Showing 11 results for Hypericum Perforatum
H Hosseinzadeh , Gr Karimi , M Rakhshanizadeh ,
year 3, Issue 10 (5-2004)
Abstract
We studied the anticonvulsant activity of the aqueous and ethanolic extracts of Hypericum perforatum aerial parts in mice. The pentylenetetrazole (PTZ) and the maximal electroshock seizure (MES) tests were used for assessing the anticonvulsive effects of this plant. In the PTZ test, the extracts (0.1-1 g/kg, i.p.) delayed the onset of tonic convulsions and protected mice against mortality. In the MES test, both extracts did not show antiseizure activity. L-NAME (1-10 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, reduced the anticonvulsant activity of the extracts. The results of this study indicate that the extracts of H. perforatum aerial parts could contribute to the control of petit mal seizure and this effect may be partially mediated by nitric oxide pathway.
H Naghdi Badi , Sa Ziai , Mh Mirjalili , M Ahvazi , F Khalighi Sigaroodi, B Habibi Khaniani , A Farahani ,
year 3, Issue 11 (9-2004)
Abstract
St John’s wort, Hypericum Perforatum L. (Culsiaceae) is an important medicinal plant, which has different bioactive constituents and hypericin (a naphtodianthrone) is one of this compounds. Hypericin has many pharmacological effects such as antidepressant, antiviral and antibacterial, which cause to high production and consumption. It has known that region and genetic (accession) have significant effects on yield and content of active constituents of this plant. Because of existing 5 accessions in gene bank of medicinal plants Institute –ACECR, this study has been conducted in order to determination the best accession and evaluation role of genetic and environment on quantitative yield and hypericin content. This study has been done in research farms of medicinal plants Institute - ACECR along 1999- 2002. The experimental design was a randomized complete block with a split-plot arrangement. The parameters are measured in this study included: dry and wet herbage, content of hypericin, plant height and diameter in flowering stage. Combined analysis of variance indicated that genetic (accession) had significant effect on wet herbage (p<0.05), dry herbage, plant height & diameter (p<0.01) but had not significant effect on hypericin content. Environmental conditions (years) affected on hypericin content (p<0.01). Therefore accession and environment have main roles in production of medicinal parts and hypericin content in this plants, respectively. They could be considered as two key factors for an economically successful cultivation.
M Pakgohar , M Ahmadi , Mh Salehi Surmaghi , A Mehran , Sh Akhondzadeh ,
year 4, Issue 15 (9-2005)
Abstract
Introduction: The premenstrual syndrome is characterised by Physical and Psychological symptoms apearing regularly during the week prior to menstruation and disappearing within a few days of its onset, causing disruption to occuptional, family and personal functioning. Anecdotal evidence suggests that Hypericum is useful in releving the symtoms of premenstrual syndrome, but as yet there has been no clinical investingation of this. Objective: To compare the efficacy of Hypericum with placebo to treat the symptoms of premenstrual syndrome. Methods: A double blind, placebo controlled, randomised design was used in the clinical study. 70 Students of Tehran University with premenstrual syndrome who were in otherwise good Physical and mental health, completed Daily symptom Ratings for two cycles. Then participants devided were in two groups (active 35, Placebo 35) and took Hypericum or placebo for two complete menstrual cycles (2 30 drops for 7 days before menstruation). Results: Hypericum obtained a mean reduction of %46.45, in symptoms and placebo %18.1. There were significant reductions in all symptoms between two groups. (p=0.000) Treatment was well tolerated and there was not any discontinuation or complication with Hypericum. Conclusion: Hypericum treats premenstrual syndrome, and offers a therapy limited to the luteal phase for at least two cycles.
