year 21, Issue 82 (5-2022)
J. Med. Plants 2022, 21(82): 111-118 |
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Latifi R, Azadmehr A, Mosalla S, Saleki K, Hajiaghaee R. Scolicidal effects of the Nicotiana tabacum L. extract at various concentrations and exposure times. J. Med. Plants 2022; 21 (82) :111-118
URL: http://jmp.ir/article-1-3309-en.html
URL: http://jmp.ir/article-1-3309-en.html
1- Parasitology Department, Qazvin University of Medical Sciences, Qazvin, Iran
2- Immunology Department, Babol University of Medical Sciences, Babol, Iran & Immunology Department, Qazvin University of Medical Sciences, Qazvin, Iran ,a.azadmehr@mubabol.ac.ir
3- Student Research Committee, Babol University of Medical Sciences, Babol, Iran & USERN Office, Babol University of Medical Sciences, Babol, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Immunology Department, Babol University of Medical Sciences, Babol, Iran & Immunology Department, Qazvin University of Medical Sciences, Qazvin, Iran ,
3- Student Research Committee, Babol University of Medical Sciences, Babol, Iran & USERN Office, Babol University of Medical Sciences, Babol, Iran
4- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
Abstract: (2155 Views)
Background: Tobacco is a plant that has anti-nematode and anti-inflammatory properties, due to that it contains compounds such as nicotine sulfate. It is utilized as a medicinal plant in the therapy of infectious diseases; also, it has been used to kill insects’ larvae and plant pests. Objective: The goal of this work was the evaluation of tobacco extract effects on the scolices of hydatid cysts. Methods: Hydatid cysts of the infected sheep livers were gathered from the Qazvin abattoir and their protoscolices were isolated under sterile conditions. Fertility and viability of protoscolices were confirmed before the experiments. The protoscolices were exposed to the ethanolic extract of Nicotiana tabacum, at different concentrations (3, 5, 10, 25, 50, and 100 mg/ml) and various exposure times (10, 20, 30, 40, 50 and 60 min). Sodium chloride 0.9 % was selected as negative control. The viability of protoscolices was evaluated by staining via 0.1 % eosin. Results: At 100 mg/ml, the tobacco extract caused the death of 91.19 % of protoscolices after 10 minutes of contact, and other exposure times (20 to 60 minutes) resulted in the death of 100 % of protoscolices. Conclusion: This medicinal plant showed a good scolicidal effect and it may be used for improving the procedure of hydatid cyst surgery. But, further studies are required.
Type of Study: Research |
Subject:
Medicinal Plants
Received: 2022/04/4 | Accepted: 2022/05/24 | Published: 2022/05/31
Received: 2022/04/4 | Accepted: 2022/05/24 | Published: 2022/05/31
References
1. Adas G, Arikan S, Kemik O, Oner A, Sahip N and Karatepe O. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World Journal of Gastroenterology: WJG. 2009; 15(1): 112. [DOI:10.3748/wjg.15.112]
2. Eckert J and Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin. Microbiol Rev. 2004; 17(1): 107-135. [DOI:10.1128/CMR.17.1.107-135.2004]
3. McManus DP, Zhang W, Li J and Bartley PB. Echinococcosis. The lancet. 2003; 362(9392): 1295-1304. [DOI:10.1016/S0140-6736(03)14573-4]
4. Prousalidis J, Kosmidis C, Anthimidis G, Kapoutzis K, Karamanlis E and Fachantidis E. Postoperative recurrence of cystic hydatidosis. Can. J. Surg. 2012; 55(1): 15-20. [DOI:10.1503/cjs.013010]
5. Rajabi MA. Fatal reactions and methaemoglobinaemia after silver nitrate irrigation of hydatid cyst. Surg. Pract. Practice. 2009; 13(1): 2-7. [DOI:10.1111/j.1744-1633.2008.00427.x]
6. Moazeni M, Saharkhiz MJ and Hosseini AA. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Vet. Parasitol. 2012; 187(1-2): 203-208. [DOI:10.1016/j.vetpar.2011.12.025]
7. Harris JC, Plummer S, Turner MP and Lloyd D. The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology. 2000; 146(12): 3119-3127. [DOI:10.1099/00221287-146-12-3119]
8. Moazeni M and Nazer A. In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World J. Surg. 2010; 34(11): 2677-2681. doi: 10.1007/s00268-010-0718-7. [DOI:10.1007/s00268-010-0718-7]
9. Zibaei M, Sarlak A, Delfan B, Ezatpour B and Azargoon A. Scolicidal effects of Olea europaea and Satureja khuzestanica extracts on protoscolices of hydatid cysts. Korean. J. Parasitol. 2012; 50(1): 53. [DOI:10.3347/kjp.2012.50.1.53]
10. Shahnazi M, Azadmehr A, Latifi R, Hajiaghaee R, Saraei M and Alipour M. In vitro protoscolicidal effects of various concentrations of Ziziphora tenuior L. extract at different exposure times. Avicenna J. Phytomed. 2016; 6(4): 376.
