Background: Depressive disorders are common, life-threatening and often disabling. Due to adverse effects of chemical antidepressant drugs, researcher’s attention has been shifted toward natural drug. In this work, the antidepressant effect of were evaluated against reserpine induced depression in mice Material and Methods: This experimental study, 42 male mice were randomly divided into 6 groups of 7 mice. Vehicle mice received normal saline (1 mg/kg, i.p.), negative and positive control groups received reserpine (5mg/kg, i.p.) and fluoxetine (20 mg/kg, i.p.) respectively and treatment groups received Kombucha tea at doses of 250, 500, 1000 mg/kg, 18 h after administration of reserpine. Mice were then tested in the forced swim and Rotarod tests. After behavioral test, blood sample were collected and used to assess blood antioxidant capacity Results: There was significant difference in the duration of immobility time between vehicle and reserpine treated groups (p<0.001). Administration of Kombucha tea at doses of 250, 500 and 1000 mg/kg into depressed mice significantly reduced the duration of immobility time. KT administration significantly improved blood antioxidant capacity of mice blood. Conclusion: These results provide support for the potential antidepressant effects of Kombucha tea against