year 21, Issue 81 (3-2022)
J. Med. Plants 2022, 21(81): 1-11 |
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Tavakoli S, Khalighi-Sigaroodi F, Hagiaghaee R, Yaghoobi M, Ghafarzadegan R. Purification, identification, and standardization of silybin A & B composition from Silybum marianum (L.) Gaertn.. J. Med. Plants 2022; 21 (81) :1-11
URL: http://jmp.ir/article-1-3113-en.html
URL: http://jmp.ir/article-1-3113-en.html
Saeed Tavakoli1 
, Farahnaz Khalighi-Sigaroodi1 , Reza Hagiaghaee1 , Mahdi Yaghoobi2 , Reza Ghafarzadegan * 3
, Farahnaz Khalighi-Sigaroodi1 , Reza Hagiaghaee1 , Mahdi Yaghoobi2 , Reza Ghafarzadegan * 3
1- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran ,Ghafarzadegan@imp.ac.ir
2- Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
3- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran ,
Abstract: (2941 Views)
Background: Silybum marianum (L.) Gaertn. (Milk thistle) is a perennial herb with medicinal properties. The seeds of these plants contain silymarin compounds with flavonolignan structure and antioxidant, anti-inflammatory and hepatoprotective effects. The major bioactive constituent of S. marianum is silybin A and B. It is used in the treatment of various liver conditions and exhibits high anti-tumor promoting activity. Objective: The purpose of this study was to purify, identify, and standardize of silybin A and B from the seeds extract of Silybum marianum. Methods: At first, the milk thistle seeds were defatted with hexane and then extracted with methanol as solvent. Isolation and further purification of silybin A and B was carried out by column chromatography using Diaion HP-20 resin, silica gel and Sephadex LH-20 as stationary phase, respectively. 1H-NMR and 13C-NMR techniques were used to identify these compounds. Finally, the HPLC method has been used to standardize. Results: 1H-NMR and 13C-NMR techniques characterized the structure of silybin A and B extracted from Silybum marianum L. and standardization and determination of their purity was performed using HPLC. Conclusion: Our proposed system presented significant advantages in increasing efficiency and reducing cost, and the diastereoisomers of silybin A and silybin B in silymarin were successfully isolated with high purities.
Keywords: Silybum marianum, Silybin A, B, Column chromatography, Diaion HP-20, Sephadex LH-20, HPLC, NMR
Type of Study: Research |
Subject:
Pharmacognosy & Pharmaceutics
Received: 2021/05/21 | Accepted: 2021/11/20 | Published: 2022/03/1
Received: 2021/05/21 | Accepted: 2021/11/20 | Published: 2022/03/1
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