year 20, Issue 77 (1-2021)                   J. Med. Plants 2021, 20(77): 50-59 | Back to browse issues page

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Eradati N, Tajabadi F, Ahmadi-Ashtiani H R, Rezazadeh S, Taherian M, Rastegar H. Optimization of cleaning and analytical method for determination of arbutin, hydroquinone and kojic acid in cosmetic products. J. Med. Plants. 2021; 20 (77) :50-59
1- Islamic Azad University, Medical Sciences Branch, Faculty of pharmacy, Tehran, Iran
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- Cosmetic, Hygienic and Detergent Sciences and Technology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Food and Drug Laboratory Research Center, Iran Food and Drug Administration (FDA), Ministry of Health and Medical Education (MOH), Tehran, Iran
5- Cosmetic Products Research Center, Iran Food and Drug Administration (FDA), Ministry of Health and Medical Education (MOH), Tehran, Iran ,
Abstract:   (1065 Views)
Background: Hydroquinone, arbutin and kojic acid are herbal-based skin whitening compounds that are used in many cosmetics. Today, the lack of side effects of arbutin and kojic acid has made them a valuable alternative to hydroquinone. Due to the toxic effects of whitening compounds, it is necessary to control the amount of use of these in the whitening products so that their concentration is within the permissible range. Objective: Therefore, the purpose of this study was to identify and quantify these three compounds in anti-lacquer products. In this research, the best separation of analytes has been tried by changing the HPLC mobile phase. Methods: Different samples were taken from the supply level. After extraction and preparation of samples, the solution was injected into HPLC-UV. In order to optimize the method, three different mobile phases were used to identify the compounds. By comparing the obtained peaks with the standard peaks, the Whitening compounds were identified and quantified. Results: The results showed that hydroquinone and kojic acid were applied in three products and arbutin in one product and the concentration of all the compounds used were within the standard range. Four out of the eight companies surveyed did not correctly identify the compounds used in their products. Conclusion: Reverse Phase HPLC-UV is also one of the best methods for determination and quantification the whitening compounds and water composition: acetonitrile with a ratio of 80:20 v/v is the most appropriate mobile phase in this method.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2019/10/3 | Accepted: 2021/01/26 | Published: 2021/03/1

1. Thongchai W and Liawruangrath B. High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts. Cosmetic Sci. 2007; 58: 35-44. [DOI:10.1111/j.1468-2494.2007.00391_4.x]
2. Thogchai W and Liawruangrath B. Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic product. Cosmetic Sci. 2013; 35: 257-263. [DOI:10.1111/ics.12037]
3. Abere G. Assessment of levels of some active skin lightening compounds in selected facial creams and soaps in the Kenyan market. M. Sc. Thesis, University of Kenyatta, 2015.
4. Migas P and Baranowska M. The significance of arbutin and its derivatives in therapy and cosmetics. Phytochem. Lett. 2015, 13: 35-40. [DOI:10.1016/j.phytol.2015.05.015]
5. WHO, Exposure to mercury: A major public health concern. Geneva, World Health Organisation (Preventing disease through healthy environment), 2007.
6. FDA, Hydroquinone [CAS 123-31-9] Supporting Information for Toxicological Evaluation by the National Toxicology Program 21 May 2009.
7. Hong Wamg Y, Avonto C, Avula B and Wang M. Quantitative determination of α-arbutin, β-arbutin, kojic acid, nicotinamide, hydroquinone, resorcinol, 4-methoxyphenol, 4-ethoxyphenol, and ascorbic acid from skin whitening products by HPLC-UV. AOAC International. 2015; 98: 5-12. [DOI:10.5740/jaoacint.14-123]
8. Jeon JS, Lee MJ, Yoon MH, Park J-A, Yi H, Cho H-J and Shin H-C. Determination of arbutin, niacinamide, and adenosine in functional cosmetic products by high-performance liquid chromatography. Anal. Lett. 2014; 47: 1650-1660. [DOI:10.1080/00032719.2014.883517]
9. Jeon JS, Kim HT, Kim MG, Oh M-S, Hong S-R, Yoon M-H, Cho S-M, Shin H-C, Shim J-H, Ramadan A and Abd El-Aty A. M. Simultaneous determination of water-soluble whitening ingredients and adenosine in different cosmetic formulations by high-performance liquid chromatography coupled with photodiode array detection. Int. J. Cosmet. Sci. 2016; 38:286-93. [DOI:10.1111/ics.12292]
10. Kim T. J, Park Y. J, Park S. U, Ha S. H and Kim J. K. Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography-mass spectrometry. Applied Bio. Chem. 2018; 61: 523-530. [DOI:10.1007/s13765-018-0385-1]
11. Butwong N, Kunawong T and Luong JHT. Simultaneous analysis of hydroquinone, arbutin, and ascorbyl glucoside using a nanocomposite of Ag@AgCl nanoparticles, Ag2S nanoparticles, multiwall carbon nanotubes, and chitosan. Nanomaterials (Basel). 2020; 10: 1583-1596. [DOI:10.3390/nano10081583]
12. Zukepli NWM, Omar WSAW and Zakaria SR. Assessment on hydroquinone in selected cosmetic cream and toner via high performance liquid chromatography and ultra-violet visible detector spectrometry. Malaysian Journal of Analytical Sciences 2015, 19: 824-830.
13. Rychlinska I and Nowak S. Quantitative determination of arbutin and hydroquinone in different plant materials by HPLC. Not. Bot. Horti. Agrobo. 2012, 40: 109-113. [DOI:10.15835/nbha4027987]
14. Burnett CL and Bergfeld WF. Final report of the safety assessment of kojic acid as used in cosmetics. Toxicology 2010, 29: 244-273. [DOI:10.1177/1091581810385956]
15. Lopez Garcıa P, Rocha Miritello Santoro M.I., Kedor-Hackman E.R.M and Kumar Singh A. Development and validation of a HPLC and a UV derivative spectrophotometric methods for determination of hydroquinone in gel and cream preparations. J. Pharm. Biomed. Anal. 2005, 39: 764-768. [DOI:10.1016/j.jpba.2005.04.016]
16. Thongchai W, Liawruangrath B and Liawruangrath S. High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts. J. Cosmet. Sci. 2007, 58: 35-44. [DOI:10.1111/j.1468-2494.2007.00391_4.x]

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