Background & Objective: Information on the fungal contamination of consumer tea is limited and this kind of contamination is important for food and therefore a survey was undertaken to determine the fungal contamination of consumer tea in East Azarbaidjan province. Methods: A total of 100 samples were obtained using standard 20 cm collection sond from whole sales and stores in different part of East Azarbaidjan province and examined according standards methods, Samples were cultured in YCFA medium and incubated at 25ºC for maximum 3 weeks and a screening survey for positive samples was carried out. Results: 73 (73%) out of total 100 samples had fungal contamination more than 104 colony forming units/gram (cfu/g), Aspergillus niger, Penicillium Sp, Fusarium Sp, Asergillus fumigatus, Alternaria Sp, Cladesporium Sp, Mucor Sp, Gliocladium Sp and Trichotecium Sp were the most frequently detected microorganisms throughout the survey respectively. Conclusion: The presence of moulds such as Aspergillus Sp, Penicillium Sp, Fusarium Sp, Alternaria Sp, Cladesporium Sp, Mucor Sp, Gliocladium Sp and Trichotecium Sp in consumer tea can lead to the presence of harmful mycotoxins produced by these moulds and then could result in serious toxicity and illness in humans, As the fungal contamination has a strong influence on the ultimate quality of tea, tea end products and food safety, more attention to the quality and safety of tea are necessary.

Background: Trachyspermum ammi is one of the prominent plant in traditional medicine of the east with several medicinal effects such as improvement of stomach disease, digestive disorder, diarrhea, hemorrhoid, bladder stones, respiratory disease and etc. Medicinal plants is one of the important reason of toxicity particularly in children and the elderly therefore, evaluation of the toxic effects of a plant is important.
Objective: The aim of present study was evaluation of toxicity of ajowan oil in experimental animals.
Methods: Initially, essential oil of the plant fruit was extracted to study the acute toxicity to rats by gavage. The animals were treated with 1000 mg/kg of the essential oil for 23 and 45 days to determine chronic. Hematological and biochemical parameters of rats’ blood samples were collected and spleen, kidney, liver and lung of rats were isolated for histopathologic examination.
Results: According to acute studies result lethal dose, 50% (LD₅₀) of ajowan essential oil was about 2294 mg/kg. Chronic evaluation showed that there is no statistical difference between weight, food and water consumption of test and control groups. Further, tissue analysis showed no serious change in examined tissues in the treated rats.
Conclusion: Finally according to results, no chemical parameters of blood and histological pattern of tissue were affected by T. ammi oil; however the oil could be classified as moderately toxic due to its LD₅₀ value.