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:: Volume 4, Issue 64 And S11 ((زبان: انگلیسی) 2018) ::
JMP 2018, 4(64 And S11): 50-58 Back to browse issues page
The Study of Relative Expression of Key Genes of Thymol Biosynthesis Pathway in Thymus vulgaris cv. ‘Varico 3’ under Cold Stress Using Real-Time PCR
Sh Habibi 1, A Qaderi 2, F Fatehi 3
1- (M.Sc.) Department of Horticultural Science, Faculty of Agriculture, Lorestan University, Lorestan, Iran
2- (Ph.D.) Medicinal Plants Research center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- (Ph.D.) Faculty of Agriculture, Payame Noor University (PNU), Tehran, Iran
Abstract:   (71 Views)
Background: Thyme (Thymus vulgaris L.) is a valuable medicinal plant which has many secondary metabolites such as thymol and carvacrol.
Objective: This study was done to evaluation of cold stress effect on gene expression in thymol biosynthesis pathway including DXR، HMGR and TPS1.
Methods: The effect of cold stress in the time period of 0, 3, 12, 24 and 48 hours on gene expression in thymol biosynthesis pathway including DXR, HMGR and TPS1 was evaluated by RT-PCR. This study was done on base of complete randomized design (CRD) in Institute of Medicinal Plants, ACECR, Karaj, Iran.
Results: Results showed that the cold stress had significantly effect on expression of studied genes. The highest expression of HMGR gene was observed within 3 hours after cold treatment (1.57 times more than control treatment).  The expression of DXR and TPS1 genes were reduced in different periods of cold stress.
Conclusion: In general, the genes expression of thymol and carvacrol biosynthesis pathway including DXR, HMGR and TPS1 were significantly changed by cold stress.
Keywords: Thymus vulgaris L., Cold stress, Gene expression, Thymol
Full-Text [PDF 339 kb]   (36 Downloads)    
Type of Study: Research | Subject: Agriculture (medicinal plants)
Received: 2017/12/31 | Accepted: 2017/12/31 | Published: 2017/12/31
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Habibi S, Qaderi A, Fatehi F. The Study of Relative Expression of Key Genes of Thymol Biosynthesis Pathway in Thymus vulgaris cv. ‘Varico 3’ under Cold Stress Using Real-Time PCR. JMP. 2018; 4 (64) :50-58
URL: http://jmp.ir/article-1-1975-en.html
Volume 4, Issue 64 And S11 ((زبان: انگلیسی) 2018) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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