سال 25، شماره 97 - ( 2-1405 )
سال 25 شماره 97 صفحات 56-45 |
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Sonboli A, Hashemianpoor S, Fallahi S, Esmaeili H. Genetic diversity in six Dracocephalum L. species from Iran based on ISSR markers. J. Med. Plants 2026; 25 (97) :45-56
URL: http://jmp.ir/article-1-3861-fa.html
URL: http://jmp.ir/article-1-3861-fa.html
Genetic diversity in six Dracocephalum L. species from Iran based on ISSR markers. فصلنامه گياهان دارویی. 1405; 25 (97) :45-56
چکیده: (8 مشاهده)
Background: Dracocephalum L., comprising 89 species worldwide, is the second-largest genus in the Nepetinae subtribe of the Mentheae tribe within the Lamiaceae family. In the flora of Iran, the genus is represented by 10 species. Dracocephalum species are known for their medicinal properties, including anticancer, antioxidant, and anti-inflammatory effects, as well as various other therapeutic uses. Objective: This study aimed to evaluate the effectiveness of Inter-Simple Sequence Repeat (ISSR) markers in differentiating Dracocephalum species and to assess the genetic diversity among various species within the genus. Methods: Thirteen populations from six Dracocephalum species were analyzed using eight ISSR primers. Results: A total of 128 bands were generated, of which 121 (94.3 %) were polymorphic. The highest polymorphism was observed with the IS7 and IS1 primers. The Polymorphism Information Content (PIC) values ranged from 0.286 (IS5) to 0.337 (IS1). Cluster analysis revealed distinct genetic groupings, with populations of D. kotschyi Boiss. (Isfahan), D. lindbergii Rech.f (Bojnourd), and D. moldavica L. (Urmia) forming separate clusters, indicating significant genetic differentiation. Conclusion: This study demonstrates the utility of ISSR markers in distinguishing Dracocephalum species and highlights the substantial genetic diversity within the genus. The high percentage of polymorphism underscores the rich genetic variability, which is essential for conservation efforts and potential medicinal applications. These findings provide valuable insights into the genetic structure of Dracocephalum species, laying the groundwork for future research in biotechnology and pharmaceutical development.
نوع مطالعه: پژوهشی |
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گياهان دارویی
دریافت: 1403/12/15 | پذیرش: 1405/2/6 | انتشار: 1405/2/16
دریافت: 1403/12/15 | پذیرش: 1405/2/6 | انتشار: 1405/2/16
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