Cleome belongs to Capparidaceae family which are annual or perenial forbs and or shrubs, often with bottomnal coverage. Research in other parts of world has determined that some species of this plant such as C. drosiflolia, C. hirta, C. gynandropsis, C. chrysantha and C. gunandra and several other species have had antibacterial effects and or native people use some of these species as traditianal drugs. Considering that different species of Cleome have wild separation in Iran and little research have been done about them. Aerial parts of Cleome coluteoides was collected from North East of Tehran (Khojir National Park), in June 2002 and was identified by Dr.Mozafarian. A voucher specimen has been deposited at the Herbarium of Research Institute of Forests and Ranglans (TARI), Tehran, Iran. Air-dried aerial parts at the plants were ground and subjected to hydrodistillation for 2h using a Clevenger-type apparatuse to give a yellew oil in (0.6% w/w) yield. Also some of the C. coluteoides was extracted with nonpolar solvent (n-hexan) and then the solvent was evaporated. The essential oil obtained from two methods were ananlysed through combination of GC (retention-indices) and GC/MS. The major components in essential oils of C. coluteoides In hydrodistillation method: Carvacrol (46.124%), Phethalic acid (10.682%) Heneicosane (5.382%) Solvent extraction method: Xylene (29.923%), Dodecane (14.435%) Essential oil obtained from extraction with water, has shown 46mm inhibitory effect on Bacilus cerius and 18mm effect on Staphilocucus ureus.

Background: Due to increasing drug resistance among pathogenic bacteria toward current and new generation antibiotics, researchers have been investigating to find herbal antimicrobial agents as alternative medicines. Objectives: In this study, the inhibitory effects of alcoholic extract of Zataria multiflora Boiss leaves, flowers and root on methicillin-resistant Staphylococcus aureus was evaluated and compared. Methods: 50 grams of dried thyme leaves, flowers and root were added to 250 ml hydroethanol separately, and were then distilled. The antibacterial properties of the extracts were assessed by Broth Dilution Method and after determining dried weights of the extracts per ml, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of them were obtained. Finally, by using Thin Layer Chromatography (TLC), effective compounds of the extracts were detected. Results: Thyme leaves alcoholic extract at 3/031 mg/ml and 6/062 mg/ml concentration, flowers alcoholic extract at 5/687 mg/ml and 11/375 mg/ml concentration and root alcoholic extract at 5/437 mg/ml and 10/875 mg/ml concentration, in order, were able to inhibit and kill all the strains and no difference were observed between them. Analysis of the chromatograms showed that thymol and carvacrol are the main compounds of this herb. Conclusion: However clinical application of herbal extracts and oils sounds valuable because of their less side effects in comparison with current drugs, in order to clinically use thyme alcoholic extracts, further investigations must be done in clarifying the mechanism of action of its effective compounds on microbial agents.