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Showing 7 results for Gas Chromatography

M Mosaddegh , M Kamalinejad , A Dehmaoobad Sharifabadi , B Esfahani ,
year 3, Issue 11 (9-2004)
Abstract

Composition of the volatile oils of the C. bigaradia, C. limon and C. delicosa have been investigated. The plant materials (leaves) were collected in spring 2000 from Corta (The medicinal plants garden) near Tonecabon, North of Iran. The oils were collected by means of hydrodistilation procedure. The yields were 1.4% v/w, 1.1 % v/w and 1% v/w respectively. Subesequently the oils were analyzed by gas chromatography mass spectrometry. The main constituents of C.bigaradia were linallol (38.72%), linalyl acetare (36.52%), α-terpineol (8.68%), of C. limon were geranial (31.45%), neral (23.16%), β-pinene (22.39%) and limonenc (6.7%) and of C. deliciosa were sabinene (37.64%), linallol (36.60%), δ-3-carene (4.42%) and citronellal (3.81%).

B Uma , K Prabhakar , S Rajendran, Y Lakshmi Sarayu ,
year 8, Issue 31 (9-2009)
Abstract

Background: Infectious diarrhoea is an emergent problem in both developing and developed countries for high rates of mortality in infants. In recent years, drug resistance to microbial pathogens has been commonly reported from all over the world. Medicinal plants represent a rich source of antimicrobial agents. Plants are used medicinally in different countries especially in India and are a source of many potent and powerful drugs. This study was aimed to explore the bioactive antimicrobial compounds present in Cinnamon zeylanicum, which is used as a spice in Indian foods. Objective: The aim of this study was to carry out Gas chromatography and Mass spectroscopy studies and to determine the antimicrobial activity of Cinnamon zeylanicum extract against some common microbial pathogens isolated from diarrhoeal patients. Methods: The extract was prepared by the method of maceration using methanol as extraction solvent. The antimicrobial activity was performed by disc diffusion method at the concentration 200, 100, 50, 25 and 10 mg/ml against E. coli, Salmonella typhi, Salmonella typhimurium, Shigella dysenterae, Shigella flexneri, Pseudomonas aeruginosa and Klebsiella pneumonia and the fungi Candida albicans, Candida tropicalis, Candida krusei which are isolated from the paediatric diarrhoeal samples. Gas chromatography and Mass spectroscopy studies were performed to identify the bioactive compounds. Results: The methanol extract was found very sensitive against all the isolated organisms. The preliminary phytochemical screening shows the presence of carbohydrate, glycosides, phenolic compounds, tannins, terpenes and proteins. The GC/MS study on the active principles shows the presence of 38 components. Conclusion: This study shows that the Cinnamon zeylanicum extract exhibit high antifungal activity as compared with antibacterial activity. The most identified compound by GC/MS to have antimicrobial property were monoterpenes, sesquiterpenes, aromatic aldehydes and ketones. Cinnamaldehyde was the major compound responsible for the antimicrobial activity.

Z Talebpour , S Najafi , A Sonboli , M Firozy , M Khosroshahi ,
year 12, Issue 48 (12-2013)
Abstract

Background: Tanacetum sonbolii Mozaff. is an endemic species of Tanacetum growing in Iran, west Azerbaijan. The composition of its essential oil can be varied due to conditions of selected extraction method. Thus, the selection of suitable extraction method is very effective to obtaine essential oil in native state. Objective: The purpose of this study is to apply head space sorptive extraction method at ambient and high temperature condition for essential oil extraction of Tanacetum sonbolii Mozaff. Methods: The compositions of extracted essential oils were investigated using gas chromatography-mass spectrometry. Also, solvent, temperature and time of desorption step and time of sorption step that influence on extraction efficiency were optimized. Results: Results indicated that temperature of extraction method had significant effects on essential oil content. Using hydrodistillation method, 26 components, representing 96.5% of the oil were indentified but only 6 compounds (95.6% of the oil) were found in essential oil using HS-SE at real condition (23 oC). Comparison of the major components percent levels of essential oils were shown that 81% of HS-SE essential oil is β- and α-pinene. While using hydrodistillation method, about 50% of essential oil is sesquirerpenes such as α-cadinol and globulol. Conclusion: The sampling of essential oils at 23oC by HS-SE compared to conventional extraction methods at high temperature (such as hydrodistillation) caused to have different extractions results.

M Mehran, H Hosseini, Ar Hatami, M Taghizade, Ar Safaei,
year 15, Issue 58 (4-2016)
Abstract

Introduction: Thyme is one of the mint family geniuses that belongs to Nepetoideae subfamily. This plant has been known as an antiseptic, antispasmodic and antitussive. Thyme essential oil has many properties that one of which is antioxidant activity. Objective: The aim of this study was the investigation of seven thyme oils and the comparison of their antioxidant activities. In this paper, seven species of essential oils of thyme from different regions have been collected and dried. Methods: The essential oil was extracted by using a Clevenger-type apparatus as British pharmaceutical for four hours. Obtained essential oils were analyzed by gas chromatography–mass spectroscopy (GC-MS) technique. Results: Different compounds were identified in the essential oils with different percentages. The main compound in Thymus daenensis and Thymus vulgaris was thymol with percentage of 43.8 for the former and 45.1 for the latter. While the dominate compound in Thymus migricus, Thymus eriocalyx, Thymus serpylum, Zataria multiflora and Thymus kotschyanus are linalool (41.8%), geraniol (61.8%), para cymene (23.8%), carvacrol (57.7%), and Pulegone (37.2%), respectively. Antioxidant activity of essential oils was measured by 2,2-diphenyl-1-picrylhydrazyl )DPPH) radical scavenging test. Conclusions: Antioxidant activities of Thymus vulgaris, Thymus daenensis, and Zataria multiflora were higher than other essential oils.


