year 15, Issue 59 (8-2016)                   J. Med. Plants 2016, 15(59): 145-152 | Back to browse issues page

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1- Faculty of Life Sciences and Computing, London Metropolitan University, London, UK
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- The Royal College of General Practitioners (RCGP), London, UK
4- Faculty of Life Sciences and Computing, London Metropolitan University, London, UK , Kenneth.white@londonmet.ac.uk
Abstract:   (2999 Views)
Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes.
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared.
Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested.
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.
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Type of Study: Research | Subject: Pharmacology & Toxicology
Received: 2016/02/20 | Accepted: 2016/04/20 | Published: 2016/10/3

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