year 22, Issue 85 (3-2023)                   J. Med. Plants 2023, 22(85): 98-112 | Back to browse issues page

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Sabzi-Nojadeh M, Aharizad S, Mohammadi S A, Amani M. Screening of several important compounds production in fennel (Foeniculum vulgare Mill.) populations. J. Med. Plants 2023; 22 (85) :98-112
1- Department of Horticultural Science and Engineering, Ahar Faculty of Agriculture and Natural Resources, University of Tabriz, Tabriz, Iran ,
2- Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3- Department of Horticultural Science and Engineering, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Abstract:   (561 Views)
Background: The perennial fennel plant is one of the most important and widely used medicinal plants of the Apiaceae family, which is mainly cultivated for the purpose of using the essential oil obtained from it in various pharmaceutical, food, cosmetic and health industries. Objective: To investigate callogenesis and secondary metabolites production from calli, 13 populations of Iranian fennel as well as two populations from Germany and two populations from Turkey were evaluated by GC-MS. Methods: Five types of explants (leaf, hypocotyl, epicotyl, cotyledon, and root segments) were cultured in MS medium supplemented with combination of 2,4-D + Kinetin (1:1), and also NAA + BA (0.5:1). Results: Among five explants used in this study, only hypocotyl explant had appropriate response to the callogenesis. According to the GC-MS analysis, the highest trans-anethole content (67.23 %) was produced in the callus of Turkish population (TUR1) under NAA + BA treatment (1:1). Callus extracts of other Turkish population (TUR2) contained considerable amounts of limonene (67.70 %) under 2,4-D + Kinetin treatment (0.5:1). Conclusion: Callus induction with different plant regulators can have a significant contribution to the production of secondary metabolites, so callus that produce more secondary metabolites can be cultured in suspension or cell culture bioreactor systems. Based on the results of this research, Turkish fennel populations had the capacity to produce significant amounts of main secondary metabolites.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2023/01/9 | Accepted: 2023/02/28 | Published: 2023/03/1

