Search published articles


Showing 2 results for In Vitro Culture
Evaluation Effect of Different Density Auxin and Fungi Piriformospora indica and Sebacina vermifera on Mentha piperita and Thymus vulgaris In vitro Condition
H Kari Dolatabadi , E Mohammadi Goltapeh , A Moini , A Varma ,
year 11, Issue 42 (5-2012)
Abstract

Background: Mentha piperita and Thymus vulgaris are two important species of family Lamiaceae that abundantly used in pharmaceutical, food, cosmetic and hygienic industries.

Objective: Objectives in this study were the effect of different concentration of auxin and fungi Piriformospora indica and Sebacina vermifera on the growth and development of peppermint and thyme in vitro condition. 

Methodes: Two distinct experiments were conducted the first evaluate the effect of different auxin levels (IAA ، NAA ، IBA) on M. piperita and T. vulgaris growth, and the second examined the effect of two fungi Piriformospora indica and Sebacina vermifera on plant height, root length, shoots and root weight in in vitro in a completely randomized design.

Results: The first study suggested the most effective hormone dose for the M . piperita growth was 1mg per liter of IBA and in the T. vulgaris it was 1 mg per liter of IAA. Increasing of the hormone doses resulted in growth decrease. The second study evolved that growth of the plants inoculated with fungi increased significantly. The data indicated that the plants inoculated with S. vermifera were the highest of all and the plants inoculated with P. indica had the most weight. The nodes of M. piperita and numbers of shoots in T. vulgaris increased significantly.

Conclusion: These results indicate that the best hormone for tissue culture peppermint and thyme are 1mg per liter of IBA and IAA, respectively. Also using these fungi can increase grow and development of plants.


Rapid Micropropagation of Lemon Verbena (Lippia citriodora L.) Using In vitro Culture
A Oladzad , A Qaderi , H Naghdi Badi , Ar Zare ,
year 11, Issue 42 (5-2012)
Abstract
Background: The genus Lippia contains more than 200 species that Lemon verbena (Lippia citriodora) has a high medicinal value. Therefore expansion of its acreage using development of rapid and new propagation methods has a high importance. Objective: The aim of this study was to present a rapid, new and effective method, using nodal segments, for large scale propagation of this plant. Methods: In order to the shoot micropropagation, lateral buds on nodal segments were placed on MS medium supplemented with different concentrations of auxin and cytokinin hormones. Root induction was studied in the media containing IBA, NAA and activated charcoal. For acclimatization, plants were transferred to plastic bags containing a mixture of vermiculite, perlite and soil. Results: The highest number of shoots was obtained on the MS medium supplemented with 3 mg/L BAP in combination with 0.1 mg/L IBA and well-developed shoots were rooted on the MS medium with 0.5 mg/L IBA and 2 gr/L activated charcoal. Conclusion: According to the high rate of proliferation in the media supplemented with BAP and IBA (3 and 0.1 mg/L, respectively), this technique can be used instead of conventional propagation methods, as a quick and economical technique to propagation of a wide range of the same plants.

Page 1 from 1     

© 2025 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb