Showing 13 results for Ahmadi-Ashtiani
Ah Jamshidi , Hr Ahmadi-Ashtiani , B Gholamhouseyni , S Bokaei ,
year 6, Issue 24 (12-2007)
Abstract
Background: In poultry industry the most common and important mycotoxin is aflatoxin, and decreasing its economical and sanitary damages by using effective and inexpensive substances is the target of most of the researchers. Objective: Since the pathologic effects of aflatoxin toxicity are more important in liver, kidney and muscles, and due to the mechanism of formation of the damages and also taking in to account the other studies on silymarin effects (extract of Silybum marianum) in preventing various liver and kidney diseases, the silymarin effects reviewed on preventing the losses caused by aflatoxin due to the poultry breeding farms conditions in Iran. Methods: In this study 56 one-day chicken, divided in to 4 groups, each group contains 14 chickens. First group were feed with normal allotment, second group with normal allotment with added 1 mg/Kg aflatoxin, third group with normal allotment with 800 mg silymarin per kg body weight and with added 1 mg/Kg aflatoxin, and after 42 days biochemical, autopsy and histopathology surveys have been done on the samples. Results and Conclusion: According to the reviewed data and comparing the results of studied groups, silymarin can be recommended for decreasing the damages caused by aflatoxin during the period of breeding broiler chickens.
H Rastegar , Hr Ahmadi-Ashtiani , P Omidian , S Bokaei , Sh Rezazadeh ,
year 7, Issue 27 (8-2008)
Abstract
Background: Cassia angustifolia Extract (CA) has indication as a colon irritant. For rapid and full action of this extract or their derivatives need to high dosage of them. CA can have toxic effects on different organ of body, therefore it is necessary to study effects of them on liver and kidney. Objective: In this study effect of Cassia angustifolia syrup on liver and kidney of rat were assigned by analysis of biochemical factors which related to these organs. Method: 40 rats randomly divided in two groups with 20 members. First group was received 0.9 ml/kg CA containg of 1.85 mg CA extract in 30ml sterile water. Second group was received only 30ml sterile water. After 1, 24, 48 and 72 hours blood samples were taken from these groups and then analysis of biochemical factors related to liver and kidney activity were done. Also, the clinical conditions were recorded four times a day. Results: diarrhea as a clinical condition showed only in the first group during 72 hours. 1 hour after receiving CA or water in both groups the ALP, ALT and AST showed no changes but after 24, 48 and 72 hours these factors showed significant differences between two groups. There were no significant differences between bilirubin levels after 24 hours in both groups but there were after 48 and 72 hours. There were no significant differences in levels of nitrogen urea during 72 hours. Conclusion: Considering to significant changes showed by statistical analysis of biochemical factors which results in two groups after study and also due to no significant changes in clinical condition, We suggest more study about effects of CA is necessary.
A Khaki , M Nouri , F Fathiazad , Hr Ahmadi-Ashtiani , H Rastgar , Sh Rezazadeh ,
year 8, Issue 29 (Supplement 5 2009)
Abstract
Background: Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.
Objective: antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.
Methods: Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily (IP). Diabetes was induced by a single intra peritonea injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis.
Results: Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg (IP) Quercetin (p<0.05) in comparison to control and experimental groups.
Conclusion: Since in our study 15 mg/kg (IP) Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.
Aa Khaki , A Khaki , M Nouri , Hr Ahmadi-Ashtiani , H Rastegar , F Fathiazad , M Ghanbari,
year 8, Issue 29 (Supplement 5 2009)
Abstract
Background: Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.
Objective: Antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.
Methods: Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily(IP) . Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg) .Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for TAC,MDA and Ox-LDL levels and Liver tissues of Rat in whole groups were removed then prepared for Apoptosis analysis by Tunel method.
Results: Apoptotic cells significantly decreased in group that has received 15mg/kg (IP) Quercetin (P<0.05) in comparison to experimental groups (P<0.05).
Conclusion: Since in our study 15mg/kg (IP) Quercetin have significantly Preventive effect on liver cells damages by reducing number of Apoptotic cells in liver, so it seems that using it can be effective for treatment in Diabetic Rat.
