Showing 15 results for Fathi
A Pourmotabbed , B Rostamian , G Manouchehri , M Fathi, M Kamal-Nejad , H Sahraei ,
year 3, Issue 12 (11-2004)
Abstract
The prevalance of opioid addiction is releativly high in Iran. Since the mechanism (s) of opioid addiction are not clear, this social problem still remains unresolved. In the present study, the effects of water-alcohol extract of Papaver rhoeas on the acquisition and expression of morphine withdrawal in morphine-dependent N-MARI mice (20-25 g) were investigated. The animals became dependent to morphine and the extract of the Papaver rhoeas was administered during and after induction of morphine dependence. Then, withdrawal syndroms were tested with naloxone (4 mg/kg) injection. Results showed that administration of naloxone after four days morphine treatment (12.5, 25 and 50 mg/kg), induced diarrhea and also increased the number of jumping in animals. The increase was significant for a dose of 50 mg/kg of morphine. On the other hand, administration of the plant extract (25, 50 and 100 mg/kg) did not induce any changes in the feaces or number of jumping behaviour in the animals. Injection of the same extract (25, 50 and 100 mg/kg) 30 min before morphine (50 mg/kg) administration, caused an increase in number of jumping but reduced the diarrhea in animals. Injection of the plant extract (25, 50 and 100 mg/kg) on the test day, 30 min before naloxone administration also decreased the number of jumping and diarrhea in morphine-dependent animals. It could be concluded that the extract of Papaver rhoeas can amilorates the withdrawal syndrom in morphine-dependent mice. Therefore, the extract might be useful for treatment of withdrawal signs in opioid addicts.
A Khaki , M Nouri , F Fathiazad , Hr Ahmadi-Ashtiani , H Rastgar , Sh Rezazadeh ,
year 8, Issue 29 (Supplement 5 2009)
Abstract
Background: Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.
Objective: antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.
Methods: Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily (IP). Diabetes was induced by a single intra peritonea injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis.
Results: Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg (IP) Quercetin (p<0.05) in comparison to control and experimental groups.
Conclusion: Since in our study 15 mg/kg (IP) Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.
Aa Khaki , A Khaki , M Nouri , Hr Ahmadi-Ashtiani , H Rastegar , F Fathiazad , M Ghanbari,
year 8, Issue 29 (Supplement 5 2009)
Abstract
Background: Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.
Objective: Antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.
Methods: Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily(IP) . Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg) .Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for TAC,MDA and Ox-LDL levels and Liver tissues of Rat in whole groups were removed then prepared for Apoptosis analysis by Tunel method.
Results: Apoptotic cells significantly decreased in group that has received 15mg/kg (IP) Quercetin (P<0.05) in comparison to experimental groups (P<0.05).
Conclusion: Since in our study 15mg/kg (IP) Quercetin have significantly Preventive effect on liver cells damages by reducing number of Apoptotic cells in liver, so it seems that using it can be effective for treatment in Diabetic Rat.
A Khaki , F Fathiazad , Hr Ahmadi - Ashtiani , H Rastgar, Sh Rezazadeh ,
year 8, Issue 31 (9-2009)
Abstract
Background: Danae racemosa is used medicinally and as a culinary spice. Its constituents are stated to have antithrombotic, antihepatotoxic, antinociceptive and antioxidant. Objective: Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Methods: Wistar male rat (n=30) were allocated into three groups, control (n=10) and two test groups (each of ten). Animals in test groups were subdivided into groups of 2 that received fresh extract equivalent to 200 & 400 mg/Kg/Rat/day Danae racemosa extract. Fresh Danae racemosa extract was administered with gavages for 28 consecutive days. Animals were kept in standard condition. On twenty eighth day, the testes of rats in the all groups were removed and sperm was collected from epididymis and was prepared for analysis. Results: The percentage of sperm viability and motility in both test groups significantly increased (p<0.05), but the sperm concentration significantly increased only in the group that received the high dose of Danae racemosa extracted, (p<0.05). It was evident that there was no difference on sperm morphology and testis weight in test groups comparing to control group. Conclusion: In our study Danae racemosa extract has significantly affected the sperm number and percentage of viability and motility it seems that using 400 mg/Kg/Rat/day of Danae racemosa extract is effective in sperm health parameters.
