year 22, Issue 85 (3-2023)
J. Med. Plants 2023, 22(85): 113-127 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Lohani I, Sharma K. Quantitative analysis and biological activities of Bauhinia vahlii Wight & Arn. growing in Nepal. J. Med. Plants 2023; 22 (85) :113-127
URL: http://jmp.ir/article-1-3493-en.html
URL: http://jmp.ir/article-1-3493-en.html
1- Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal
2- Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal , khaga.sharma@cdc.tu.edu.np
2- Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal , khaga.sharma@cdc.tu.edu.np
Abstract: (1200 Views)
Background: The genus Bauhinia consists of about 300 species and is widely distributed in most tropical countries of the world. Objectives: The antioxidant, antibacterial, antidiabetic, and quantitative analysis of stem, leaf, and seed extracts of B. vahlii (the synonym name of Phanera vahlii) were investigated. Methods: The antioxidant activity of the crude extracts and fractions was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The antidiabetic activity was investigated by α-amylase inhibition assay. The toxicity of plant extracts was assessed by brine shrimp lethality assay (BSLA) using Artemia salina as a biological test organism. Results: The aqueous fraction of the leaf showed a high total phenolic content of 168.47 ± 1.94 mg GAE/g. The total flavonoid content was found maximum in an aqueous fraction of leaf with 158.51 ± 2.99 mg QE/g. The stem extract showed potent antioxidant activity with IC50 1.91 ± 0.33 µg/ml as compared to the standard quercetin IC50 3.46 ± 0.40 µg/ml. The DCM (Dichloromethane) fraction of the leaf exhibited noteworthy α-amylase inhibition properties with IC50 112.70 ± 2.0 µg/ml as compared to the standard acarbose of 9.34 ± 2.0 µg/ml. The antimicrobial results showed that the methanolic extract of stem and seed exhibited the zone of inhibition against Staphylococcus aureus, Bacillus subtilis, and Klebsiella pneumoniae but failed against E. coli. The methanol and aqueous extract of the leaf showed toxicity against brine shrimp nauplii with LC50 257.63 µg/ml and 100 µg/ml, respectively. Conclusion: This study showed Bauhinia vahlii is rich in flavonoid and phenolic content and the plant may be a rich source of natural antioxidants and antidiabetic agents that could be isolated as the drug candidate.
Keywords: Bauhinia vahlii, Phanera vahlii, Pharmacological activities, Antioxidant, α-Amylase, Antidiabetic
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2023/01/19 | Accepted: 2023/02/25 | Published: 2023/03/1
Received: 2023/01/19 | Accepted: 2023/02/25 | Published: 2023/03/1
References
1. Bodeker G and Graz B. 25-Traditional Medicine. Hunter's Traditional Medicine and Emerging Infectious Diseases, Tenth Edit. Elsevier Inc., 2020; 194-199. [DOI:10.1016/B978-0-323-55512-8.00025-9]
2. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, Gargiulo G, Testa G, Cacciatore F, Bonaduce D and Abete P. Oxidative stress, aging, and diseases. Clin. Interv. Aging. 2018; 13: 757-772. [DOI:10.2147/CIA.S158513]
3. Lobo V, Patil A, Phatak A and Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev. 2010; 4: 118-126. doi: 10.4103/0973-7847.70902. [DOI:10.4103/0973-7847.70902]
4. Mellitus DD. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014; 37, S81-S90. [DOI:10.2337/dc14-S081]
5. WHO, Global Report on Diabetes 2016; Isbn, vol. 978, 6-86. [Online]. Available: https://apps. who.int/ iris/handle/10665/204871.
6. Crawford K. Review of 2017 Diabetes Standards of Care. Nurs. Clin. North Am. 2017; 52(4): 621-663. [DOI:10.1016/j.cnur.2017.07.010]
7. Elosta A, Ghous T and Ahmed N. Natural products as Anti-glycation agents: possible therapeutic potential for diabetic complications. Curr. Diabetes Rev. 2012; 8(2): 92-108. [DOI:10.2174/157339912799424528]
8. Chaudhury A, Duvoor C, Reddy Dendi VS, Kraleti S, Chada A, Ravilla R, Marco A, Shekhawat NS, Montales MT, Kuriakose K, Sasapu A, Beebe A, Patil N, Musham ChK, Lohani GP and Mirza W. Clinical review of antidiabetic drugs implications for type 2 diabetes mellitus management. Front. Endocrinol. 2017; 8. [DOI:10.3389/fendo.2017.00006]
