2023; 7890188. [DOI:10.1155/2023/7890188]
6. Carnovale C, Perrotta C, Baldelli S, Cattaneo D, Montrasio C, Barbieri SS, Pompilio G, Vantaggiato Ch, Clementi E and Pozzi M. Antihypertensive drugs and brain function: mechanisms underlying therapeutically beneficial and harmful neuropsychiatric effects. 2023; 119(3): 647-667. [DOI:10.1093/cvr/cvac110]
7. Reeve E, Jordan V, Thompson W, Sawan M, Todd A, Gammie TM, Hopper I, Hilmer SN and Gnjidic D. Withdrawal of antihypertensive drugs in older people. 2020; 6(6): CD012572. [DOI:10.1002/14651858.CD012572.pub2]
8. Nyulas K-I, Simon-Szabó Z, Pál S, Fodor M-A, Dénes L, Cseh MJ, Barabás-Hajdu E, Csipor B, Szakács J, Preg Z, Germán-Salló M and Nemes-Nagy E. Cardiovascular effects of herbal products and their interaction with antihypertensive drugs-Comprehensive review. IJMS. 2024; 25(12): 6388. [DOI:10.3390/ijms25126388]
9. Verma T, Sinha M, Bansal N, Yadav SR, Shah K and Chauhan NS. Plants used as antihypertensive. Nat. Prod. Bioprospect. 2021; 11(2): 155-184. [DOI:10.1007/s13659-020-00281-x]
10. Tsai H-Y, Chuang H-J, Liao W-H, Wang Y-J, Li P-H, Wang W-T, Liao Sh-Ch, Yeh Ch-F, Chen P-R, Lai T-S, Lin H-J, Cheng Sh-Y, Chen W-J, Lin Y-H and Chang Y-Y. Lifestyle modifications and non-pharmacological management in elderly hypertension. 2025; 124(Supp. 1): S32-S41. [DOI:10.1016/j.jfma.2024.10.022]
11. Di Cagno A, Giovanni Fiorilli G, Buonsenso A, Martino GD, Centorbi M, Angiolillo A, Calcagno G, Komici K and Costanzo AD. Long-term physical activity effectively reduces the consumption of antihypertensive drugs: a randomized controlled trial. 2023; 10(7): 285. [DOI:10.3390/jcdd10070285]
12. Kalantar K, Gholijani N, Mousaei N, Yazdani M and Amirghofran Z. Investigation of Dracocephalum kotschyi plant extract on the effective inflammatory transcription factors and mediators in activated macrophages. 2018; 17(1): 39-49. [DOI:10.2174/1871523017666180608081656]
13. Sadraei H, Ghanadian M, Asghari GhR and Sekhavati N. Antispasmodic activity of apigenin and luteolin, two components of Dracocephalum kotschyi extract, on rat ileum contractions. JHP. 2018; 7(2): 100-105. [DOI:10.15171/jhp.2018.17]
14. Sadraei H, Ghanadian SM and Moazeni S. Inhibitory effect of hydroalcoholic and flavonoids extracts of Dracocephalum kotschyi, and its components luteolin, apigenin and apigenin-4'-galactoside on intestinal transit in mice. JHP. 2019; 8(1): 8-13. [DOI:10.15171/jhp.2019.02]
15. Sadraei H, Dastanian M, and Yegdaneh A. Antispasmodic effects of hydroalcoholic, aqueous, chloroform, and ethyl acetate extracts of Zaringiah on rabbit ileum smooth muscle contractions. JHP. 2022; 11(4): 522-528. [DOI:10.34172/jhp.2022.60]
16. Hosseini-Sharifabad A, Sadraei H, Hashemnia M, Sajjadi SE and Mirdamadi Z. Effect of hydroalcoholic and aqueous extracts of Dracocephalum kotschyi on bleomycin induced pulmonary fibrosis. JHP. 2021; 10(2): 209-217. [DOI:10.34172/jhp.2021.23]
17. Sadraei H, Asghari GhR, Khanabadi M and Minaiyan M. Anti-inflammatory effect of apigenin and hydroalcoholic extract of Dracocephalum kotschyi on acetic acid-induced colitis in rats. Res. Pharm. Sci. 2017; 12(4): 322-329. [DOI:10.4103/1735-5362.212050]
18. Minaiyan M, Sadraei H, Yousefi I, Sajjadi S-E and Talebi A. Evaluation of the effect of hydroalcoholic and flavonoid-enriched extracts of Dracocephalum kotschyi on indomethacin-induced gastric ulcer in rats. Res. Pharm. Sci. 2021; 16(2): 141-152. [DOI:10.4103/1735-5362.310521]
19. Sadraei H, Ghanadian SM, Asghari GhR and Gavahian AR. Bronchodilator effect of apigenin and luteolin, two components of Dracocephalum kotschyi on isolated rabbit trachea. JHP. 2019; 8(4): 281-286. [DOI:10.15171/jhp.2019.41]
20. Sadraei H, Sajjadi SE and Tarafdar A. Antispasmodic effect of hydroalcoholic and flavonoids extracts of Dracocephalum kotschyi on rabbit bladder. JHP. 2020; 9(2): 145-152. [DOI:10.34172/jhp.2020.19]
21. Yu H, Chen Zh, Chen H and Wang Zh. Dracocephalum moldavica L. extract ameliorates intestinal inflammation by regulating gut microbiota and repairing the intestinal barrier in 2K1C rats. Arch. Biol. Sci. 2023; 75(2): 155-164. [DOI:10.2298/ABS230307013Y]
22. Zhang Q-W, Lin L-G and Ye W-C. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Med. 2018; 13(1): 20. doi: 10.1186/s13020-018-0177-x. [DOI:10.1186/s13020-018-0177-x]
23. Sadraei H, Arzi B and Yegdaneh A, The Effect of essential oil and Hydroalcoholic extract of Dracocephalum kotschyi on isolated heart and blood vessel contractions. JIMS. 2024; 41(744): 1030-1038.
