Volume 2, Issue 34 (6-2010)                   J. Med. Plants 2010, 2(34): 90-94 | Back to browse issues page

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Haeri M, Shams-Ardakani M, Rahbani M, Izaddust M, Khanavi M. Quantification of 4-hydroxyisoleucine content in treated and untreated Fenugreek seeds using GC. J. Med. Plants. 2010; 2 (34) :90-94
URL: http://jmp.ir/article-1-288-en.html
1- Department of Clinical Biochemistry, Faculty of Medicine, Qom University of Medical Sciences
2- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences , shams@tums.ac.ir
3- Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences
4- Department of Phamacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences
Abstract:   (4208 Views)
Background: There are some reports regarding antidiabetic activity of 4-hydroxyisoleucine, however its hypoglycemic property must be proven completely before presentating as a new drug. Preparing suitable amount of 4-hydroxyisoleucine is nesessary for supporting in vivo studies. Objective: The purpose of the present study was isolation of 4-hydroxyisoleucine, comparing 4-hydroxyisoleucine content in two samples of Fenugreek seeds collected form two distinct parts of Iran and finally the effect of germination and supplementation by isoleucine on 4-hydroxyisoleucine yield. Methods: 4-hydroxyisoleucine was successfully isolated passing seed amino acid extract through ion exchange and silica gel column and then crystallization on methanol. 4-hydroxyisoleucine content in various samples was assessed by GC. Results: Seeds from Northwestern of Iran had higher amount of 4-hydroxyisoleucine in comparison to Central Iran. Furthermore yields from germinating seeds were 2 folds higher than the dried seeds, and could be increased 6 fold by inclusion of isoleucine supplement in the irrigation water. Conclusion: simple procedures can be engaged for improving yields of 4-hydroxyisoleucine isolated from fenugreek up to six-fold by using of germinated seeds supplemented with isoleucine.
Full-Text [PDF 206 kb]   (1740 Downloads)    
Type of Study: Research |
Received: 2014/10/21 | Accepted: 2014/10/21 | Published: 2014/10/21

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