سال 19، شماره 74 - ( 3-1399 )                   سال 19 شماره 74 صفحات 191-177 | برگشت به فهرست نسخه ها


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Sharifiyan F, Mirjalili S A, Fazilati M, Poorazizi E, Habibollahi S. Cytotoxic effect of hydro-alcoholic extract of pomegranate (Punica granatum L.) flowers and isolated ursolic acid on B16f10 melanoma cells. J. Med. Plants 2020; 19 (74) :177-191
URL: http://jmp.ir/article-1-2248-fa.html
شریفیان فاطمه، میرجلیلی عباس، فضیلتی محمد، پورعزیزی الهه، حبیب اللهی سعید. بررسی اثر سمیت سلولی عصاره هیدروالکلی گل‌های انار (Punica granatum L.) و اسید اورسولیک خالص ‌شده از آن بر روی سلول‌های ملانوما B16f10. فصلنامه گياهان دارویی. 1399; 19 (74) :177-191

URL: http://jmp.ir/article-1-2248-fa.html


1- گروه زیست شناسی، دانشگاه پیام نور، ایران
2- مرکزآموزش عالی امام خمینی(ره)،سازمان تحقیقات،آموزش وترویج کشاورزی،تهران،ایران ، abmirjalili@gmail.com
3- مرکزآموزش عالی امام خمینی(ره)،سازمان تحقیقات،آموزش وترویج کشاورزی،تهران،ایران
4- گروه بیوشیمی، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران
5- دانشکده شیمی، دانشگاه پیام نور، ایران
چکیده:   (3482 مشاهده)
مقدمه: ملانوما خطرناک‌ترین نوع سرطان پوست است و بیشترین سرعت رشد را در میان انواع سرطان‌ها داراست. اسید اورسولیک یکی از تری‌ترپنوئیدهای پنتاسیکلیک شناخته ‌شده است که به ‌طور گسترده در گیاهان دارویی از جمله گل انار وجود دارد. هدف: مطالعه حاضر به بررسی اثر سایتوتوکسیک عصاره اتانولی گل‌های انار و همچنین اسید اورسولیک خالص‌شده از آن بر سلول‌های ملانوما موشی B16f10 می‌پردازد. روش بررسی: خالص‌سازی اسید اورسولیک از گل‌های انار، با استفاده از عصاره‌گیری با کمک اولتراسوند و به دنبال آن فلش کروماتوگرافی فاز نرمال انجام شد. ساختار شیمیایی اسید اورسولیک خالص‌شده به‌وسیله کروماتوگرافی گازی جفت شده با اسپکتروسکوپی جرمی (GC-MS)، طیف‌سنجی مادون‌قرمز (IR) و 1H-NMR تأیید شد. سپس اثر عصاره اتانولی گل‌های انار و همچنین اسید اورسولیک خالص ‌شده از آن در مهار تکثیر سلول‌های ملانوما موشی B16f10 با استفاده از روش MTT انجام شد. نتایج: نتایج این مطالعه استخراج و خالص‌سازی موفق و پربازده اسید اورسولیک از گل‌های انار را توسط این روش فلش نشان داد. بعد از یک ‌بار فلش کروماتوگرافی، درصد خلوص اسید اورسولیک بیش از 97 درصد و مقدار محصول 1/0 درصد پودر خشک گل انار بود. تیمار سلول‌های B16f10 با عصاره‌ اتانولی گل‌های انار و اسید اورسولیک خالص‌شده از آن، فعالیت ضد‌تکثیری معنی‌داری را بعد از 48 و 72 ساعت نشان داد. این فعالیت مهاری از ‌عصاره گل‌های انار به‌صورت وابسته به غلظت و زمان مشاهده شد. نتیجه‌گیری: نتایج این تحقیق حاکی از ارزشمند بودن عصاره‌ گل‌های انار و اسید اورسولیک خالص‌شده از آن در پیشگیری و درمان ملانوما می‌باشد.
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نوع مطالعه: پژوهشی | موضوع مقاله: گياهان دارویی
دریافت: 1397/6/7 | پذیرش: 1398/1/17 | انتشار: 1399/4/31

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