24. Samuelsson G. Drugs of natural origin. Stockholm: Swedish Pharmaceutical Society. 6th ed. 2004, Swedish Pharmaceutical Press.
25. Sadraei H, Asghari G and Kasiri F. Comparison of antispasmodic effects of Dracocephalum kotschyi essential oil, limonene and α-terpineol. Res. Pharm. Sci. 2015; 10(2): 109-116.
26. National Research Council. Guide for the care and use of laboratory animals. 8th ed. National Academies Press (US). 2010.
27. Irwin MR, Curay CM, Choi Sh and Kiyatkin EA. Basic physiological effects of ketamine-xylazine mixture as a general anesthetic preparation for rodent surgeries. Brain Res. 2023; 1804: 148251. [DOI:10.1016/j.brainres.2023.148251]
28. Katori N, Yamakawa K, Kida K, Kimura Y, Fujioka Sh and Tsubokawa T. The incidence of hypotension during general anesthesia: A single-center study at a university hospital. 2023; 9(1): 23. [DOI:10.1186/s40981-023-00617-9]
29. Flahault A, Keck M, Girault-Sotias P-E, Esteoulle L, De Mota N, Bonnet D and Llorens-Cortes C. LIT01-196, a metabolically stable Apelin-17 analog, normalizes blood pressure in hypertensive DOCA-Salt rats via a NO Synthase-dependent mechanism. Front. Pharmacol. 2021; 12: 715095. [DOI:10.3389/fphar.2021.715095]
30. Sadaf F, Shamim S, Ishaq H, ur-Rehman A, Younus I and Siddiqui F. Evaluation of hypotensive and vasodilatory effects of Murraya koenigii leaves extract in L-NAME induced hypertensive rats in relation with Muscarinic receptors. 2024; 14(2): 359-364. [DOI:10.5530/ijpi.14.2.44]
31. Underwood W and Anthony R. AVMA guidelines for the euthanasia of animals: 2020ed. American Veterinary Medical Association. 2020.
32. Carey RM, Moran AE and Whelton PK. Treatment of hypertension: A review. JAMA. 2022; 328(18): 1849-1861. [DOI:10.1001/jama.2022.19590]
33. Wang J, Liu Y-M, Hu J and Chen C. Potential of natural products in combination with arsenic trioxide: Investigating cardioprotective effects and mechanisms. Biomed. Pharmaco. 2023; 162: 114464. [DOI:10.1016/j.biopha.2023.114464]
34. Feng Y, Zhou J, Zhong M, Ma D, Mao J, Liu F, Jiang Ch, Wu X and Jiang L. Plant-derived natural products ameliorating hypertension via signaling pathways: A review. Am. J. Chin. Med. 2025; 53(05): 1309-1353. [DOI:10.1142/S0192415X2550051X]
35. Jones KE, Hayden ShL, Meyer HR, Sandoz JL, Arata WH, Dufrene K, Ballaera C, Torres YL, Griffin P, Kaye AM, Shekoohi S and Kaye AD. The evolving role of Calcium channel blockers in hypertension management: pharmacological and clinical considerations. Curr. Issues Mol. Biol. 2024; 46(7): 6315-6327. [DOI:10.3390/cimb46070377]
36. Zohny YM, Awad SM, Alsaidan O and Rabie MA. Development of nifedipine isosteres: an integrated approach to the design, synthesis, and biological assessment of Calcium channel blockers. Front. Chem. 2025; 13: 1581037. [DOI:10.3389/fchem.2025.1581037]
37. Sadraei H, Asghari GhR and Alinejad M. Comparison of antispasmodic effect of hydroalcoholic extract of Dracocephalum kotschyi Boiss. in rat uterus and ileum. Res. Pharm. Sci. 2016; 11(4): 284-92. [DOI:10.4103/1735-5362.189295]
38. Gonçalves T, Almeida A, Pontes L, Oliveira J, Feitosa M, Júnior J, Veras R and Medeiros I. Monoterpenes in vascular function: A review of bioactivity and mechanisms of action. IJMS. 2025; 26(18). [DOI:10.3390/ijms26189243]
39. Alves-Silva, J.M., et al., The role of essential oils and their main compounds in the management of cardiovascular disease risk factors. Molecules. 2021; 26(12): 3506. [DOI:10.3390/molecules26123506]
40. Al-Harrasi A, Bhatia S, Behi T and Kaushik, Effects of essential oils on CNS, In role of essential oils in the management of COVID-19. CRC Press. 1st ed. 2022, 269-297. [DOI:10.1201/9781003175933-20]
41. Heghes SC, Vostinaru O, Rus LM, Mogosan C, Iuga CA and Filip L. Antispasmodic effect of essential oils and their constituents: A review. 2019; 24(9): 1675. [DOI:10.3390/molecules24091675]
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