B Gholamhosini , A Khaki , Hr Ahmadi-Ashtiani , Sh Rezazadeh , H Rastegar , F Fathiazad , M Ghanbari ,
year 8, Issue 32 (12-2009)
Abstract
Background: Onion has significant blood sugar lowering action. Objective: Antioxidants have essential effect on spermatogenesis and sperm parameters. Streptozotocin can dioceses oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Methods: Wistar male rat (n=30) were allocated into three groups, control group (n=10) and oninon (O) group that received 1cc/rat (gavages) (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus15mg/kg (O), daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily(gavages). Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg) .Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testestrone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Results: Sperm population, percentage of sperm viability and motility and Serum total testosterones significantly increased in group that has received 1cc/rat onion (p<0.05) in comparison to control and experimental groups. Testes weights in streptozotocin group significantly decreased in comparison to control group (p<0.05). Conclusion: Since in our study 1cc/rat onion have significantly Preventive effect on Sperm percentage of viability and motility and serum total testosterones by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.
A Khaki , F Fathiazad , Hr Ahmadi - Ashtiani , Sh Rezazadeh , H Rastegar , Am Imani ,
year 9, Issue 33 (supplement 6 2010)
Abstract
Background: The major concern in diabetes is increased oxidative stress. Maintaining a balance between reactive oxygen species (ROS) and antioxidants is a major mechanism in preventing damage from oxidative stress. Quercetin (QR) is a well-known flavonoid and a strong antioxidant derived from the onion, Allium cepa, and it has been shown to reduce oxidative stress in the long-term treatment of streptozotocin (STZ)-induced diabetes in animals.
Objective: We aimed to study on beneficial effects of QR & Allium cepa on regulation of blood glucose in diabetic model.
Methods: Seventy male 8-week-old Wistar albino rats weighing 250 ± 10 g were obtained, the rats were divided into seven groups comprising ten animals in each group. Diabetes induced with single intraperitoneal injection of STZ. Diabetic rats treated with quercetin (15mg/kg/day, started 48 hours after STZ injection) and Allium cepa juice (2cc/rat/day, started 48 hours after STZ injection). After 28 days treatment, analysis on blood glucose and insulin was done.
Results: The mean TAC showed a significant increase (p < 0.05) in the QR compared to the control, STZ and STZ + QR groups. Both QR and onion Juice had significant effect in lowering blood glucose (355.3 ± 104 mg/dl and 353.4 ± 103 mg/dl respectively) (p<0.05) in STZ induced diabetic animals. Both QR and onion juice had lowering effect in Serum insulin in healthy animals and significant increasing effect on serum insulin level in diabetic animals.
Conclusions: Both QR and onion juice had good effect in modifying diabetes related biochemical parameters and they are recommended to be studied more in order to being used in the therapy.
M Jamshidi , Hr Ahmadi-Ashtiani , Sh Rezazadeh , F Fathiazad , M Mazandarani , A Khaki ,
year 9, Issue 34 (5-2010)
Abstract
Background: Plants are rich in phenolics (phenolic acids, flavonoids and Tannins). The interest in phenolics has been increased by recent reports of their antioxidant activities. These antioxidant nutrients from food sources reduce the harmful effects of oxidative stress and offer health advantages. Objective: Total phenolics, flavonoids contents and antioxidant capacity of seven culinary plants of Mazandaran province were investigated and compared to Rosemary. Methods: Methanolic extracts of dried powdered aerial parts of plants were prepared. Total phenolic compounds in extracts were determined spectrophotometrically using the Folin–Ciocalteu reagent. Antioxidant activity assessed by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: The total phenolic content of plants extracts ranged from 38.27 to 58.45 mgGAEg-1 (Gallic acid equivalent/g dried weight), flavonoid content ranged from 25.5 to 182.23 mgQUEg-1 (mg Quercetin equivalent/g dried weight) and IC50 ranges from 55.52 to 489.9 μgml-1. Among the 7 different plants, Marrubium vulgare contained a higher level of phenolics and high antioxidant capacity compared to Rosemary, while Mentha spicata had the lowest content of phenolics. Conclusion: The antioxidant capacity determined by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method was lowest for of Mentha spicata. These results clearly showed the antioxidant activity could be correlated with the phenolic components content in the extracts.
