Background: Considering the fact that traditional knowledge forms the basis for development of new natural products in the other hands the elder people in some villages possess the traditional knowledge and with death of them, this knowledge is rapidly eroding. So it is an urgent necessity to record and conserving as quickly as possible all information about plants. Objective: Consideration and study of connections between usage and cultures believes about medicinal plants (Ethnobotany) is interesting and important basis for research. The aim of this research was studied of two family (Lamiaceae and Rosaceae) in some Alamut villages to know how the people use of these plants for their treatment. Methods: The investigated area was divided into different villages with the help of map. Questionnaires were used and devised to identify and document the traditional knowledge of local people. Information and data on various aspects of the plants such as traditional uses, and collection method and time of each species in the area were gathered from the local experienced persons through interviews and discussions. The plants were gathered from different altitude and sites. Then the fully dried specimens were mounted on herbarium sheets. A set of voucher specimens were deposited to IMPH. Results: In this investigation 11 medicinal species of Lamiaceae and 10 medicinal species of Rosaceae family were found that used frequently by native people. Conclusion: In this region there are some rare plants that can be subjected for investigations by researcher for their effects that may be interesting for their therapeutic effects.

Background: Ultrasonic assisted extraction (UAE) is a robust and efficient method as a desirable alternative to conventional strategies for extracting active ingredients from different parts of plants. Coupling this technique with hydrodistillation is an effective method for producing essential oil from plant material. Objective: In this study, we successfully combined ultrasonic technology with hydrodistillation (HD) and optimized the system to reduce the time of the isolation and increase the yield of the essential oil obtained from Stureja khuzistanica Jamzad (SK). Methods: In the next step, isolation time, yield, and quality of essential oils obtained by conventional and ultrasonic-assisted methods were compared. Ethyl acetate (EA) was used to recover the remaining essential oil in the distillate. Results: All oils and distillates were analyzed qualitatively and quantitatively via the GC-FID and GC-MS techniques. The minimum inhibitory concentration (MIC) of SK essential oil against Escherichia coli (0.5 mg/ml to 8.0 mg/ml) and Staphylococcus aureus (0.25 mg/ml to 8.0 mg/ml) were calculated. Conclusion: Our results show that while the obtained essential oils chemical profile and biological properties are comparable, this method can enhance essential oil isolation yield efficiency by up to 40 %.