year 23, Issue 92 (11-2024)
J. Med. Plants 2024, 23(92): 83-97 |
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Aghaei F, Shokrpour M, Nazeri V, Shaltouki-Rizi M. Interspecific hybridization in Thymus species: molecular and morphological characterization. J. Med. Plants 2024; 23 (92) :83-97
URL: http://jmp.ir/article-1-3692-en.html
URL: http://jmp.ir/article-1-3692-en.html
1- Department of Horticultural Science, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2- Department of Horticultural Science, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran ,shokrpour.majid@gmail.com
3- Department of Agronomy, College of Agriculture, Purdue University, West Lafayette, Indiana, USA
2- Department of Horticultural Science, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran ,
3- Department of Agronomy, College of Agriculture, Purdue University, West Lafayette, Indiana, USA
Abstract: (186 Views)
Background: Thyme is a famous medicinal plant from the Lamiaceae family, which is grown in the wild throughout Iran and has one of the world's top ten essential oils. Objective: In this study, four species of the Thymus genus including T. lancifolius, T. daenensis, T. vulgaris, T. kotschyanus were crossed. Out of about 2,000 crosses, 70 seeds were obtained. Molecular and morphological assessments were done on hybrid seeds. Parents and hybrids were compared in terms of some morphological traits. Methods: DNA from leaf samples of both parents and hybrids were extracted. SSR markers including 23 pairs of primers and ISSR markers with 20 primers were used for hybrid identification. Out of 23 primers used for SSR analysis, five primers yielded scorable amplified products for hybrids in their respective parental lines. Results: Stem length in hybrids increased, and internode length decreased. Primer LT12, with 66.66% identification, was introduced as the best primer for hybrid identification. Also, out of 19 ISSR primers, five primers confirmed hybrids and their parents. Primer ISCS43 with 100% ability to identify hybrids was introduced as the best primer. Conclusion: In general, all hybrids characterized by morphological characters were supported by the ISSR and SSR data. These results indicate that ISSR and SSR data will be valuable tools to verify hybrids.
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2024/06/30 | Accepted: 2025/03/4 | Published: 2024/11/30
Received: 2024/06/30 | Accepted: 2025/03/4 | Published: 2024/11/30
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