Background: More than 650 types of leech have been recognized in the world that 50 types of these nutrition of blood mammals. So far in the different Nations Pharmacopeia  not registered drug  for treatment of complications with leech in the world .Chamomile is one of the most widely used and well-documented medicinal plants in the world which used for treatment of anti -spasmodic ,anti-inflammatory and alleviated pain. 
Objective: This experimental study prepared methanolic extract of Matricaria chamomilla L and selected several chemical drugs such as Febantel, Mebendazole, Rafoxanide, Praziquantel and Ivermectin and studied comparison effects on leech species of limnatis. nilotica.
Methods: In this experimental study, 80 number leech species of limnatis nilotica were collected and transfered in glass container which contains 600 ml of spring water placed a number of leech and were accurately recorded paralysis and death from time adding the drugs for 720 minutes.
Results: The results showed that the Mebendazole, Praziquantel and Rafoxanid could not dead the leech but Febantel  with dose of 100 mg with mean time of 440.44 minutes was killed the leeches. Ivermectine had the best effect on leech and methanolic extract of Matricaria chamomilla L (600b mg/ml) could kill the leaches in the average time of 232.22 min (1+) which recommended use in cases of contamination with leech species limnatis nilotica. Results of this study showed that Ivermectine, Albendasole and methanolic extract of Matricaria chamomilla L recommended use in cases of contamination with leech species limnatis nilotica while lack of effect on leech drugs such as Mebendazole, Praziquantel, Rafoxanid and Febantel because of low effect not recommended.

Background: Dorema aucheri (Apiaceae) grows in west and southwest of Iran. Young leaves of this plant are used orally as pickle or for cooking some local foods, as well as for the treatment of parasitic digestive infections and constipation.
Objective: The aim of present study was evaluation of cytotoxic potential and total phenols determination of D. aucheri young aerial parts extract and fractions and its pickle extract.
Method: The dried powders of D. aucheri young aerial parts and pickles were macerated with 80% methanol and fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Cytotoxic activity of the total extract, fractions and pickle extract on HT-29, MDA-MB-231, A549, Hela cancer and normal fibroblast cell lines were assessed by MTT assay. Total phenol contents of all samples were determined by Folin-Ciocalteu method.
Results: The potent cytotoxic activities were shown by PE and CL fractions on normal fibroblast cell line with IC₅₀ equal to 17.66±2.3 and 48.80±1.24 µg/ml, respectively. Other fractions and extracts didn’t have considerable cytotoxic effects on other cell lines. In addition, two mentioen fractions had the lowest total phenols. ME and EA fractions with highest amounts of phenols showed the lowest cytotoxicity.
Conclusion: It is concluded D. aucheri compounds have potential to exhibit toxic effects on healthy cells. On the other hand, there is negative correlation between cytotoxicity and phenol contents of D. aucheri fractions.

Background: Arum rupicola Boiss. (Araceae Family) is used by the native people of southern areas of Iran as a soup called "kardeh soup". Several flavonoids and phenol compounds have been identified from Arum species. Objective: The aim of this study was to evaluate antioxidant effect and total phenol contents as well as cytotoxic activity of the leaves of A. rupicola. Methods: Antioxidant activity of total methanol extract and fractions including n-hexane, chloroform, ethyl acetate and water residue were evaluated using FRAP and DPPH methods. Total phenol content was measured using Folin-Ciocalteu method. Cytotoxic activity of the extract and fractions were investigated against human breast cancer MCF-7, MDA-MB-231, and T47D cell lines by MTT assay. Further phytochemical isolation was done on the water residue using column chromatography. Results: According to the results, water residue showed the lowest IC₅₀ value (186.7 µg/ml) and the total methanol extract showed the most antioxidant power (163.62 mmol FeSO4/100 g extract) and phenol content (135 µmol Gallic acid/g extract). The hexane fraction also showed the highest cytotoxic effect against MCF-7 breast cancer cell line with IC₅₀ equal to 118.9 μg/ml. Phytochemical analysis of the water residue resulted in isolation and identification of three isoflavonoids named orobol, genistein and genistein 8-c-glucoside. Conclusion: Based on the identification of isoflavonoid compounds in this plant, its ability to be used as a phytoestrogenic supplement can be considered in future studies.