Aa Khaki , F Fathiazad , Hr Ahmadi - Ashtiani , H Rastegar , Am Imani , A Khaki , R Hajiaghaii ,
year 9, Issue 36 (12-2010)
Abstract
Background: In the industrial world, almost everyone is unavoidably exposed to ambient electromagnetic field (EMF) generated from various technical and household appliances. Objective: Controversy exists about the effects of EMF on various tissues of the living bodies. Uterus play an important role in reproductive and the effects of EMF on its tissue is worthy of investigation. Methods: In order to examine this, 40 rat were selected and kept for one weeks in quarantine and 20 rats subdivided to two groups were exposed to 50 Hz (non-ionizing radiation) for 6 weeks, one of this exposed group received 0.7 g/kg Ocimum basilicum, other experimental group just received 0.7 g/kg Ocimum basilicum, The materials were processed and observed apoptosis under a light microscope. Results: In the experimental rats apoptotic cells showed significantly increased in emf groups, but in exposed group that received Ocimum basilicum ,apoptotic cells were decreased (p<0.05). Conclusion: As exposure to EMF may result in pathological changes then lead to sub fertility and infertility, Since in our study 0.7 g/kg Ocimum basilicum have significantly Preventive effect on uterus cells damages by reducing number of Apoptotic cells, so it seems that using it can be effective for treatment in emf exposed Rat.
A Fathi, Ma Sahari , M Zangiabadi , M Barzegar ,
year 10, Issue 39 (9-2011)
Abstract
Background: Autoxidation is considered to be the main route of edible oil deterioration, which produces undesirable odors and flavors during storage and heating. The unpleasant flavor of oxidized oil has been attributed to primary and secondary products of unsaturated fatty acids. The microwave heating is a rapid method when compared with other methods of heating. Objective: In this research, the effect of microwave heating on the oxidative stability of soybean oil amended with either of Satureja hortensis L. (SHEO) and Zataria multiflora Boiss (ZMEO) essential oils was evaluated and compared with synthetic antioxidant (butylated hydroxyl toluene= BHT 200 ppm). Methods: Soybean oil containing with SHEO (200 and 1000 ppm), ZMEO (200 and 1000 ppm) and BHT (200 ppm) were heated using microwave oven (600 W) for 25 min and spectrophotometer was used to determine UV absorption. Peroxide value (PV), conjugated dienes (CD) and conjugated trienes (CT) indices were measured. Results: Statistical results showed that PV after 25 min were increased in all treatments and the stability of soybean oil treated with synthetic antioxidant (BHT, 200 ppm) was higher than oil treated with the studied essential oils (SHEO and ZMEO, 200 and 1000 ppm). Diene and triene indices increased during the heating. There was a positive correlation between PV, diene and triene indices during the process of microwave heating. Conclusion: The two essential oils (SHEO and ZMEO, in concentrations of 200 and 1000 ppm) showed fairly good antioxidant activities as compared with BHT (200 ppm) in soybean oil control sample (refined- bleached- deodorized soybean oil without any additives).
A Fathi , Ma Sahari , M Barzegar , H Naghdi Badi ,
year 12, Issue 45 (2-2013)
Abstract
Background: It has been considered by researchers to study the possibility of replacing chemical food additives (synthetic antioxidants) by natural products (medicinal plants). Objective: This study investigated the antioxidant properties of Satureja hortensis L. essential oil (SHEO) on safflower oil oxidation. Methods: Different assays have been used to evaluate the antioxidant activity of SHEO: total phenol content (TPC), DPPH•, ABTS+•, ferric thiocyanate (FTC), β - carotene bleaching. For evaluation of SHEO effect on safflower oil oxidation, peroxide value (PV), conjugated dienes (CD), and tiobarbituric acid (TBA) indices were compared with BHT (a synthetic antioxidant). Results: TPC of SHEO was determined to be 293.7 mg gallic acid equivalent in 1 ml of sample and IC50 was 0.71 mg/ml in DPPH•. 0.4 and 0.1 mg/ml of SHEO at all time (1, 5, 10, 15 min) showed the highest and lowest antiradical ABTS°+ activity (118.2 and 26.6 μg/ml AscAE =Ascorbic acid equivalent) in 15 min. In FTC and FTC-TBA, 8 mg/ml SHEO showed the highest activity. In β-carotene bleaching of 0.1-2 mg/ml SHEO, 0.1 has the minimum (%9.02), and 1 and 2 has the maximum inhibitory effects (%34.33 and %36.86 inhibitions). 4 mg/ml of SHEO had the highest inhibitory effect in the safflower oil test and that peroxide does not have significant difference with 0.1 mg/ml BHT. Conclusion: Antioxidant activities of SHEO concentrations increased in all indices (p< 0.05) and various concentrations were able to slow down the oxidation process.
