year 19, Issue 75 (9-2020)                   J. Med. Plants 2020, 19(75): 92-101 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Azari F, Vazirian M, Shams Ardekani M R, Sharifzadeh M, Sadati Lamardi S N. Hypoglycemic effect of Polygonatum orientale Desf. rhizome extract on diabetes induced by streptozotocin in rat model. J. Med. Plants 2020; 19 (75) :92-101
URL: http://jmp.ir/article-1-2611-en.html
Hypoglycemic effect of Polygonatum orientale Desf. rhizome extract on diabetes induced by streptozotocin in rat model
Faramarz Azari1 , Mahdi Vazirian2 , Mohammad Reza Shams Ardekani3 , Mohammad Sharifzadeh4 , Seyede Nargess Sadati Lamardi * 5
1- Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2- Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3- Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran , n_sadati@sina.tums.ac.ir
Abstract:   (3834 Views)
Background: Polygonatum orientale, an herbaceous and perennial plant from family Asparagaceae, is native to the forests of northern Iran. The hypoglycemic effects of other species of the Polygonatum have been proven. Objective: The aim of this study was to evaluate the hypoglycemic effect of P. orientale rhizome extract and its effects on the antioxidant enzymes in the plasma of the normal and streptozocin induced diabetic rats. Methods: 30 male rats were divided into 6 groups; healthy control, negative control, positive control (metformin, 500 mg/kg) and three groups of diabetic rats (200, 400 and 800 mg/kg) that received the ethanolic (70%) extract orally. After 28 days effects of the extract on blood glucose, superoxide dismutase (SOD) and catalase enzymes, as well as total antioxidant capacity of the plasma (by FRAP test) were investigated. Results: According to the results, the percentage of reduction in blood glucose in the groups of 400 (2%) and 800 (25%) mg/kg compared to the diabetic control group was significantly different, (P<0.05) and (P<0.001), respectively. The difference in blood glucose levels between 400 and 800 mg/kg and metformin was not significant (P>0.05). Also, 200 and 400 mg/kg of the extract reduced the amount of SOD in the plasma comparable to the normal group. Conclusion: It can be concluded that the ethanolic extract of rhizome of P. orientale exhibits a significant hypoglycemic effect that may be related to the presence of steroids, flavonoids and polysaccharides.
Keywords: Polygonatum orientale, Streptozocin, Hypoglycemic effect, Superoxide dismutase (SOD), Catalase
Full-Text [PDF 978 kb]   (1464 Downloads)    
Type of Study: Research | Subject: Traditional Pharmacy & Traditional Medicine
Received: 2019/07/31 | Accepted: 2019/09/28 | Published: 2020/09/6
References
1. Kasper D L, Fauci A, Hauser S, Longo D, Jameson J. Harrison's principles of internal medicine. 19th ed. McGraw-Hill Companies, New York. 2015.
2. Farng RS BM, Mhewedi F and Elbavoly SA. Antioxidant activity of spice, essential oils and linoleic acid oxidation in aqueous media. J. the American Oil Chemists Society 1989; 6 (6): 792-9. [DOI:10.1007/BF02653670]
3. Tiwari BK, Pandey KB, Abidi A and Rizvi SI. Markers of oxidative stress during diabetes mellitus. J. Biomarkers 2013; 1-8. http://dx.doi.org/10.1155/2013/378790. [DOI:10.1155/2013/378790]
4. Chelikani P, Fita I and Loewen P. Diversity of structures and properties among catalases. Cell. Mol. Life Sci. 2004; 61: 192-208. [DOI:10.1007/s00018-003-3206-5]
5. Goodsell D. Catalase. Molecule of the Mont.doi:10.2210/rcsb_pdb/mom_2004_9 from https://pdb101.rcsb.org/motm/57 [DOI:10.2210/rcsb_pdb/mom_2004_9]
6. Mozaffarian V. Identification of Medicinal and Aromatic Plants of Iran. Farhang Moaser, Tehran. 2012, pp: 631-32.
7. Li WL ZH, Bukuru J and De Kimpe J. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J. Ethnopharmacol. 2004; 92: 1-21. [DOI:10.1016/j.jep.2003.12.031]
8. Kato A and Miura T. Hypoglycemic action of the rhizomes of Polygonatum officinale in normal and diabetic mice. Planta medica. 1994; 60: 201-3. [DOI:10.1055/s-2006-959458]
9. Sadati Lamardi SN, Majidi Z, Alipour C, Farajzadeh S, Gohari A, Shafaroodi H, Vazirian M and Ostad SN. Antioxidant Potential, Hypoglycemic Effect and Safety of Ajuga chamaecistus Ging. ssp. tomentella (Boiss.) Rech. f. Aerial Parts. RJP. 2018; 5(4): 53-63.