Sh Amini , F Tajabadi , M Khani , Mr Labbafi , M Tavakoli ,
year 17, Issue 67 (9-2018)
Abstract

Background: The Apiaceae family is defined with the diversity of essential oil. Some of composition could be used to control of storage pests.
Objective: This study was conducted to estimate and identification of the seed essential oil composition of four Apiaceae species.
Methods: The essential oil composition of the seeds of the four Apiaceae’s species including (Pimpinella anisum L., Foeniculum vulgar Mill, Coriandrum sativum L. and Petroselinum hortense) were identified by Gas chromatography- mass spectrometry (GC-MS) and compared with each other. Finally, biological effects of the four different species of Apiaceae family were evaluated against adult insects of two important storage pests.
Results: The results of analysis showed Anethol with 76.56% and 76.22% is the original component of Pimpinella anisum L., Foeniculum vulgar Mill, respectively. Also, Linalool with 58.80% and α-Pinene with 42.15% are the original components of Coriandrum sativum L. and Petroselinum hortense, respectively.
Discussion: Regarding to the identified components of the essential oils and obtained results, Pimpinella anisum L. and Coriandrum sativum L. showed the most fumigant toxicity on the storage pests.
 

Shahrzad Salari, Mehrdad Shamsaddini,
year 19, Issue 74 (6-2020)
Abstract

Background: Ducrosia anethifolia (DC.) Boiss is native to Iran and its essential oil has cytotoxic effects. Objective: Herbal Essences have limited application due to instability. In this research, nanoliposome containing Ducrosia essential oil has been studied for abundance and size distribution to enhance the essential oil stability for use in drug delivery systems. Methods: Essential oil was prepared and GC-MS were used to identify the extracted compounds. Thin-film hydration method was then used to create small liposome loaded with the extract. Nano-liposomes were made by sonication and filtration methods with molar ratios 7:3, 3:2 and ratios of 3:1 V/V Essential oil to lipid phase. Results: The results showed that size of nanoparticles made by sonication method with molar ratios of 3:2 was 39.49 to 354.4 nm with 80.7 to 19.3 % abundance and PDI of 0.856 Encapsulation Efficiency 38% and  size of liposomes prepared using filtration method was 269.8 nm with 100% abundance and PDI of 0.507 with Encapsulation Efficiency 34% and results of nanoliposomes made by sonication with molar ratios of 7:3 and  ratios of 3:1 was 224.4 to 5330 nm with 93 to 7 % abundance and PDI of 0.229 with Encapsulation Efficiency 46%. The size of liposomes prepared using filtration was 358.9 nm with 100% abundance and PDI of 0.286 with Encapsulation Efficiency 43.5%. Conclusion: It is important to note that the nanoliposomes produced by the sonication are smaller than filtration for use in the liposomal nanoscale system and Encapsulation Efficiency nanoliposomes made by molar ratios 7:3 was more than 3:2 molar ratios.

Fatemeh Direkvand-Moghadam, Mohammad Hossein Mirjalili, Mohammad Naghi Padasht Dehkai, Hassan Rezadoost,
year 22, Issue 85 (3-2023)
Abstract

Background: Tea (Camellia sinensis), belongs to the family Theaceae, is a well-known perennial and evergreen plant that is processed and widely used as a daily drink across the world. Objective: The present study was aimed to explore aromatic composition, catechins content, and metal elements (MEs) profiling of forty-three black tea samples collected across Iran. Method: Black tea samples were prepared from the Iranian Tea Research Institute. MEs content of the plant dried samples was measured by atomic absorption spectrometer. Aromatic composition and catechins content of the studied samples were analyzed by GC-FID, GC-MS and HPLC, respectively. Result: Hexanal (0.3-27.6 %), cis-linalool oxide (0.1-44.7 %), trans-linalool oxide (0.2-48.3 %), linalool (0.2-39.2 %), benzyl alcohol (0.2-38.9 %), phenylethanol (0.1-37.9 %), and (-)-Myrtenol (4.4-26.8 %) were the major volatile oil compounds of the studied samples. The highest content of hexanal, cis-linalool oxide, trans-linalool oxide, linalool, benzyl alcohol, phenylethanol and (-)-Myrtenol was measured in IR5 (Zarin), IR13 (Leil 1), IR23 (Shariat), IR31 (Roozmehr 1), IR34 (Noshiran), IR36 (Roozmehr 3) and IR18 (Amard 2), respectively. Catechin content was ranged from 0.589 ± 0.0285 in IR32 (Roozmehr 2) to 0.65 ± 0.0088 in IR29 (Aramgol 1). The level of the copper, Iron and lead were generally within the safe limitation mentioned in the world. Conclusion: Sample of Roozmehr was characterized with high aromatic compounds, catechins content, and low level of MEs. This information can be interestingly considered by food industrials to process black tea products.


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