year 19, Issue 73 (3-2020)                   J. Med. Plants 2020, 19(73): 109-118 | Back to browse issues page


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Momtaz S, Dibaj M, Abdollahi A, Amin G, Bahramsoltani R, Abdollahi M, et al . Wound healing activity of the flowers of Lilium candidum L. in burn wound model in rats. J. Med. Plants. 2020; 19 (73) :109-118
URL: http://jmp.ir/article-1-2631-en.html
1- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
2- Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3- Department of Pathology, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran
6- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
7- Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
8- Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , amirhosein172@hotmail.com
Abstract:   (934 Views)
Background: Ethnomedicinal plants are of interest for treatment of various wound types. Objective: This study aimed to evaluate burn wound healing potential of the hydro-alcoholic extract of the flowers of Lilium candidum L. in rats. Methods: DPPH free radical scavenging power, total flavonoids, and the phenolic content of the extract were evaluated. Following burn wound induction, 30 Wistar rats were divided into 5 groups; silver sulfadiazine cream 1% (reference standard), eucerin (control), and 5%, 10% and 20% ointments of L. candidum flowers extract (treatment groups). Wound contraction and the percentage of wound healing were measured every day until 14th day and the histological changes were evaluated. Results: DPPH assay of L. candidum extract indicated high antioxidant activity. Total phenolic content of L. candidum extract was equal to 157 mg gallic acid equivalent/g; while total flavonoid was measured as 32.4 mg quercetin equivalent/g dried extract. The mean of wound area was significantly different in three 5%, 10% and 20% extract-treated rats in comparison with the control. Although, 20% ointment of L. candidum flowers extract demonstrated the best wound healing improvement, even better than silver sulfadiazine. Histopathological analysis confirmed the wound healing activity of L. candidum and promoting tissue repair, especially for the ointment containing 20% extract. Conclusion: Regarding the high content of phenolic compounds and flavonoids, besides potent antioxidant properties of the flowers of L. candidum, the use of its ointments led to significant decrease of burn wound area, suggesting that these extract ointments can treat burn wounds and improve the healing processes.
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Type of Study: Research | Subject: Pharmacology & Toxicology
Received: 2019/08/20 | Accepted: 2020/01/7 | Published: 2020/06/6

References
1. Kahkeshani N, Farahanikia B, Mahdaviani P, Abdolghaffari A, Hassanzadeh G, Abdollahi M and Khanavi M. Antioxidant and burn healing potential of Galium odoratum extracts. Res. Pharm. Sci. 2013; 8 (3): 197.
2. Kwansang J, Itthipanichpong C and Limpanasithikul W. Evaluation of wound healing activity of Thunbergia laurifolia supercritical carbon dioxide extract in rats with second-degree burn wounds. J. Adv. Pharm. Technol. Res. 2015; 6 (3): 103. [DOI:10.4103/2231-4040.157984]
3. Hanson RR. Management of burn injuries in the horse. Veterinary Clinics of North America-Equine Practice. 2005; 21 (1): 105-24. [DOI:10.1016/j.cveq.2004.11.006]
4. Palmieri TL and Greenhalgh DG. Topical treatment of pediatric patients with burns. Am. J. Clin. Dermatol. 2002; 3 (8): 529-34. [DOI:10.2165/00128071-200203080-00003]
5. Fahimi S, Abdollahi M, Mortazavi SA, Hajimehdipoor H, Abdolghaffari AH and Rezvanfar MA. Wound healing activity of a traditionally used poly herbal product in a burn wound model in rats. Iran. Red Crescent. Med. J. 2015; 17 (9): e19960. [DOI:10.5812/ircmj.19960]
6. Tanideh N, Haddadi MH, Rokni-Hosseini MH, Hossienzadeh M, Mehrabani D, Sayehmiri K and et al. The healing effect of scrophularia striata on experimental burn wounds infected to pseudomonas aeruginosa in rat. WJPS. 2015; 4 (1): 16.
