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:: Volume 1, Issue 61 And S10 (supplement 10 2017) ::
JMP 2017, 1(61 And S10 ): 167-173 Back to browse issues page
In vitro Evaluation of Methanol Extract of Agrimonia eupatoria on PBMC and 12 Pathogenic Bacteria
Abstract:   (1422 Views)

Background: Today the use of medicinal plants to improve the immune system function and against pathogenic bacteria has been considered.

Objective: In this study, the effect of methanol extract of the aerial parts of Agrimonia eupatoria on peripheral blood mononuclear cells (PBMC) and 12 pathogenic bacteria were investigated.

Methods: The methanol extract of branches, stems, seeds and leaves of Agrimonia eupatoria were prepared and the effect of different concentrations of 50, 100, 500, 1000, 1500, 2000 and 2500 μg/ml of the extract on proliferation of PBMC was evaluated by MTT assay. Also the effect of concentrations of 1, 2, 4, 5, 7 and 10 mg/ml of the extract was tested on 12 pathogenic bacteria by disc method and on nutrient agar media.

Results: The methanol extract of the branch, stem and seed of Agrimonia eupatoria showed the most stimulatory effects on immune system and induced the proliferation of PBMCs up to 8 times. Methanol extract of Agrimonia eupatoria showed antibacterial effects against gram-positive bacteria and the most antibacterial effect was on Bacillus subtilis at a concentration of 4 mg/ml and Staphylococcus aureus at concentration of 7 mg/ml.

Conclusion: The Agrimonia eupatoria methanol extract showed stimulatory effects on the immune system and also antibacterial properties against certain gram-positive pathogenic bacteria. These finding indicate that Agrimonia eupatoria can be considered to use for immunodeficiency patients and moreover to control some bacterial infections.


Keywords: Agrimonia eupatoria, Antibacterial, Immunostimulator, PBMC
Full-Text [PDF 370 kb]   (630 Downloads)    
Type of Study: Research | Subject: Medical Biotechnology
Received: 2017/02/13 | Accepted: 2017/02/13 | Published: 2017/02/13
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In vitro Evaluation of Methanol Extract of Agrimonia eupatoria on PBMC and 12 Pathogenic Bacteria. JMP. 2017; 1 (61) :167-173
URL: http://jmp.ir/article-1-1618-en.html

Volume 1, Issue 61 And S10 (supplement 10 2017) Back to browse issues page
فصلنامه علمی پژوهشی گیاهان دارویی Journal of Medicinal Plants
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