M Larypoor , A Akhavansepahy , N Rahimifard , H Rashedi ,
year 8, Issue 31 (9-2009)
Abstract
Background: Dermatophytes are the main cause of human superficial mycosis that is still a public health problem especially in tropical countries such as Iran. Objective: The aim of this study was determining the antifungal effect of Hypercom perforatum essential oil. Methods: The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) for the essential oil of the plant Hypericum perforatum against various dermatophytes were determined. The essential oil of Hypericum perforatum was obtained by hydro distillation of the dried plant. Clinical isolates of dermatophytes (Epidermophyton floccosum, Microsporum canis, Microsporum gypseum, T. mentagrophytes var. interdigital, T. mentagrophytes var. mentagrophytes., T. rubrum and Trichophyton tonsurans) were used for determining antifungal activity of this essential oil by in vitro tube dilution technique. Results: MIC90 and MFC90 values were remarkable. T. mentagrophytes var. interdigital showed a>1 log10 difference in viable count between treatment and control within the first hour, whereas E. floccosum did not. The essential oil of H. perforatum sufficiently inhibited and killed all tested dermatophytes in all different dilutions. The changes in growth curve of the treated dermatophytes were significant compared with the untreated dermatophytes. Conclusion: Terpinen-4-ol is the main component of the essential oil of H. perforatum, and perhaps could play the important role in antidermatophyte activity among the other components. It is suggested trying the in-vivo effects of Hypericum perforatum ointment or its other medicinal forms in the treatment and controlling of dermatophytes infections.
A Riazi, N Majnoun Hosseini , H Naghdi Badi , Mr Naghavi , Sh Rezazadeh , Y Ajani ,
year 10, Issue 39 (9-2011)
Abstract
Background: St. John’s wort (Hypericum perforatum L.) is an important medicinal plant that has been widely used for its antidepressant properties. Objective: In this study morphological characteristics variation of populations of H. perforatum were investigated. Methods: In this study, different morphological characteristics of 25 H. perforatum populations in Iran’s natural habitats were evaluated in 2010. Results: The results indicated that the highest coefficients of variations were found in flowers number, the fifth internodes’ length and capsule length (respectively, 93.46, 85.28 and 84.7%) however the lowest coefficients of variations were seen in flower width (11.72%) and sepal length (11.91 %). The largest dimensions of flowers, sepals and petals related to the population which was gathered from Zanjan/Tarum city. The highest leaves dimensions and dark glands density on the bottom leaves were observed in Alamut and Kalaleh populations. The highest positive significant correlations were seen between flower length with flower width dark glands density on the middle leaves surface area with dark glands density on the bottom leaves surface area petal length with flower length and width capsule length with capsule width non-flowering branches number with branches number light glands density on the middle leaves surface area with light glands density on the bottom leaves surface area. The lowest significant correlations were observed between capsule length with flower length and width. The principal components analysis showed that four components explained 68.9 % of total variance. The cluster analysis divided these populations into four clusters with no consistency in their geographical distributions. Conclusion: In conclusion, this study indicated that there were high variations among the Iranian St. John’s wort populations which can be utilized in the breeding programs.
Mr Morshedloo , A Ebadi , Mr Fatahi Moghaddam , D Yazdani ,
year 11, Issue 41 (2-2012)
Abstract
Background: St. John’s Wort (Hypericum perforatum L.), as the most important species of the genus, has been used in traditional medicine for thousands of years. Recently, the plant compounds are used to treat mild and moderate depression around the world.
Objective: This study identified the constituents of essential oil and total phenolic compounds and also antioxidant activity of methanol extract of H. perforatum collected from wildly growing area in the north of Iran (Tonecabon, Mazandaran province) .
Methods: Essential oil was extracted by hydro-distillation of air dried flowering shoots using Clevenger apparatus. The essential oils were analyzed by both gas chromatography (GC) and gas chromatography −mass spectrometry (GC-MS). Total phenolic compounds in the methanol (80%) extract were determined according to the Folin-Ciocalteu reagent using tannic acid as standard. Antioxidant activity of extract was also evaluated by DPPH free radical method.
Results: Monoterpens (25.63%) were found to be the major fraction of the oil and α- Pinene (21.88%) was found to be the most abundant compound, followed by nonane (9.77%), n- octane (9.13%) and dodecanol (6.80%). In addition, the results indicated total phenol content (50 μg gallic acid equivalent/g) and antioxidant activity (34. 37 μg/ml) of methanolic extract.