F Fathi , Sh Oryan , M Rafieian , A Eidi ,
year 14, Issue 54 (5-2015)
Abstract
Background: Recent studies have suggested that Mentha longifolia L. extracts (ME) have antioxidant activities. Objective: In this paper, attempts were made to determine the effect of MEin rat stroke model. Methods: Five groups (n=14) were studied the first and second groups (control and sham) received intraperitoneal injection with daily distilled water. The other three groups received 50, 100 and 200 mg/kg/day of the ME for 21 days. Two hours after the last dose, each main group was subdivided into middle cerebral artery occlusion (MCAO) operated for infarct volume assessment and intact subgroup for the assessment of brain and serum antioxidant activity and lipid proxidation of brain and serum, respectively. Results: Pretreatment with ME resulted in a significant reduction in total infarct volume. ME significantly increased antioxidant activity in penumbra and core in comparation with the control. The antioxidant activity of serum in the 100 mg/kg/day group was significantly higher than that of the control group. Malondialdeyde (MDA) level in the penumbra area was significantly elevated in the control group in comparison with other pretreated groups. ME at dose of 100 mg/kg significantly decreased MDA level in core area scale to the control. Moreover, ME with the doses of 100 and 200 mg/kg/day reduced MDA level of serum. Conclusion: ME may protect ischemia/reperfusion induced brain injury by increase in antioxidant activity, decrease in lipid proxidation and reduction in infarction volume.
Z Rabiei , F Fathi , S Asgharzade , M Rafieian-Kopaei ,
year 16, Issue 62 (5-2017)
Abstract
Background: Lavender belongs to the Labiatae family and possesses antioxidant acivity.
Objective: The present study was conducted to evaluate the protective effect of lavender extract on infarct volume and its possible mechanisms in an experimental model of stroke.
Methods: Lavender extract (100, 200 mg/kg body weight, ip) was injected for 20 consecutive days. Two hours after the last dose, cerebral artery ligation surgery was performed and 24 hours after induction of ischemia, infarct volume was assessed. Also the amount of serum nitric oxide (NO) level was measured.
Results: Treatment of rats with lavender extract at a dose of 200 mg for 20 days resulted in a significant decrease in the infarct volume caused by stroke in penumbra area (cortex) and the core (sub-cortical) of brain compared to the control (P=0.044, P=0.047, consecutively). Lavender extract at a dose of 200 mg significantly increased blood levels of nitric oxide.
Conclusion: The results of this study suggest that Lavender extract protects brain against ischemia and reduces infarct volume in rats subjected to ischemia. The mechanism may be related to augmentation in endogenous antioxidant defense in the rat brain. Lavender plant extracts with increasing levels of endothelial nitric oxide, by inhibiting the decrease in cerebral blood flow reduced infarct volume.
S Sattarzad Fathi, H Hassanian-Moghaddam, Sh Shadnia, N Zamani, M Rahimi,
year 18, Issue 70 (5-2019)
Abstract
Background: Because in today's societies, not only the nutritional role of plants and fungi is very high, but also the main basis of many drugs are plants and plant products, therefore one of the problems of clinical toxicologists is the toxicity of these products.
Objective: This study aimed to define the prevalence, symptoms, complications, effective treatments, type of substance use, the cause of intake, location, gender distribution and age of poisoning with herbs and mushrooms in hospitalized patients of Loghman Hakim Hospital in Tehran.
Methods: This is a retrospective study with questionnaire formulation. Patients reviewed from the beginning of 2007 to the end of 2013.