9. Agarwal P and Gupta R. Alpha-amylase inhibition can treat diabetes mellitus. RRJMHS 2016; 5(4): 1-8.
10. Mahmood N. A review of α-amylase inhibitors on weight loss and glycemic control in pathological state such as obesity and diabetes. Comp. Clin. Path. 2016; 25: 1253-1264. [DOI:10.1007/s00580-014-1967-x]
11. Tanika T, Vaishnavi, Ravinder K, Shruti S and Rakesh T. A review on: Bauhinia variegata and its phytoconstituents. Ayushdhara 2022; 9(3), 94-99. [DOI:10.47070/ayushdhara.v9i3.942]
12. Source SB, Conservatory PT and Garden B. Lectotypification of Bauhinia phoenicea Wight & Arn. (Leguminosae : Caesalpinioideae). Environm. Sci. 2012; 67: 41-43. [DOI:10.15553/c2012v671a5]
13. Mishra A, Sharma AK, Kumar S, Saxena AK and Pandey AK. Bauhinia variegata Leaf extracts exhibit considerable antibacterial, antioxidant, and anticancer activities. Biomed. Res. Int. 2013; 2013. 915436. [DOI:10.1155/2013/915436]
14. Uprety Y, Poudel RC, Shresth, KK, Rajbhandary S, Tiwari NN, Shrestha UB and Asselin H. Diversity of use and local knowledge of wild edible plant resources in Nepal. J. Ethnobiol. Ethnomed. 2012; 8(16): [DOI:10.1186/1746-4269-8-16]
15. Mohanty N, Das PK and Panda T. Use of plant diversity in household and rituals by tribal people of Dhenkanal district, Odisha, India. J. Appl. Pharm. Sci. 2011; 1(4): 79-82.
16. Mehta PS and Bhatt KC. Traditional soap and detergent yielding plants of Uttaranchal. Indian J. Tradit. Know. 2007; 6(2): 279-284.
17. Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI and BahorunT. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. JMRFMMM. 2005; 579(1-2): 200-213. [DOI:10.1016/j.mrfmmm.2005.03.023]
18. Shraim AM, Ahmed TA, Rahman MM and Hijji YM. Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT. 2021; 150: 111932. [DOI:10.1016/j.lwt.2021.111932]
19. Jamuna S, Paulsamy S and Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). JAPS. 2012; 2(7): 149-154, 2012. [DOI:10.7324/JAPS.2012.2722]
20. Wickramaratne MN, Punchihewa JC and Wickramaratne DBM. In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complem. Altern. M. 2016; 16(466): 1-5. [DOI:10.1186/s12906-016-1452-y]
21. Khanal LN, Sharma KR, Pokharel YR and Kalauni SK. Assessment of phytochemical, antioxidant and antimicrobial activities of some medicinal plants from Kaski district of Nepal. AJPS 2020; 11(9). [DOI:10.4236/ajps.2020.119099]
22. Neto MM, Neto MA, Filho RB, Lima MAS and Silveira ER. Flavonoids and alkaloids from leaves of Bauhinia ungulata L. Biochem. Syst. Ecol. 2008; 36(3): 227-229. [DOI:10.1016/j.bse.2007.08.006]
23. Negi A, Sharma N, Pant R and Singh MF. Determination of total phenolic content of the stem bark of Bauhinia variegata Linn.; an approach to standardization. J. Pharm. Res. 2012; 7(2): 16-22.
24. Fresco P, Borges F, Diniz C and Marques MPM. New Insights on the anticancer properties of dietary polyphenols. Med. Res. Rev. 2006; 6: 747-766. [DOI:10.1002/med.20060]
25. Farag MA, Sakna ST, El-fiky NM, Shabana MM and Wessjohann LA. Phytochemical, antioxidant and antidiabetic evaluation of eight Bauhinia L. species from Egypt using UHPLC-PDA-qTOF-MS and chemometrics. Phytochemistry 2015; 119: 41-50. [DOI:10.1016/j.phytochem.2015.09.004]
26. Kumar V and Roy BK. Population authentication of the traditional medicinal plant Cassia tora L. based on ISSR markers and FTIR analysis. Sci. Rep. 2018; 8(10714): 1-11. [DOI:10.1038/s41598-018-29114-1]
27. Lianah L, Nurkhasanah RA, Sari FR, Windayani KN and Masri M. Antioxidant activity of ethanolic extract of Bauhinia scandens. EEC 2022; 28: 595-601. [DOI:10.53550/EEC.2022.v28i02.002]
28. Anaya-Esparza LM, González-Silva N, Yahia EM, González-Vargas OA, Montalvo-González E and Pérez-Larios A. Effect of TiO2-ZnO-MgO mixed oxide on microbial growth and toxicity against Artemia salina. Nanomaterials. 2019; 9(7): [DOI:10.3390/nano9070992]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