Conclusion: α - Pinene was the major constituent of essential oil of H. perforatum tested sample and its methanol extract contained the high amount of total phenolic compounds and consequently exhibited a considerable antioxidant activity.
Mr Morshedloo , A Ebadi , Mr Fatahi Moghaddam , D Yazdani,
year 11, Issue 42 (5-2012)
Abstract
Background: Essential oils contain a large variety of volatile secondary metabolites such as terpenes, terpenoides, phenolic and aliphatic derivative. Nowadays, These compounds extensively used in Pharmaceutical , sanitary and cosmetic industries. Antimicrobial activity of these oils and their components such as α-pinene and β-pinene, the main essential oil constituent of some Hypericum spp., have been reported.
Objective: This study was conducted to identify the constituents of essential oil in three Hypericum species in Iran to compare differences among their major volatile constituents.
Methods: Essential oils were extracted by hydro-distillation of fresh plant materials using Clevenger apparatus. The essential oils were analyzed by both gas chromatography (GC) and gas chromatography −mass spectrometry (GC-MS).
Results: Monoterpens were found as the major constituent of essential oil in H. perforatum L. and H. patulum Thunb . However, n-alkans were the major constituent of the oil in H. tetrapterum Frise . In this study, α-Pinene (21.88%) was found to be the most abundant compound in the H. perforatum whilst β- Pinene (30.20%) and n-undecane (30.84%) were the main constituents of H. patulum and H. tetrapterum essential oils respectively.
Conclusion: In this study, we found that the different plant species made a remarkable difference in the essential oil constituents. In this study the most component of essential oils of H. perforatum and H. patulum were α-Pinene and β-Pinene respectively and this component can be make them promising for treatment of infectious diseases such as infectious endocarditis.
L Rezaei , A Qaderi , Mr Naghavi , Ma Ebrahimi, As Riazi , A Mehrafarin , H Naghdi Badi ,
year 11, Issue 44 (11-2012)
Abstract
Background: Hypericum perforatum is one of the valuable medicinal plants in Iran that is used in treating human diseases likes mania, anxiety and depression. Objective: Iranian H. perforatum populations were gathered from deferent region of Iran and also their genetic diversity in company with the possible relationship between genetic diversity and geographical distribution were studied. Methods: DNA was isolated by CTAB method from young leaves and double digested by EcoRI and Tru1I enzymes. Polymorphic DNA markers generated by DNA fingerprinting technique AFLP (Vos method) using 12 primers combinations. DNA fragments detected with silver nitrate staining according to Basam protocol. Results: Totally 235 bands were scored, that 97% of them were polymorphic. The PIC values ranged between 0.31 and 0.45 with mean of 0.38. The highest and the lowest levels of genetic similarity were 0.89 and 0.29, respectively. Cluster analysis using UPGMA method and DICE similarity coefficient indicated a high genetic diversity among H. perforatum populations. There was no relationship between genetic diversity and geographical distribution. Also for the all loci, the PC1 and PC2 explained 12.8% and 8.3% of the variation, respectively. Conclusion: Wide genetic diversity between Iranian H. perforatum pupulations provide applied information to performance of breeding programs and genetic resource management. Of course, investigation of amount of hypericin and hyperforin metabolites in these populations are requiring to selection paramount genotypes.
M Montazeri M (m.sc.), M Azadbakht , H Najafi Zarrini , Gha Nematzadeh, A Pakdin Parizi , A Davoodi,
year 17, Issue 67 (9-2018)
Abstract
Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites.
Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Finally, the hypericin have been producted by the hairy roots.
Methods: First, the seeds have been incubated in the plant media to hairy roots produced. Then, the hairy roots have been dipped in Rhizobium rizogenes suspension for biotransformation of bacterial genes. Morphological and phytochemical features of hairy roots have been determined in order to select the H. perforatum genotypes with higher hypericin contents. The fresh and dry weight of ten lines clones were measured after 30 days. In addition to, methanolic extracts of final hairy roots have been prepared and hypericin has been isolated and assayed by High Performance Liquid Chromatography.
Results: According to the observations, one of ten hairy root lines shows 75-fold higher hypericin content (339.27 ppm) compared to non-transformed H. perforatum (4.58 ppm) in the same of body weight of roots. The clone with the most content of hypericin had significant development of biomass of hairy roots and increase the hypericin production. Transformed clones were varied in morphology, growth, and metabolite productivity.
Conclusion: The mentioned methods induce the production of hairy root secondary metabolites in high scale to improve the quality and the quantity of pharmaceutical compounds.
Mahmonir Rezaei Nazari, Vahid Abdossi, Fariba Zamani Hargalani, Kambiz Larijani,
year 21, Issue 81 (3-2022)
Abstract
Background: Hypericum perforatum L. belongs to the Hypericaceae family has been considered due to its medicinal properties. The use of nanofertilizers can improve the yield and medicinal value of plants. Selenium has a protective role and a positive effect on the quantitative and qualitative characteristics of plants. Objective: Due to the importance of secondary metabolites of this plant and its economic value as well as the use of environmentally friendly fertilizers, this study was performed in the greenhouse of Shariati University of Tehran in 2019 in the form of a randomized complete block design with 3 replications. Methods: Nano selenium and selenate selenium were applied at concentrations of 6, 8, 10 and 12 mg/L in the rosette stage. Essential oil components were identified using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Results: The results showed that the highest amount of α-pinene with 22 % was obtained in the control treatment and also in the treatments of 6 and 8 mg/L sodium selenate with 21.6 % and 19.5 %. The maximum content of n-octane was in the foliar application of 12 mg/L nano selenium with 16 %. Maximum nonane was obtained with 18 % and 16 % of 12 and 10 mg/L sodium selenate, respectively. Conclusion: Sodium selenate treatments are recommended if the purpose is producing normal hydrocarbon compounds.
Farzaneh Rezaeinejad, Hasan Bardania, Farideh Ghalamfarsa, Sajad Hasanzadeh, Farhad Jadidi-Niaragh, Ghasem Ghalamfarsa,
year 21, Issue 82 (5-2022)
Abstract
Background: Colorectal cancer (CRC) continues to be a leading cause of cancer related death in the world and approximately 70 to 75 % of patients with metastatic colorectal cancer survive for up to 1 year after diagnosis. Curcumin (CUR) is a potential chemotherapeutic agent used to treat cancer. There is ample evidence of the inhibitory effects of Hypericum perforatum L. extract (HPE) on cell proliferation and its effects on the induction of apoptosis in various human cancer cell lines. Objective: The purpose of this study was to investigate the proapoptotic effect of HPE and its nanoliposomes (HPE-Lip) and to scrutinize the synergistic and therapeutic potential of HPE/CUR-loaded nanoliposome (HPE/CUR-Lip). Methods: In the present in vitro study, SW1116 and SW48 cell lines were cultured and then treated with different doses of HPE, CUR, bare liposome solely (Lip-Sol), and nanoliposomes loaded with HPE (HPE-Lip), CUR (CUR-Lip) and CUR/HPE (HPE/CUR-Lip) for 24, 48 and 72 hours. Cytotoxicity was measured by MTT assay and apoptosis rate by an annexin-V FITC/propidium iodide double-staining method using flow cytometry. Results: The results showed that cell viability was inhibited in a dose-dependent and time-dependent manner in all groups compared to the control group. The use of nanoliposomes improved the outcomes. HPE/CUR-Lip exhibited higher in vitro cytotoxic and proapoptotic activity against SW1116 and SW48 cell lines (P < 0.05). Conclusion: The findings of this study suggest that the HPE/CUR-Lip complex could provide a potential strategy to achieve a synergistic effect of HPE and CUR in the treatment of colorectal cancer.
