Shima Sheikhi, Ali Khamesipour, Tayebeh Radjabian, Zahra Mojallal Tabatabaei, Tooba Ghazanfari,
year 19, Issue 74 (6-2020)
Abstract
Background: Cutaneous leishmaniasis by different species of Leishmania. Pentavalen antimonials as a first line drug for treatment of cutaneous leishmaniasis have several limitations and side effects. Natural products are more considered due to their less toxicity and cost, more efficient, safety and readily available antileishmania agents. Objective: The aim of this study was to explore the effect of hydroalcoholic extract of Glycyrrhiza glabra (licorice) roots and its main component glycyrrhizic acid on promastigote and amastigote of L. major. Methods: Different dosages of hydroalcoholic extract of licorice root, glycyrrhizic acid and Glucantime with promastigote of L. major were incubated at 26 °C for 24 and 48 hours then the percentages of alive promastigotes were measured by MTT assay. The antiamastigote effects of these drugs was examined by microscopic counting of the number of amastigotes in macrophages 24 hours after treating the parasite infected macrophages with them. Promastigote and infected macrophages without any treatment was used as negative controls. Results: The IC50 values of hydroalcoholic extracts of licorice root, glycyrrhizic acid and Glucantime on promastigote of L. major was 1250 ± 0.018, 3000 ± 0.017 and 50 ± 0.043 μg/ml after 24 hours, and 1000 ± 0.016, 3000 ± 0.017 and 25 ± 0.009 μg/ml after 48 hours, respectively. The IC50 values of the licorice root extract, glycyrrhizic acid and Glucantime on amostigate was 500, 1000 and 25 μg/ml, respectively. Conclusion: Hydroalcoholic extract of licorice root and glycyrrhizic acid had cytotoxic effects on promastigotes and amastigotes of L. major.
Tahereh Zadeh Mehrizi, Mostafa Pirali Hamedani, Hasan Ebrahimi Shahmabadi, Mehdi Mirzaei, Mehdi Shafiee Ardestani, Mostafa Haji Molla Hoseini, Nariman Mosaffa, Ali Khamesipour, Ahmad Javanmard, Shamsali Rezazadeh, Amitis Ramezani,
year 19, Issue 74 (6-2020)
Abstract
Background: Treatment of leishmaniasis is a challenge due to problems such as high price and dose of the drug, drug resistance and side effects. Objective: The study aims to introduce plants compounds, which their antileishmanial effects were approved in vivo conditions. Methods: This study as a review article was performed by searching the keywords of “medicinal plants with in vivo antileishmanial effects, nanocarrier, clinical trials, and mechanism of action” in the well-known databases to 2018. In this study, 14 medicinal plant compounds with antileishmanial effects were reviewed and mechanism of action and their in vivo therapeutic effect were evaluated. Results: It was found that while some of these compounds had low antileishmanial effects, their efficacy against leishmaniasis was significantly increased through loading into nanocarriers. Conclusion: This study indicated that active component of medicinal plants especially along with nano carriers can be of interest for the treatment of Leishmania.