year 17, Issue 66 (5-2018)                   J. Med. Plants 2018, 17(66): 145-155 | Back to browse issues page

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Taherian M, Maghsoudi H, Taherian R, Rastegar H. The Effect of Hydroalcoholic Extract of Echinops cephalotes L. on Expression of Inflammatory Factors in Chondrocytes and THP-1 Cells as a Model of Monocyte/Macrophage and Human Cartilage Cells in Osteoarthritis. J. Med. Plants 2018; 17 (66) :145-155
1- Food and Drug Research Institute, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran
2- Department of Biotechnology, Payamenour University, Tehran, Iran ,
3- Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Cosmetic Products Research Center, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran
Abstract:   (6012 Views)
Background: Osteoarthritis (OA) is one of the main causes of physical disability worldwide. Considering the complications of common treatments of OA, including non-steroidal anti-inflammatory drug (NSAIDs) and corticosteroids, establishment of new treatments is crucial.
Objective: This study aimed to explore the effect of Echinops cephalotes extract on the main inflammatory biomarkers in OA.
Methods: Hydroalcoholic extract of Echinops cephalotes, Ibuprofen and betamethasone were prepared to investigate their effects on inflammatory biomarkers. Human monocyte/macrophage (THP-1) cells and chondrocytes cells were used as a model of monocyte/macrophage and human cartilage cells in osteoarthritis. Lipopolysaccharide (LPS) was used to induce production of inflammatory cytokines in both cells. After RNA extraction and production of cDNA, RT_PCR & PCR were done. Then Real Time-PCR was used to investigate the amount of expression of proinflammatory genes.
Results: Echinops cephalotes extract reduced mRNA expression level of proinflammatory cytokines in the cells induced by LPS. Moreover, production of PGE2 and NO in in the LPS-induced THP-1 cells was reduced by this extract. Ibuprofen and betamethasone were more effective in reducing above inflammatory agents than the extract.
Conclusion: Echinops cephalotes extract can be used as a supplementary treatment option in osteoarthritis to reduce NSAIDs and corticosteroids dose in treatment of this disease.
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Type of Study: Research | Subject: Pharmacognosy & Pharmaceutics
Received: 2017/07/7 | Accepted: 2017/11/20 | Published: 2018/06/23

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