10. Organization for Economic Co-operation and Development (OECD) Guideline for testing of chemicals. Acute oral toxicity-fixed dose procedure, 420. Adopted: 17 th December2001. [Accessed 2018] Available from: https://www.oecd.org/chemicalsafety/risk-assessment/1948378.pdf
11. Furman BL. Streptozotocin‐induced diabetic models in mice and rats. Curr. Protoc. Pharmacol. 2015; 1(5): 47. [DOI:10.1002/0471141755.ph0547s70]
12. Meng X-M, Ma X-X, Tian Y-L, Jiang Q, Wang L-L, Shi R, Dingl and Pang S-G. Metformin improves the glucose and lipid metabolism via influencing the level of serum total bile acids in rats with streptozotocin-induced type 2 diabetes mellitus. Eur. Rev. Med. Pharmacol Sci. 2017; 21: 2232-37.
13. Erejuwa OO, Sulaiman SA, Wahab MSA, Sirajudeen KNS, Salleh MSM and Gurtu S. Antioxidant protective effect of glibenclamide and metformin in combination with honey in pancreas of streptozotocin-induced diabetic rats. Int. J. Mol. Sci. 2010; 11: 2056-66. [DOI:10.3390/ijms11052056]
14. Çakatay U and Kayali R. The evaluation of altered redox status in plasma and mitochondria of acute and chronic diabetic rats. Clinical Biochem. 2006; 39: 907-12. [DOI:10.1016/j.clinbiochem.2006.05.005]
15. Dejian H, Boxin O and Ronald L. The chemistry behind antioxidant capacity assay. J. Agric. Food Chem. 2005; 53: 1841-56. [DOI:10.1021/jf030723c]
16. Benzie IF and Strain JJ. The ferric reducting ability of plasma as a masure of antioxidant power; the frap assay. Anal. Biochem. 1996; 239: 70-6. [DOI:10.1006/abio.1996.0292]
17. Madesh M and Balasubramanian KA. using MTT reduction by superoxide. Indian J. Biochem. Biophys. 1998; 35(3): 184-8.
18. Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105: 121-6. [DOI:10.1016/S0076-6879(84)05016-3]
19. Ahn M-J, Kim CY, Yoon K-D, Ryu MY, Cheong JH, Chin Y-W, and Kim J. Steroidal Saponins from the Rhizomes of Polygonatum sibiricum. J. Nat. Prod. 2006; 69: 360-4. [DOI:10.1021/np050394d]
20. Wang H, Zhang Y, Hong Y and Liu J. Effects of the Polygonatum sibiricum polysaccharides on blood glucose in mice and their initial mechanical study. J. Pediatric Pharmacy. 2002; 1: 006.
21. Liu L, Dong Q, Dong X-t, Fang J-n and Ding K. Structural investigation of two neutral polysaccharides isolated from rhizome of Polygonatum sibiricum. Carbohydrate Polymers 2007; 70: 304-9. [DOI:10.1016/j.carbpol.2007.04.012]
22. Kato A and Miura T. Hypoglycemic action of the rhizomes of Polygonatum officinale in normal and diabetic mice. Planta Medica. 1994; 60: 201-3. [DOI:10.1055/s-2006-959458]
23. Deng Y, He K, Ye X, Chen X, Huang J, Li X, Yuan L, Jin Y, Jin Q, Li P. Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects. J. Ethnopharmacol. 2012; 141: 228-33. [DOI:10.1016/j.jep.2012.02.023]
24. Kato A, Miura T and Fukunaga T. Effects of steroidal glycosides on blood glucose in normal and diabetic mice. Biol. Pharm. Bull. 1995; 18: 167-8. [DOI:10.1248/bpb.18.167]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb