Showing 10 results for Glycyrrhiza Glabra
H Hajimehdipoor, Y Amanzadeh, T Hasanloo, M Shekarchi , Z Abedi , M Pirali Hamedani ,
year 7, Issue 27 (8-2008)
Abstract
Background: Glycyrrhiza glabra L. has been used in respiratory diseases, gastric and duodenal ulcers and liver insufficiencies for many years. The plant grows in different regions of Iran. Since our country is considered one of the greatest exporting countries of the plant roots, investigation of licorice quality from different parts of Iran and determination of the best area for its growing seems to be important. Objective: According to validated references, glycyrrhizic acid percentage and water-soluble extractives are determinative of quality therefore, in this investigation, these two factors have been measured in the plants collected from different regions of Iran. Method: Roots of the plant were collected from Kerman, Kermanshah, Sirjan, Sarhadd (Fars province), Istahban (Fars province), Ghasredasht (Fars province), Najaf Abad (Isfahan province), Ganjnameh (Hamedan province), Ekbatan (Hamedan province), Mahabad, Khorram Abad and Ardabil and water-soluble extractives using maceration method and glycyrrhizic acid percentage with HPLC technique were determined in each sample. Results: The results showed that the amount of water-soluble extractives and glycyrrhizic acid are high in samples from Kermanshah, Sarhadd and Kerman while these two factors are the lowest in the plant roots collected from Ekbatan and Ganjnameh. Conclusion: The plant roots collected from Kermanshah, Sarhadd and Kerman have the highest quality and are the best for medicinal purposes, sweetening and flavoring agent and for exporting as well.
Sh Shoeibi , H Hajimehdipoor, N Rahimifard , Sh Rezazadeh , T Hasanloo , F Bagheri, A Amini ,
year 9, Issue 36 (12-2010)
Abstract
Background: Helicobacter pylori infection is common in gastrointestinal tract and lead to gastritis, peptic ulcer and finally stomach cancer. Many synthetic drugs are used to eradicate the bacteria but there are some problems in treatment due to drug resistance and frequent relapses of infection. Herbal drugs are very important in the case. Glycyrrhiza glabra is used for treatment of H. pylori infection. This plant grows in different regions of Iran, therefore, investigation of anti H. pylori effects of the plant collected from different growing area is important. Objective: The aim of this study is determination of MIC of licorice roots collected from different regions of Iran against H. pylori in order to introduce the best growing area of the plant. Methods: Roots of the plant were collected from Kerman, Kermanshah, Sirjan, Sarhadd (Fars province), Istahban (Fars province), Ghasredasht (Fars province), Najaf Abad (Isfahan province), Ganjnameh (Hamedan province), Ekbatan (Hamedan province), Mahabad, Khorram Abad and Ardabil and extracted by using ethanol 80%. MIC of each extract was determined against three clinical isolated strains of H. pylori which obtained during endoscopy procedure. Results: The results showed that susceptibilities of three clinical isolated bacteria to the licorice extracts are different. Among the studied extracts, licorice roots collected from Ardabil showed the lowest MIC (at least: 125 ppm) and all strains were almost resistant to the extract from Mahabad. Conclusion: It is concluded that drug resistance is observed in herbals just like synthetic drugs. Moreover, in order to achieve best therapeutic results, before using an herbal preparation for treatment a disorder, growing area of a medicinal herb should be considered.
H Oloumi , N Hassibi ,
year 11, Issue 42 (5-2012)
Abstract
Background: Glycyrrhiza glabra roots and rhizomes which are extensively used in herbal medicines for them health effects containing more than 100 various useful compounds including phenolics and triterpene saponins (glycyrrizin) grow as a weedy plants in many different localities of Kerman province.
Objective: Regards to application of licorice roots in pharmaceutical industry, the mail goal was the comparison of some biochemical compounds content important in pharmacy, food industry and economics in G. glabra root gathering from seven localities of Kerman province.
Methods: In this research project, the content of total phenolic compounds, flavonoids and other UV absorbent compounds, tannins, anthocyanins and glycyrrhizin gathered from some natural localities of Kerman province including Baft, Bardsir, Rayen, Zangiabad and Koohbanan was investigated.
Results: The highest content of glycyrrhizin was found in Andohjerd. Shahrbabak locality showed the highest content of total phenolic compounds while the lowest was found in Andohjerd. Tannins content were highest in roots gathered from Baft.
Conclusion: Based on the results, in seems that the profile of secondary metabolites differs depending on climate parameters and environmental conditions. Therefore, roots gathered from Andohjerd with lowest height show higher quality of regards to glycyrrhizin production and roots form localities with more height like Rayen and Baft have more total phenolic compounds.
M Khanahmadi M, H Naghdi Badi , S Akhondzadeh , F Khalighi – Sigaroodi , A Mehrafarin , S Shahriari , R Hajiaghaee ,
year 12, Issue 46 (5-2013)
Abstract
Glycyrrhiza glabra L (Licorice) as native and pasture medicinal plant has most important export rule. Glycyrrhizic acid (GA) or Glycyrrhizin as a main active component of licorice root and rhizomes, is a triterpenoid saponin claimed to be up to 30-50 times sweeter than sucrose and to be used in pharmaceuticals, food and tobacco industry. Licorice root is a traditional medicine used mainly for the treatment of peptic ulcer, hepatitis C, and pulmonary and skin diseases, although clinical and experimental studies suggest that it has several other useful pharmacological properties such as antiinflammatory, antiviral, antimicrobial, antioxidative, anticancer activities, immunomodulatory, hepatoprotective and cardioprotective effects. A large number of components have been isolated from licorice, including triterpene saponins, flavonoids, isoflavonoids and chalcones, with glycyrrhizic acid normally being considered to be the main biologically active component. Understanding of the limitations and challenges in the medicinal plant are need for planning principles for development activities and conservation in this field.In this review summarizes the phytochemical, pharmacological and pharmacokinetics data, together with the clinical as one of the most important herbs in exports and adverse effects of licorice.
M Khanahmadi, H Naghdi Badi, S Akhondzadeh, F Khalighi–sigaroodi, A Mehrafarin, S Shahriari, R Hajiaghaee,
year 13, Issue 50 (5-2014)
Abstract
Glycyrrhiza glabra (Licorice) is a perennial shrub belongs to the Fabaceae family. It is widely used in food pharmaceutical products and tobacco industry because of its important secondary metabolites. Due to worldwide uses of licorice, it has significant role in export. One of the main bioactive compounds of the root is glycyrrhizic acid (GA) which is a saponine containing three carboxyl and five hydroxyl groups. Glycyrrhizic acid is used as an anti-inflammatory and anti-allergic remedy for the treatment of bronchial asthma, eczemas and other diseases. There are different methods for separation, purification, and analysis of glycyrrhizic acid (GA) from licorice roots. Most accepted technologies of them include using of solvents, water separation, polymeric macro porous resin and non-ionic surfactants. Also, high-performance liquid chromatography technique is the most important in analysis of GA.
A Hamta , Sma Shariatzadeh, M Soleimani Mehranjani , H Fallah Huseini, F Hosseinabadi,
year 13, Issue 50 (5-2014)
Abstract
Background: Glycyrrhiza glabra L. (G. glabra) root has been used in traditional medicine for treatment of several diseases. The main active constituent of G. glabra is glycyrrhizic acid with antioxidant property. The cytotoxic effects of several compound isolated from different plants have been attributed to their antioxidant properties. Objective: The present work was aimed to investigate the in-vitro cytotoxic screening of G. glabra root extract against 4T1 cell line derived from BALB/c mice mammary tumors. Methods: 4T1 cells were cultured in RPMI-1640 medium with 10% FBS and penicillin/streptomycin. Then cells treated with different concentration of G. glabra extract (50, 100, 200, 400, 800 µg/ml), taxol (1.25, 2.5, 5, 10, 20 nM) alone and in combination G. glabra and taxol for 24, 48, 72 hrs. Viability of the cells was measured through trypanblue and MTT staining. The cells morphology was studied using fluorescent dye. Results: G. glabra root extract and taxol showed significant cytotoxic effects on breast cancer cells. Condensation and deformation of the nuclei were also observed similarly for both treatments. Moreover in combination therapy, G. glabra extract enhances taxol induced cytotoxicity in cancerous cells. Conclusion: G. glabra root extract and taxol showed cytotoxicity effects and morphological changes in 4T1 cells. This reduction in the viability of the cells was dependent on dose and time.
M Farokhipour, Aa Bahaoddini, E Khoshnam,
year 15, Issue 60 (10-2016)
Abstract
Background: The licorice (Glycyrrhiza glabra) rhizome has been widely used in traditional medicine for treatment of gastrointestinal diseases.
Objective: In the present study, the effect of hydro-alcoholic extract of licorice rhizome on mechanical activity of isolated ileum of male rats has been studied.
Methods: Seven adult male rats were anesthetized by ethyl ether, their abdomen opened, and ileum dissected and divided into 1 cm segments. The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution (37 °C, pH=7.4). Their mechanical activity of ileum was recorded by power lab AD instrument in basal condition, and after administration of L-NAME (10-4M) drug in the presence and absence of licorice rhizome extract (0.0175 mg/ml). Also the mechanical activity of control group segments were recorded at the same condition with extract solvent (ethanol %70).
Results: A significant decrease in mechanical activity of the isolated ileum occurred after administration of hydro-alcoholic extract of licorice compared to the control group, while the mechanical activity of ileum tissue not significantly changed in the coadministration of L-NAME and extract between experimental and control groups.
Conclusion: We can conclude that hydro-alcoholic extract of licorice has modifying effect on ileum motility, and this activity may be occurred independently to the nitrergic system.
Ar Zebarjadi, M Safari, K Cheghamirza,
year 16, Issue 61 (1-2017)
Abstract
Background: Licorice, Glycyrrhiza glabra (belong to Leguminosae family) is one of the most popular medicinal plants in the world and it is widely used in many fields such as medical, pharmaceutical, confectionery and health industries. Different parts of licorice (shoots, leaves and roots) were had various components such as Glycyrrhzin that was used for some proposes.
Objective: The current study was done with the aim of gene transfer via Agrobacterium rhizogenes by ex vitro method for hairy root production in licorice.
Methods: The experiment was laid out as a completely randomized design (CRD) with five treatments in three replications. At first, root of young plantlets was eliminated and excited plantlets were putted in the glass wool contain suspension of bacteria. After 10 to 14 days of inoculation with Agrobacterium rhizogenes, the roots were appeared. The percentage of root induction by four strains of Agrobacterium (ATCC 15834, GMI 9534, A4 and A13) with check (without bacteria) was investigated.
Results: The results of PCR analysis with specific primers for roots of composite plants (putative transgenic) was shown that three strains of bacteria (A4, A13 and GMI 9534) and strain ATCC 15834, were produced 100% and 66.66% transgenic roots respectively.
Conclusion: Thus, production of composite licorice plants was remarked due to it has low cost, fast and simple.
Shima Sheikhi, Ali Khamesipour, Tayebeh Radjabian, Zahra Mojallal Tabatabaei, Tooba Ghazanfari,
year 19, Issue 74 (6-2020)
Abstract
Background: Cutaneous leishmaniasis by different species of Leishmania. Pentavalen antimonials as a first line drug for treatment of cutaneous leishmaniasis have several limitations and side effects. Natural products are more considered due to their less toxicity and cost, more efficient, safety and readily available antileishmania agents. Objective: The aim of this study was to explore the effect of hydroalcoholic extract of Glycyrrhiza glabra (licorice) roots and its main component glycyrrhizic acid on promastigote and amastigote of L. major. Methods: Different dosages of hydroalcoholic extract of licorice root, glycyrrhizic acid and Glucantime with promastigote of L. major were incubated at 26 °C for 24 and 48 hours then the percentages of alive promastigotes were measured by MTT assay. The antiamastigote effects of these drugs was examined by microscopic counting of the number of amastigotes in macrophages 24 hours after treating the parasite infected macrophages with them. Promastigote and infected macrophages without any treatment was used as negative controls. Results: The IC50 values of hydroalcoholic extracts of licorice root, glycyrrhizic acid and Glucantime on promastigote of L. major was 1250 ± 0.018, 3000 ± 0.017 and 50 ± 0.043 μg/ml after 24 hours, and 1000 ± 0.016, 3000 ± 0.017 and 25 ± 0.009 μg/ml after 48 hours, respectively. The IC50 values of the licorice root extract, glycyrrhizic acid and Glucantime on amostigate was 500, 1000 and 25 μg/ml, respectively. Conclusion: Hydroalcoholic extract of licorice root and glycyrrhizic acid had cytotoxic effects on promastigotes and amastigotes of L. major.
Maryam Allahdou, Mansour Omidi, Mohammad Reza Bihamta, Ali Reza Abbasi, Barat Ali Fakheri,
year 21, Issue 84 (12-2022)
Abstract
Background: Cytochrome P450s have essential roles in oxidative reactions during the biosynthesis of secondary metabolites, such as terpenoids. Objective: This research was aimed to identify the gene CYP72A154 as a gene involved in the glycyrrhizin biosynthesis pathway in Iranian licorice. Methods: CYP72A154 gene was isolated from Iranian licorice and cloned it into the PTG19-T vector. After confirmation of fragment length, the recombination plasmid was sent for sequencing. NCBI BLAST was used to analyze the nucleotide/ protein sequence homology between Glycyrrhiza glabra and other plants. The characterization of predicted amino acid sequences such as sequence homology, protein domains and functional sites, was performed using InterProscan. RT-PCR was performed to improve the relative expression this gene in the licorice root. Results: The query length was 314 aa, which after blasting in NCBI had about 78 to 80 % identity to the cytochrome P450 72A154 and 11-oxo-beta-amyrin 30-oxidase in species of G. glabra, G. uralensis and G. pallidiflora, as well as about 64 to 67 % with other species of the Fabaceae family. TMHMM analysis indicated the Exp number of AAs in TMHs was 22.11931, Exp number and first 60 AAs was 20.013. The results of RT-PCR revealed that the expression of this gene was comparable to the β-AS gene and CYP88D6, both being involved in the glycyrrhizin biosynthesis pathway. Conclusion: According to bioinformatics analysis and RT-PCR, it can be stated that the desired fragment belongs to the CYP72A154 gene and is also involved in the biosynthesis of glycyrrhizin.
