سال 15، شماره 59 - ( English 1395 )
سال 15 شماره 59 صفحات 152-145 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Roostaei D, Rezazadeh S, Sharafi S, White K. Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens. J. Med. Plants 2016; 15 (59) :145-152
URL: http://jmp.ir/article-1-1474-fa.html
URL: http://jmp.ir/article-1-1474-fa.html
Roostaei D، Rezazadeh Sh، Sharafi S، White K. Isolation and Characterisation of Anti-diabetic Pharmacological Activities of Phytoestrogens. فصلنامه گياهان دارویی. 1395; 15 (59) :145-152
D Roostaei1 
، Sh Rezazadeh2 
، S Sharafi3 ، K White* 4
، Sh Rezazadeh2 ، S Sharafi3 ، K White* 4
1- Faculty of Life Sciences and Computing, London Metropolitan University, London, UK
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- The Royal College of General Practitioners (RCGP), London, UK
4- Faculty of Life Sciences and Computing, London Metropolitan University, London, UK ، Kenneth.white@londonmet.ac.uk
2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3- The Royal College of General Practitioners (RCGP), London, UK
4- Faculty of Life Sciences and Computing, London Metropolitan University, London, UK ، Kenneth.white@londonmet.ac.uk
چکیده: (3833 مشاهده)
Background: Isoflavones class of phytoestrogens including, genestein, daidzein and formononetin found in human dietary and show wide range of biological effects. These plant derived compounds have been shown to play a beneficial role in obesity and diabetes.
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared.
Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested.
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.
Objective: In this study the impact of these phytoestrogens on glucose uptake in HepG2 cell were compared.
Methods: Glucose uptake measurement was performed using 2-(N-(7-nitrobenzin-2-oxa-1, 3- diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) and Omega FluoStar plate reader. Incubation of cells (104/ml, in 24 well plate at 37 °C in 5% CO2 / air) with three phytoestrogens at concentration of 10-4 M to 10-9M in two studies mood, short term treatment (one hour) and long term treatment (24 hours) was tested.
Results: The data revealed, daidzein stimulates uptake of glucose, with a greater effect after a short treatment of one hour compared with treatment 24 hours. genistein exerted slightly inhibitory effect after one hour treatment compared with control, with the exception of treatment at 1 µM, which stimulated uptake about three-fold compared with control. Longer treatments with 10-4M to 10-6 M genistein resulted in gradual increase in glucose uptake to 2.4 times more than control, and thereafter a decline. A short treatment with formononetin inhibited glucose uptake, while longer treatments had variable effects, with an approximately two fold stimulation across a range of concentrations
Conclusion: Overall HepG2 cells showed a significant increase in glucose uptake after treatment with phytoestrogens compared to the control. There was significant difference in glucose uptake between short and long term treatments, as indicated by two-way ANOVA.
نوع مطالعه: پژوهشی |
موضوع مقاله:
فارماكولوژی و سم شناسی
دریافت: 1394/12/1 | پذیرش: 1395/2/1 | انتشار: 1395/7/12
دریافت: 1394/12/1 | پذیرش: 1395/2/1 | انتشار: 1395/7/12
ارسال پیام به نویسنده مسئول
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