11. Moazeni M and Roozitalab A. High scolicidal effect of Zataria multiflora on protoccoleces of hydatid cyst: an in vitro study. Comparative Clinical Pathology. 2012; 21(1): 99-104. [DOI:10.1007/s00580-010-1069-3]
12. Maggiore MA, Albanese AA, Gende LB, Eguaras MJ, Denegri GM and Elissondo MC. Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitol. Res. 2012; 110(3): 1103-1112. [DOI:10.1007/s00436-011-2595-x]
13. Dawwas A. Investigation of biochemical effect of phenols extract isolated from Coriandrum sativum seeds against Echinococcus granulosus parasite in vitro. Univ. Thi-Qar J. Sci. 2008; 1(1): 2-9. [DOI:10.32792/thi/sc/vol.1.is.1]
14. Saleki K, Banazadeh M, Saghazadeh A and Rezaei N. The involvement of the central nervous system in patients with COVID-19. Rev. Neurosci. 2020; 31(4): 453-456. [DOI:10.1515/revneuro-2020-0026]
15. Saleki K, Banazadeh M, Miri NS and Azadmehr A. Triangle of cytokine storm, central nervous system involvement, and viral infection in COVID-19: the role of sFasL and neuropilin-1. Rev. Neurosci. 2022; 33(2): 147-160. [DOI:10.1515/revneuro-2021-0047]
16. Amirghofran Z, Bahmani M, Azadmehr A and Javidnia K. Anticancer effects of various Iranian native medicinal plants on human tumor cell lines. Neoplasma. 2006; 53(5): 428-433.
17. Amirghofran Z, Azadmehr A and Javidnia K. Haussknechtia Elymatica: A Plant with Immunomodulatory Effects. Iran. J. Immunol. 2007; 4(1): 26-31.
18. Saleki K, Yaribash S, Banazadeh M, Hajihosseinlou E, Gouravani M, Saghazadeh A and Rezaei N. Interferon therapy in patients with SARS, MERS, and COVID-19: A systematic review and meta-analysis of clinical studies. Eur. J. Pharmacol. 2021; 906: 174248. [DOI:10.1016/j.ejphar.2021.174248]
19. Díaz Á. Immunology of cystic echinococcosis (hydatid disease). Br Med Bull. 2017: 1-13. [DOI:10.1093/bmb/ldx033]
20. Zaman MA, Iqbal Z, Abbas RZ, Khan MN, Muhammad G, Younus M and Ahmed S. In vitro and in vivo acaricidal activity of a herbal extract. Vet. Parasitol. 2012; 186(3-4): 431-436. [DOI:10.1016/j.vetpar.2011.11.018]
21. Shaddel TA, Maheri SN, Agha JGA, Asaadi DA and Ahmadzadeh A. The study on the effect of tobacco, harmel and thymus plants on varroa, a honey bee parasite. Agroecol. J. 2008; 10(4) 67-72.