Results: of 103 cases of poisoning with plants and mushrooms 64.1% were male, and 35.9% were female. The mean age of patients was 21.59 ± 30.51 years. The most commonly used substance is Datura (34%). On the other hand, sole mushroom ingestion was found in 5.8% of cases. The most significant source of plants and mushrooms was reported from herbal-shops (27.18%). The reason behind intake was either random or euphoria. Four patients were admitted to the intensive care unit, and three patients needed intubation. The mean hospital stay was 1.64 ± 1.28 days. Ninety percent of the treatments were supportive, and 75.7% of the patients recovered without any clinical complications.
Conclusion: Poisoning with toxic plants and mushrooms is one of the frequent causes of intoxication that is often accidental. In children poisoning is inadvertent, but youth are more likely wanted to have euphoria. Treatment strategies are supportive and symptomatic.
Nooshin Fathi, Farzaneh Tafvizi, Amir Mirzaie,
year 19, Issue 74 (6-2020)
Abstract
Background: The use of medicinal herbs in traditional medicine has been important and many of these plants have antioxidant, anti-inflammatory, antimicrobial and anti-cancer effects. Objective: The aim of this study was to evaluate the antibacterial and anti-cancer effects of Artemisia turcomanica extract on gastric cancer cell line (AGS) and its effect on expression of cyclin D1 and cyclin E genes. Methods: At first the alcoholic extract of A. turcomanica was prepared and its antibacterial effect was evaluated using MIC (Minimum Inhibitory Concentration) method. Subsequently, the cytotoxic effect of extract on AGS cell line was studied using MTT method after 24, 48 and 72 hours. Moreover, the apoptotic effect of extract was determined via annexin FITC-V and PI staining. Finally, the gene expression of cyclin D1 and cyclin E were measured using Real Time PCR method. Results: The antibacterial results show that the A. turcomanica extract had significant effect on Escherichia coli. In addition, the results of MTT assay indicated that the maximum cytotoxic effect of A. turcomanica extract was observed in 24 h and over time, its cytotoxic effects were decreased. The maximum apoptotic effect and minimum necrosis effects was observed in 48 h. Also, the Real Time PCR results reveal that the gene expression of cyclin D1 and cyclin E1 was down-regulated. Conclusion: The results of this study show that the
A. turcomanica extract had cytotoxic and apoptotic effects on AGS cell line and it can be used as anticancer candidate in cancer therapy and prevention after further studies.
Asma Soleimani, Mohammad Fathi, Mustafa Bahrami,
year 23, Issue 89 (3-2024)
Abstract
Background: Some activities, such as acute activity, can cause an increase in inflammatory indicators and muscle damage in the blood. Objective: The aim of the effect of taking ginger supplement along with acute extroverted and introverted activity was on some inflammatory indicators and muscle damage in the serum of obese girls. Methods: After measuring the maximum oxygen consumption, 50 girls with a body mass index of over 30 kg/m2, age 24.89 ± 73.7 years, height 159.02 ± 5.20 cm and weight 87.77 ± 88.8 kg were randomly divided into 5 groups including: experimental group 1 [supplement+ extrovert (n = 10), experimental group 2 [supplement + introvert] (n = 10), placebo + extrovert group (n = 10), placebo + introvert group (n = 10)b , and control group (n = 10) became The training protocol included acute extroverted and introverted activity [positive and negative incline on the treadmill] was implemented. Supplement groups consumed 2 grams of ginger powder daily for 4 weeks. After fasting for at least 8 hours, blood samples were collected before and immediately after any type of activity to measure serum hs-CRP and ck levels. To examine intra-group changes, t-dependent and for inter-group comparisons, analysis of covariance with inter-group factor was used (P = 0.05). Results: The t-dependent results of this research showed that acute extroverted and introverted exercise in the pre-test and post-test, respectively, caused a significant increase in the levels of hs-CRP and CK in the supplement and placebo groups (P ≤ 0.05). Also, the results of covariance analysis showed that taking ginger supplement for four weeks after acute extroverted and introverted activity had a significant effect on hs-CRP levels (P = 0.04) between supplement and placebo groups. CK levels (P = 0.09) had no significant effect between the supplement and placebo groups. Conclusion: Increased levels of inflammation and muscle damage in introverted acute exercise are greater than in extroverted. According to the results of this research, daily consumption of ginger supplements can reduce inflammation and muscle damage caused by physical activity.