M Nassiri , A Khaki , Hr Ahmadi-Ashtiani , Sh Rezazadeh , H Rastgar , Sh Gharachurlu ,
year 8, Issue 31 (9-2009)
Abstract
Background: Ginger rhizome (Zingiber officinale R., family: Zingiberaceae) is used medicinally and as a culinary spice. The medicinal use of ginger dates back to ancient China and India. Objective: Ginger and its constituents are stated to have antiemetic, antithrombotic, antihepatotoxic, anti-inflammatory, stimulant, cholagogue and antioxidant.Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Methods: Wistar male rat (n=40) were allocated into three groups, control group (n=10) and ginger (G) group that received 100mg/kg-perday (oral), (n=10), and Diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus ginger 100mg/kg-perday (G), daily for, 4 weeks, respectively however, the control group just received an equal volume of distilled water daily(IP). Diabetes was induced by a single (IP) injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for TAC, MDA and sperm parameters and testis tissues of Rat in whole groups were removed then prepared for analyzes. Results: TAC, MDA and sperm parameters significantly decreased in diabetic group.treatment groub that has received Ginger 100 mg/kg (oral) show increasing in TAC and sperm parameters (p<0.05) in comparison to experimental groups (p<0.05). Conclusion: Since in our study 100 mg/kg (oral) ginger have significantly Preventive effect on sperm parameters, so it seems that using it can be effective for treatment in Diabetic Rat.
B Gholamhosini , A Khaki , Hr Ahmadi-Ashtiani , Sh Rezazadeh , H Rastegar , F Fathiazad , M Ghanbari ,
year 8, Issue 32 (12-2009)
Abstract
Background: Onion has significant blood sugar lowering action. Objective: Antioxidants have essential effect on spermatogenesis and sperm parameters. Streptozotocin can dioceses oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Methods: Wistar male rat (n=30) were allocated into three groups, control group (n=10) and oninon (O) group that received 1cc/rat (gavages) (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus15mg/kg (O), daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily(gavages). Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg) .Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testestrone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Results: Sperm population, percentage of sperm viability and motility and Serum total testosterones significantly increased in group that has received 1cc/rat onion (p<0.05) in comparison to control and experimental groups. Testes weights in streptozotocin group significantly decreased in comparison to control group (p<0.05). Conclusion: Since in our study 1cc/rat onion have significantly Preventive effect on Sperm percentage of viability and motility and serum total testosterones by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.
Aa Khaki , A Khaki , Hr Ahmadi-Ashtiani , H Rastegar , Sh Rezazadeh , D Babazadeh , A Zahedi , Z Ghanbari ,
year 9, Issue 33 (supplement 6 2010)
Abstract
Background: Antioxidants have essential effect on tissue regeneration after cells injury. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Ginger rhizome and carrot seed are strong antioxidants and long-term treatment of Streptozotocin induced–diabetic animals with these herbs, has been shown to reduce oxidative stress.
Objective: Evaluation to treatment effect of Ginger rhizome and extract of carrot seed on nephropathy after diabetes inducement.
Methods: Wistar male rat (n=70) were allocated into seven groups, control group, carrot seed extract group, ginger group, control- Diabetic group received 55mg/kg (IP) streptozotocin (STZ), treatment diabetic group that received carrot seed extract, treatment diabetic group that received ginger and treatment diabetic group that received carrot seed extract plus ginger. Animals were kept in standard condition. In 30 day after inducing diabetes, 5ml blood were collected for analyzing of TAC and MDA levels, and kidney tissues of Rats were removed in all groups then prepared for analysis.
Results: Pathological changes in diabetic group which received carrot seed and ginger together was decreased compared to control group. The rate of serum TAC significantly increased in diabetic groups which received carrot seed and ginger together significantly in comparison to control-diabetic group (p<0.05).
Conclusion: Since in our study 25 mg/kg carrot seed extract and 100 mg/kg ginger have prevented kidney tissue injury by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for treatment nephropathy in Diabetic rats.