22. Mehta R and Anita A. A review on anthelmintic plants. Natural Product. Radiance. 2008; 7(5): 466-475.
23. Iqbal Z, Lateef M, Jabbar A, Ghayur MN and Gilani AH. In vitro and in vivo anthelmintic activity of Nicotiana tabacum L. leaves against gastrointestinal nematodes of sheep. Phytother Res. 2006; 20(1): 46-48. [DOI:10.1002/ptr.1800]
24. Bahmani M, Hosseini S, Avijgan M and Qorbani M. Evaluating the anti-leech effects of tobacco methanolic extract compared with succinyle choline and some other anti-parasite drugs. Journal of Shahrekord University of Medical Sciences. 2010; 12(3): 53-59.
25. Amirghofran Z, Bahmani M, Azadmehr A, Ashouri E and Javidnia K. Antitumor Activity and Apoptosis Induction in Human Cancer Cell Lines by Dionysia termeana. Cancer Invest. 2007; 25(7): 550-554. [DOI:10.1080/07357900701518487]
26. Tariku Y, Hymete A, Hailu A and Rohloff J. In vitro evaluation of antileishmanial activity and toxicity of essential oils of Artemisia absinthium and Echinops kebericho. Chem. Biodivers. 2011; 8(4): 614-623. [DOI:10.1002/cbdv.201000331]
27. Sooudi S, Hashemi S, Zavaran HA, Ghaemi A and Asghari JM. Antileishmanial effect of Echinacea purpurea root extract cultivated in Iran. IJPR 2007; 6(2): 147-149.
28. Smyth J and Barrett N. Procedures for testing the viability of human hydatid cysts following surgical removal, especially after chemotherapy. Trans. R. Soc. Trop. Med. Hyg. 1980; 74(5): 649-652. [DOI:10.1016/0035-9203(80)90157-1]
29. Dalimi A, Motamedi G, Hosseini M, Mohammadian B, Malaki H, Ghamari Z and Far FG. Echinococcosis/hydatidosis in western Iran. Vet. Parasitol. 2002; 105(2): 161-171. [DOI:10.1016/S0304-4017(02)00005-5]
30. Puryan K, Karadayi K, Topcu O, Canbay E, Sumer Z, Turan M, Karayalcin K and Sen M. Chlorhexidine gluconate: an ideal scolicidal agent in the treatment of intraperitoneal hydatidosis? World J. Surg. 2005; 29(2): 227-230. [DOI:10.1007/s00268-004-7587-x]
31. Kayaalp C, Balkan M, Aydin C, Ozgurtas T, Tanyuksel M, Kirimlioglu V, Akoglu M, Oner K and Pekcan M. Hypertonic Saline in Hydatid Disease. World J. Surg. 2001; 25(8): 975-979. [DOI:10.1007/s00268-001-0065-9]
32. Erzurumlu K, Hokelek M, Baris S, Sahin M, Birinci A, Amanvermez R and Tac K. Effect of Albendazole sulfoxide solution on the scolices and the hepatobiliary system. Eur. Surg. Res. 1998; 30(6): 433-438. [DOI:10.1159/000008610]
33. Caglar R, Yuzbasioglu MF, Bulbuloglu E, Gul M, Ezberci F and Kale IT. In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J. Invest. Surg. 2008; 21(2): 71-75. [DOI:10.1080/08941930701883640]
34. Besim H, Karayalçin K, Hamamci O, Güngör C and Korkmaz A. Scolicidal agents in hydatid cyst surgery. HPB Surg. 1998; 10(6): 347-351. [DOI:10.1155/1998/78170]
35. Rouhani S, Salehi N, Kamalinejad M and Zayeri F. Efficacy of Berberis vulgaris aqueous extract on viability of Echinococcus Granulosus protoscolices. J. Invest. Surg. 2013; 26(6): 347-351. [DOI:10.3109/08941939.2013.818746]
36. Adas G, Arikan S, Kemik O, Oner A, Sahip N and Karatepe O. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J. Gastroenterol. 2009; 15(1): 112-116. [DOI:10.3748/wjg.15.112]
37. Srivastava P, Singh M, Devi G and Chaturvedi R. Herbal Medicine and Biotechnology for the Benefit of Human Health. Animal Biotechnology Seventh Ed., Academic Press, Elsevier 2020; pp: 613-629. [DOI:10.1016/B978-0-12-811710-1.00028-8]
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