1. Salami M, Rahimmalek M and Ehtemam MH. Comprehensive research on essential oil and phenolic variation in different Foeniculum vulgare populations during transition from vegetative to reproductive stage. Chemistry & Biodiversity 2016; 14(2): e1600246. [DOI:10.1002/cbdv.201600246]
2. Khan RU, Fatima A, Naz S, Ragni M, Tarricone S and Tufarelli V. Perspective, opportunities and challenges in using fennel (Foeniculum vulgare Mill.) in poultry health and production as an eco-friendly alternative to antibiotics: a review. Antibiotics 2022; 11(2): 278. [DOI:10.3390/antibiotics11020278]
3. Ahmed AF, Shi M, Liu C and Kang W. Comparative analysis of antioxidant activities of essential oils and extracts of fennel (Foeniculum vulgare Mill.) seeds from Egypt and China. Food Science and Human Wellness 2019; 8(1): 67-72. [DOI:10.1016/j.fshw.2019.03.004]
4. Jan R, Asaf S, Numan M and Kim KM. Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy 2021; 11(5): 968. [DOI:10.3390/agronomy11050968]
5. Liu Y, Li Y, Luo W, Liu S, Chen W, Chen C, Jiao S and Wei G. Soil potassium is correlated with root secondary metabolites and root-associated core bacteria in licorice of different ages. Plant and Soil 2020; 456: 61-79. [DOI:10.1007/s11104-020-04692-0]
6. Cardoso JC, Oliveira MEBS de and Cardoso FdC. Advances and challenges on the in vitro production of secondary metabolites from medicinal plants. Hortic. Bras. 2019; 37(2): 124-132. [DOI:10.1590/s0102-053620190201]
7. Chandana BC, Nagaveni HC, Lakshmana D, Kolakar SS and Heena MS. Role of plant tissue culture in micropropagation, secondary metabolites production and conservation of some endangered medicinal crops. JPP. 2018; 35(7): 246-251.
8. Hashim M, Ahmad B, Drouet S, Hano C, Abbasi BH and Anjum S. Comparative effects of different light sources on the production of key secondary metabolites in plants in vitro cultures. Plants 2021; 10(8): 1521. [DOI:10.3390/plants10081521]
9. Murashige T and Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 1962; 15(3): 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
10. Espinosa-Leal CA, Puente-Garza CA and García-Lara S. In vitro plant tissue culture: means for production of biological active compounds. Planta 2018; 248: 1-18. [DOI:10.1007/s00425-018-2910-1]
11. Babich O, Sukhikh S, Pungin A, Ivanova S, Asyakina L and Prosekov A. Modern trends in the in vitro production and use of callus, suspension cells and root cultures of medicinal plants. Molecules 2020; 25(24): 5805. [DOI:10.3390/molecules25245805]
12. Le V, Sukhikh A, Larichev T, Ivanova S, Prosekov A and Dmitrieva A. Isolation of the main biologically active substances and phytochemical analysis of Ginkgo biloba callus culture extracts. Molecules 2023; 28(4): 1560. [DOI:10.3390/molecules28041560]
13. Chang HC, Xie HM, Lee MR, Lin CY, Yip MK, Agrawal DC and Tsay HS. In vitro propagation of bulblets and LC-MS/MS analysis of isosteroidal alkaloids in tissue culture derived materials of Chinese medicinal herb Fritillaria cirrhosa D. Don. Botanical Studies 2020; 61: 1-9. [DOI:10.1186/s40529-020-00286-2]
14. Tabibazar S, Aharizad S, Uliaie ED, Sabzi Nojadeh M and Kosari-Nasab M. Effect of plant regulations on Callus essential oil content of Fennel (Foeniculum Vulgare Mill.) populations. J. Biochem. Tech. Special. 2020; 11(1): 141-145.
15. Scariolo F, Palumbo F and Barcaccia G. Molecular characterization and genetic structure evaluation of breeding populations of Fennel (Foeniculum vulgare Mill.). Agro. 2022; 12(3): 542. [DOI:10.3390/agronomy12030542]
16. Anzidei M, Bennici A, Schiff S, Tani C and Mori B. Organogenesis and somatic embryogenesis in Foeniculum vulgare: histological observations of developing embryogenic Callus. Plant Cell, Tissue and Organ. Culture 2000; 61(1): 69-79. [DOI:10.1023/A:1006454702620]
17. Afify AMR, El-Beltagi HS, Hammama AAE, Sidky MM and Mostafa OFA. Distribution of trans-anethole and estragole in fennel (Foeniculum vulgare Mill) of callus induced from different seedling parts and fruits. Notulae Scientia Biologicae 2011; 3(1): 79-86. [DOI:10.15835/nsb315422]
18. Paupardin C, Garcia-Rodriguez M. J, Bricout J. Application of in vitro culture to improve vegetables. Tissue culture of aromatic plants: essential oil production, vegetative propagation. Colloq. Eucarpia, Versailles, C.N.R.A. ed., 1980; 201-210.
19. Wesołowska A, Jadczak P, Kulpa D and Przewodowski W. Gas chromatography-mass spectrometry (GC-MS) analysis of essential oils from AgNPs and AuNPs elicited Lavandula angustifolia in vitro cultures. Molecules 2019; 24(3): 606. [DOI:10.3390/molecules24030606]
20. Ma C, Goddard A, Peremyslova E, Duan C, Jiang Y, Nagle M and Strauss SH. Factors affecting in vitro regeneration in the model tree Populus trichocarpa I. Medium, environment, and hormone controls on organogenesis. In vitro Cellular & Developmental Biology-Plant 2022; 58: 837-852. [DOI:10.1007/s11627-022-10301-9]
21. Shahi S, Izadi-Darbandi A, Ramshini H and Younessi-hamzekhanlu M. Rapid and high throughput regeneration in fennel (Foeniculum vulgare Mill.) from embryo explants. J. Plant Mol. Breed. 2017; 5(2): 11-19.
22. Abdi N, Uliaie E. D, Bandehagh A and Aharizad S. Effect of Agrobacterium rhizogenesis on hairy roots induction in Fennel (Foeniculum vulgare Miller). JEBAS. 2017; 5(3): 384-391. [DOI:10.18006/2017.5(3).384.391]
23. Bahmankar M, Mortazavian SMM, Tohidfar M, Sadat Noori SA, Izadi Darbandi A, Corrado G and Rao R. Chemical compositions, somatic embryogenesis, and somaclonal variation in cumin. Chemical compositions, somatic embryogenesis, and somaclonal variation in cumin. BioMed Res. International 2017; 1-15. [DOI:10.1155/2017/7283806]

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