Hr Ahmadi-Ashtiani , Sh Rezazadeh , E Barkhordari , N Hekmat-Nazemi , M Baghaei , S Bokaie , H Rastegar ,
year 9, Issue 33 (supplement 6 2010)
Abstract
Background: As increased outdoor activities of people, they are more exposed to UV radiation, which causes harmful effects on skin. Silymarin has protective potentials against DNA damage and non-melanoma skin cancers, and also it has anti-oxidant, anti-inflammatory, and immune regulation characteristics. Nano formulation of plant material showed to be more effective than common ones.
Objective: In present study, nano-formulated silymarin was used in a cream, and its effects were investigated against destructive effects of UV rays.
Methods: Silymarin standardized extract was prepared, nano formulated in cream base, and applied to skin of the animals. Four groups of animals (n=15) were examined Group 1 without any treatment (control), Group 2 Vaseline, Group 3 nano-formulated silymarin extract cream, and Group 4 silymarin-carnosine. UV was radiated on the shaved area of the four groups for 45 minutes with dose of 180 mJ/cm2. Dermal scaling, skin irregularity, erythema, skin hyperpigmentation, and edema were evaluated in animals. Histopathological observation was done on the skin samples of the animals.
Results: silymarin nano-formulation was effective in decreasing both physical damages and histopathological injuries significantly.
Conclusion: from the observed results, it can be concluded that the cream containing nano-formulated silymarin can prevent UV radiation lesions better than pure silymarin.
M Jamshidi , Hr Ahmadi-Ashtiani , Sh Rezazadeh , F Fathiazad , M Mazandarani , A Khaki ,
year 9, Issue 34 (5-2010)
Abstract
Background: Plants are rich in phenolics (phenolic acids, flavonoids and Tannins). The interest in phenolics has been increased by recent reports of their antioxidant activities. These antioxidant nutrients from food sources reduce the harmful effects of oxidative stress and offer health advantages. Objective: Total phenolics, flavonoids contents and antioxidant capacity of seven culinary plants of Mazandaran province were investigated and compared to Rosemary. Methods: Methanolic extracts of dried powdered aerial parts of plants were prepared. Total phenolic compounds in extracts were determined spectrophotometrically using the Folin–Ciocalteu reagent. Antioxidant activity assessed by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Results: The total phenolic content of plants extracts ranged from 38.27 to 58.45 mgGAEg-1 (Gallic acid equivalent/g dried weight), flavonoid content ranged from 25.5 to 182.23 mgQUEg-1 (mg Quercetin equivalent/g dried weight) and IC50 ranges from 55.52 to 489.9 μgml-1. Among the 7 different plants, Marrubium vulgare contained a higher level of phenolics and high antioxidant capacity compared to Rosemary, while Mentha spicata had the lowest content of phenolics. Conclusion: The antioxidant capacity determined by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method was lowest for of Mentha spicata. These results clearly showed the antioxidant activity could be correlated with the phenolic components content in the extracts.
A Zahedi , A Khaki , Hr Ahmadi-Ashtiani , H Rastegar , Sh Rezazadeh ,
year 9, Issue 35 (9-2010)
Abstract
Background: Ginger rhizome (Zingiber officinale R., family: Zingiberaceae) is used medicinally and as a culinary spice and has anti-oxidant and cell-protective effects in animals and humans body. Objective: The aim of this study was to investigate the influence of Ginger rhizome toxicity of Gentamicin on sperm parameters in male rats. Methods: Forty Wistar male rat (n=40) were allocated into four groups, control (n=10) and test groups (n=30), that subdivided into groups of 3 that received ginger rhizome powder (100 mg/kg/day), gentamicin group that received, 5 mg/kg/day and ginger group that received, 5 mg/kg/day gentamicin additionally, for 30 consequence day. Animals were kept in standard conditions. In thirty day the testes tissue of Rats in whole groups were collected. Results: Ginger administration caused a marked increase in the testosterone concentrations of the rats even in spite of receiving 5 mg/kg/day gentamicin in compared with the control and gentamicin treated groups. Conclusion: Ginger rhizome is able to overcome reproductive toxicity of gentamicin and induces spermatogenesis probably mainly through the elevation of testosterone levels.