7. Esposito D, Munafo JP, Lucibello T, Baldeon M, Komarnytsky S and Gianfagna TJ. Steroidal glycosides from the bulbs of Easter lily (Lilium longiflorum Thunb.) promote dermal fibroblast migration in vitro. J. Ethnopharmacol. 2013; 148 (2): 433-40. [DOI:10.1016/j.jep.2013.04.032]
8. Kumar B, Vijayakumar M, Govindarajan R and Pushpangadan P. Ethnopharmacological approaches to wound healing-exploring medicinal plants of India. J. Ethnopharmacol. 2007; 114 (2): 103-13. [DOI:10.1016/j.jep.2007.08.010]
9. Bahramsoltani R, Farzaei MH and Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Arch. Dermatol. Res. 2014; 306 (7): 601-17. [DOI:10.1007/s00403-014-1474-6]
10. de Campos EP, Trombini LN, Rodrigues R, Portella DL, Werner AC, Ferraz MC and et al. Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents. BMC Res. Notes. 2015; 8 (1): 269. [DOI:10.1186/s13104-015-1251-4]
11. Loy CJ, Mahmood K and Saliou C. Compositions comprising Lilium candidum extracts and uses thereof. Google Patents; 2013. https://patents.google.com/patent/US8481093B2/en.
12. Kopaskova M, Hadjo L, Yankulova B, Jovtchev G, Galova E, Sevcovicova A and et al. Extract of Lillium candidum L. can modulate the genotoxicity of the antibiotic zeocin. Molecules 2012; 17 (1): 80-97. [DOI:10.3390/molecules17010080]
13. Eisenreichová E, Haladová M, Mučaji P and Grančai D. The study of constituents of Lilium candidum L. Acta Facult. Pharm. Univ. Comenianae 2004; 51: 27-37.
14. Patocka J, Navratilova Z and Yokozawa T. Bioactivity of Lilium candidum L-A Mini Review. Biomedical Journal of Scientific & Technical Res. 2019; 18 (5): 13859-62. [DOI:10.26717/BJSTR.2019.18.003204]
15. Nørbæk R and Kondo T. Anthocyanins from flowers of Lilium (Liliaceae). Phytochemistry 1999; 50 (7): 1181-4. [DOI:10.1016/S0031-9422(98)00661-X]
16. Usher G. A dictionary of plants used by man. 1974; 619 Seiten. Constable and Company Ltd., London.
17. Mucaji P, Haladova M, Eisenreichova E, Sersen F, Ubik K and Grancai D. Constituents of Lilium candidum L. and their antioxidative activity. Ceska a Slovenska Farmacie 2007; 56 (1): 27-9.
18. Mucaji P, Hudecova D, Haladova M and Eisenreichova E. Anti-yeast activity of ethanol extracts of Lilium candidum L. Ceska a Slovenska Farmacie 2002; 51 (6): 297-300.
19. VAckuková A, EsENREIcov E, HALAoov M, Muci P, Jóžová B and Novor L. Potential carcinogenic and inhibitory activity of compounds isolated from Lilium candidum L. Neoplasma. 2000; 47 (5): 313-8.
20. Bonjar S. Evaluation of antibacterial properties of some medicinal plants used in Iran. J. Ethnopharmacol. 2004; 94 (2): 301-5. [DOI:10.1016/j.jep.2004.06.007]
21. Picci V. The exploitation of medicinal plants of the Mediterranean area. J. Ethnopharmacol. 1980; 2 (1): 81-9. [DOI:10.1016/0378-8741(80)90035-5]
22. Francis JA, Rumbeiha W and Nair MG. Constituents in Easter lily flowers with medicinal activity. Life Sciences 2004; 76 (6): 671-83. [DOI:10.1016/j.lfs.2004.10.001]
23. Yarmolinsky L, Zaccai M, Ben-Shabat S, Mills D and Huleihel M. Antiviral activity of ethanol extracts of Ficus binjamina and Lilium candidum in vitro. N. Biotechnol. 2009; 26 (6): 307-13. [DOI:10.1016/j.nbt.2009.08.005]
24. Alsaad S and Mikhail M. Periocular hyperpigmentation: a review of etiology and current treatment options. J. Drugs Dermatol. 2013; 12 (2): 154-7.
