year 16, Issue 61 And S10 (supplement 10 2017)                   J. Med. Plants 2017, 16(61 And S10 ): 141-155 | Back to browse issues page

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The Production of Hairy Roots from (Datura innoxia L.) by Agrobacterium rhizogenes and Effects of Salicylic Acid and Methyl Jasmonate Biological Elicitors on Atropine and Scopolamine Content. J. Med. Plants. 2017; 16 (61) :141-155
Abstract:   (3737 Views)

Background: Using Agrobacterium rhizogenes due to create hairy roots, is a useful method to increase secondary metabolites many plants.

Objective: Purpose of this research is to transgenic hairy roots culture, in order to produce secondary metabolites in Datura innoxia.

Methods: Explants leaf and cotyledon of Datura innoxia were inoculated for two months with A7, A4 and 15834 strains of Agrobacterium rhizogenes; Furthermore injection and Immersion methods were used in this scrutiny. The presence of T-DNA in transgenic hairy roots were confirmed by PCR. Transgenic hairy roots in liquid medium of 1/2MS were cultured. In order to induct elicitors, methyl jasmonate in tow densities of 50µM and 100µM, and salicylic acid in three densities of 1mM, 0.1mM and 0.01 mM were used randomly three times. Atropine and scopolamine content of transgenic hairy roots were examined by HPLC.

Results: The highest and lowest rate of transgenic hairy roots production was respectively related to the strains of A4 and 15834. Best explants for inoculation, leaf with A4 strain and cotyledon with A7 strain, were reported. With highest production rate of hairy roots, Simple deposit using a syringes method was recognized as the best method of inoculation. The effect of salicylic acid at a density of 0.1 mM increases the content of atropine concentrations. Also the results showed that usage of Methyl jasmonate at higher doses (100 µM) reduces the content of atropine and scopolamine.

Conclusion: A. rhizogenes as an appropriate method to produce hairy roots and elicitors the best treatment for increase alkaloids.



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Type of Study: Research | Subject: Biotechnology
Received: 2016/02/4 | Accepted: 2016/08/7 | Published: 2017/02/13

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