Sh Shahreari , A Khaki , Hr Ahmadi-Ashtiani, Sh Rezazadeh , R Hajiaghaei,
year 9, Issue 35 (9-2010)
Abstract
Background: Danae racemosa is a strong antioxidant and antioxidants have significant effects on spermatogenesis, sperm biology and oxidative stress, and changes in antioxidant capacity are considered to be involved in the pathogenesis of chronic diabetes mellitus. Objective: Because STZ causes testicular dysfunction and degeneration under situations of experimentally induced diabetes in animal models, we aimed in this study Danae racemosa’s effects in decreasing the harmful effects of STZ on testicular and sperm functions b. Methods: Male Wistar rats (n = 40) were allocated into four groups: Group 1a: Control rats given 0.5 ml of 20% glycerol in 0.9% normal saline. Group 1b: Control rats given 0.5ml of 0.5 ml citrate buffer (pH4.0).Group 2: streptozotocin (STZ) treated rats. Group 3: rats given danae racemosa 400 mg/kg (gavage). Group 4: STZ treated rats given danae racemosa 400 mg/kg (gavage). Animals were kept in standard conditions. At 28 days after inducing diabetics, 5 mL blood was collected for measuring testosterone. Results: Total serum testosterone increased significantly in the group treated with danae racemosa (p < 0.05) compared with control groups. Testis weights in the diabetic groups decreased significantly in comparison with controls (p < 0.05). Conclusion: Danae racemosa had a significant protective effect on the diabetes-induced deteriorations in serum total testosterone, by reducing the levels of reactive oxygen species in serum. Therefore, it could be effective for maintaining healthy in diabetic rats.
Hr Ahmadi-Ashtiani , Sh Rezazadeh , K Safipourian , K Afraz , A Khaki , H Rastegar,
year 9, Issue 35 (9-2010)
Abstract
Background: Alcoholic mothers who consume ethanol give birth to infants with high percentage of hepatic diseases. Alcohol can cause cellular damage in different tissues, including liver. Finding a drug which is effective and efficient in reducing ethanol misuse consequences during pregnancy can assist the decrease of harmful effects of this habit. Objective: This research aims to study the effects of oral administration of silymarin in preventing consequences of ethanol on liver during pregnancy. Methods: 45 female rats were randomly divided into 3 groups, each including 15 ones. After the first day of pregnancy, the study was performed as follows. The first group was given distilled water. The second group received ethanol equivalent to 35% of the total required calorie. Furthermore, the third group received the same amount of ethanol plus 200 mg/kg silymarin. At day 21, biopsy of liver tissue of the born infants was carried out for light microscopy studies. Results: Findings of the present study indicate that, in the group which received ethanol, this extract can have a considerable effect on decreasing the hepatic cellular death as well as reducing hepatic hyperemia. Conclusion: Oral consumption of silymarin extract can reduce the damage and inflammation in liver tissue of infants born from alcoholic mothers.
Nilofar Eradati, Fatemeh Tajabadi, Hamid Reza Ahmadi-Ashtiani, Shamsali Rezazadeh, Mahdi Taherian, Hossein Rastegar,
year 20, Issue 77 (1-2021)
Abstract
Background: Hydroquinone, arbutin and kojic acid are herbal-based skin whitening compounds that are used in many cosmetics. Today, the lack of side effects of arbutin and kojic acid has made them a valuable alternative to hydroquinone. Due to the toxic effects of whitening compounds, it is necessary to control the amount of use of these in the whitening products so that their concentration is within the permissible range. Objective: Therefore, the purpose of this study was to identify and quantify these three compounds in anti-lacquer products. In this research, the best separation of analytes has been tried by changing the HPLC mobile phase. Methods: Different samples were taken from the supply level. After extraction and preparation of samples, the solution was injected into HPLC-UV. In order to optimize the method, three different mobile phases were used to identify the compounds. By comparing the obtained peaks with the standard peaks, the Whitening compounds were identified and quantified. Results: The results showed that hydroquinone and kojic acid were applied in three products and arbutin in one product and the concentration of all the compounds used were within the standard range. Four out of the eight companies surveyed did not correctly identify the compounds used in their products. Conclusion: Reverse Phase HPLC-UV is also one of the best methods for determination and quantification the whitening compounds and water composition: acetonitrile with a ratio of 80:20 v/v is the most appropriate mobile phase in this method.