25. Momtaz S, Hussein AA, Ostad SN, Abdollahi M and Lall N. Growth inhibition and induction of apoptosis in human cancerous HeLa cells by Maytenus procumbens. Food Chem. Toxicol. 2013; 51: 38-45. [DOI:10.1016/j.fct.2012.09.005]
26. Bahramsoltani R, Farzaei MH, Abdolghaffari AH, Rahimi R, Samadi N, Heidari M and et al. Evaluation of phytochemicals, antioxidant and burn wound healing activities of Cucurbita moschata Duchesne fruit peel. Iranian J. Basic Medical Sciences 2017; 20 (7): 798.
27. Olajire A and Azeez L. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. African J. Food Science and Technol. 2011; 2 (2): 22-9.
28. Goswami S, Kandhare A, Zanwar AA, Hegde MV, Bodhankar SL, Shinde S and et al. Oral l‐glutamine administration attenuated cutaneous wound healing in Wistar rats. Int. Wound J. 2016; 13 (1): 116-24. [DOI:10.1111/iwj.12246]
29. Hassanzadeh G, Hajmanouchehri F, Roi A, Hassanzadeh N, Shafigh N, Barzroudipour M and et al. Comparing effects of Silver sulfadiazine, Sucralfate and Brassica oleracea extract on burn wound healing. Life Science J. 2013; 10 (supp.): 104-13.
30. Budovsky A, Yarmolinsky L and Ben‐Shabat S. Effect of medicinal plants on wound healing. Wound Repair Regen. 2015; 23 (2): 171-83. [DOI:10.1111/wrr.12274]
31. Antunes-Ricardo M, Gutierrez-Uribe J and O Serna-Saldivar S. Anti-inflammatory glycosylated flavonoids as therapeutic agents for treatment of diabetes-impaired wounds. Curr. Top. Med. Chem. 2015; 15 (23): 2456-63. [DOI:10.2174/1568026615666150619141702]
32. Li W, Kandhare AD, Mukherjee AA and Bodhankar SL. Hesperidin, a plant flavonoid accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats: role of TGF-ß/Smads and Ang-1/Tie-2 signaling pathways. EXCLI J. 2018; 17: 399.
33. Lodhi S, Jain A, Jain AP, Pawar RS and Singhai AK. Effects of flavonoids from Martynia annua and Tephrosia purpurea on cutaneous wound healing. Avicenna J. Phytomed. 2016; 6 (5): 578.
34. Ïnan A, Şen M, Koca C, Akpinar A and Dener C. The effect of purified micronized flavonoid fraction on the healing of anastomoses in the colon in rats. Surg. Today 2006; 36 (9): 818-22. [DOI:10.1007/s00595-006-3251-4]
35. Gao J, Zhang T, Jin Z-Y, Xu X-M, Wang J-H, Zha X-Q and et al. Structural characterisation, physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb. Food Chem. 2015; 169: 430-8. [DOI:10.1016/j.foodchem.2014.08.016]
36. Jin L, Zhang Y, Yan L, Guo Y and Niu L. Phenolic compounds and antioxidant activity of bulb extracts of six Lilium species native to China. Molecules 2012; 17 (8): 9361-78. [DOI:10.3390/molecules17089361]
37. Kant V, Gopal A, Pathak NN, Kumar P, Tandan SK and Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int. Immunopharmacol. 2014; 20 (2): 322-30. [DOI:10.1016/j.intimp.2014.03.009]
38. Alvarez-Suarez JM, Giampieri F, Cordero M, Gasparrini M, Forbes-Hernández TY, Mazzoni L and et al. Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. J. Functional Foods 2016; 25: 38-49. [DOI:10.1016/j.jff.2016.05.008]

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