year 21, Issue 83 (9-2022)                   J. Med. Plants 2022, 21(83): 60-71 | Back to browse issues page

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Kazemi D, Nejad Ebrahimi S, Mohsenian Kouchaksaraee R. Fabrication and optimization of physicochemical properties of nano-phytosome from Punica granatum L. peel enriched polyphenol extract. J. Med. Plants 2022; 21 (83) :60-71
1- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
2- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran ,
Abstract:   (294 Views)
Background: Punica granatum L. peel is a rich source of phenolic compounds such as punicalagin and ellagic acid that represent a major part of the fruit and many fruit by-products in Asian countries, mainly Iran. Objective: The purpose of this study is to design a value-added pomegranate peel product. Methods: For this purpose, a safe and environmentally friendly method of resin column chromatography was used to enrich the hydroalcoholic extract of pomegranate peel with punicalagin. The enrichment conditions were optimized in terms of column condition and the amount of extract used. The results were monitored and confirmed by HPLC-PDA. In order to improve the physicochemical properties of the enriched extract, the obtained extract was loaded onto nano-phytosome by thin-film hydration method. FT-IR confirmed the nano-phytosome structure. Results: The resin column was optimized at a 3:1 resin-to-extract ratio in an optimum contact time duration of 30 minutes (80.50 %) and a 2.63-fold increase in the phenolic compounds compared to the crude extract. The results indicated that encapsulation efficiency was equal to 58 %, the particle size was equal to 154.0 to 216.5 nm, and one-year stability was achieved with an optimal polydispersity index of 0.215-0.287 in terms of particle size. Conclusion: The enrichment of the crude extract before formulation and fabrication polyphenol enriched extract is a promising strategy to transform of pomegranate peel into a valuable product.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2022/07/10 | Accepted: 2022/08/29 | Published: 2022/10/3

1. Munin A and Edwards-Lévy F. Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics 2011; 3(4): 793-829. [DOI:10.3390/pharmaceutics3040793]
2. Basli A, Belkacem N and Amrani I. Health benefits of phenolic compounds against cancers. Phenolic compounds-biological activity. London: InTechOpen, 2017; 8: 193-210. [DOI:10.5772/67232]
3. Zhai XC, Zhu C.P, Zhang Y, Sun JR, Alim A and Yang X B. Chemical characteristics, antioxidant capacities and hepatoprotection of polysaccharides from pomegranate peel. Carbohydr. Polym. 2018; 202: 461-469. [DOI:10.1016/j.carbpol.2018.09.013]
4. Akhtar S, Ismail T, Fraternale D and Sestili P. Pomegranate peel and peel extracts: chemistry and food features. Food Chem. 2015; 174: 417-425. [DOI:10.1016/j.foodchem.2014.11.035]
5. Khan JA and Hanee S. Antibacterial properties of Punica granatum peels. Int. J. Appl. Biol. Pharm. 2011; 2(3): 23-27.
6. Chen J, Liao C L, Ouyang XL, Kahramanoglu I, Gan Y D and Li MX. Antimicrobial activity of pomegranate peel and its applications on food preservation. J. Food Qual. 2020; 2020: 8850339. [DOI:10.1155/2020/8850339]
7. Fathi F, Ebrahimi S.N, Valadao A I G, Andrade N, Costa A S G, Silva C, Fathi A, Salehi P, Martel F, Alves R C and Oliveira M B P P. Exploring Gunnera tinctoria: from nutritional and anti-tumoral properties to phytosome development following structural arrangement based on molecular docking. Molecules 2021; 26(19): 5935, 1-27. [DOI:10.3390/molecules26195935]
8. Fathi F and Ebrahimi S N. Investigation of physiochemical properties nanophytosome obtained from of polyphenolic enrich fraction of Satureja khuzistanica by freeze-drying. Nashrieh Shimi va Mohandesi Shimi Iran 2021.
9. Karakaya S. Bioavailability of phenolic compounds. Crit. Rev. Food Sci. Nutr. 2004; 1; 44(6): 453-64. [DOI:10.1080/10408690490886683]
10. Gaikwad A R, Ahire K D, Gosavi A A, Salunkhe K S and Khalkar A. Phytosome as a Novel Drug Delivery System for Bioavailability Enhancement of Phytoconstituents and its Applications: A Review. J. Drug Del. Therapy. 2021; 11(3): 138-152. [DOI:10.22270/jddt.v11i3.4847]
11. Fathi F, Ebrahimi S N, Pereira D M, Estevinho B N and Rocha F. Preliminary studies of microencapsulation and anticancer activity of polyphenols extract from Punica granatum peels. Can. J. Chem. Eng. 1.
12. Fathi F, Ebrahimi S N, Pereira D M, Estevinho B N and Rocha F. Microencapsulation of enriched extracts of two Satureja species by spray drying, evaluation of the controlled release mechanism and cytotoxicity. Pharm. Sci. 2021; 28(1): 145-155. [DOI:10.34172/PS.2021.54]
13. Fathi F, Ebrahimi S N, Prior J A V, Machado S M L, Kouchaksaraee R M, Oliveira M B P P and Alves R C. Formulation of nano/micro-carriers loaded with an enriched extract of coffee silverskin: physicochemical properties, in vitro release mechanism and in silico molecular modeling. Pharmaceutics 2022; 14(1): 112. [DOI:10.3390/pharmaceutics14010112]
14. Costa P, Manuel J and Lobo S. Modeling and comparison of dissolution profiles. Eur. J. Phar. Sci. 2001; 13(2): 123-133. [DOI:10.1016/S0928-0987(01)00095-1]
15. Permanadewi I, Kumoro A C, Wardhani D H and Aryanti N. Modelling of controlled drug release in gastrointestinal tract simulation. J. Phys. Conf. Ser. 2019; 1295(2019): 012063. [DOI:10.1088/1742-6596/1295/1/012063]
16. Ruan J H.; Li J Adili G, Sun GY, Abuduaini M, Abdulla R, Maiwulanjiang M and Aisa HA. Phenolic compounds and bioactivities from pomegranate (Punica granatum L.) peels. J. Agri. Food Chem. 2022; 70(12): 3678-3686. [DOI:10.1021/acs.jafc.1c08341]
17. Danaei M, Dehghankhold M, Ataei S, Davarani F H, Javanmard R, Dokhani A, Khorasani S and Mozafari M R. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics 2018; 10(2): 57. [DOI:10.3390/pharmaceutics10020057]
18. Campos J C, Ferreira D C, Lima S, Reis S and Costa P J. Swellable polymeric particles for the local delivery of budesonide in oral mucositis. Int. J. Pharm. 2019; 566: 126-140. [DOI:10.1016/j.ijpharm.2019.05.057]
19. Susilawati Y, Chaerunisa AY and Purwaningsih H. Phytosome drug delivery system for natural cosmeceutical compounds: Whitening agent and skin antioxidant agent. J. Adv. Pharm. Technol. Res 2021; 12(4): 327-324. [DOI:10.4103/japtr.JAPTR_100_20]
20. Zhang JF, Tang Q, Xu XY and Li, N. Development and evaluation of a novel phytosome-loaded chitosan microsphere system for curcumin delivery. Int. J. Pharm. 2013; 448(1): 168-174. [DOI:10.1016/j.ijpharm.2013.03.021]

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