:: Volume 4, Issue 44 (12-2012) ::
JMP 2012, 4(44): 70-77 Back to browse issues page
Determination of Minimum Inhibitory Concentration (MIC) of Zataria multiflora Boiss. Essential Oil and Lysozim on L. monocytogenes
T Mohajerfar 1, A Hosseinzadeh 2, A Akhondzadeh Basti * 3, A Khanjari 1, A Misaghi 1, Gandomi Nasrabadi H (Ph.D.)
1- Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran
2- Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences
3- Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran , aakhond@ut.ac.ir
Abstract:   (4733 Views)
Background: Application of natural compounds, including essential oils (EOs) and lysozyme is an effective method against growth of bacterial pathogens in foods. Objective: Determination of minimum inhibitory concentration (MIC) of lysozyme and Zataria multiflora Boiss. essential oil on L. monocytogenes. Methods: In this study different concentrations of lysozyme and Zataria multiflora Boiss. EOwere used alone and in combination on BHI broth to determine MIC of Zataria multiflora Boiss. EO and lysozyme with macro dilution and micro dilution methods and effect of sub inhibitory concentrations of them on bacterial growth curve of L. monocytogenes. Results: The minimum inhibitory concentration (MIC) of Z. multiflora Boiss EO was estimated ٪0.04 using macro and microdilution. lysozyme at the highest concentration (1000 μg/ ml) was not effective in inhibition of bacterial growth and no MIC value obtained. Combination of EO and Lysozyme decreased the MIC value to %0.02 and 250μg/ mlfor Z. multifloraBoiss. EO and lysozyme, respectively. The results of growth curve analysis showed that combination was effective in increasing the lag phase. Conclusion: Z. multiflora Boiss and lysozyme showed to be effective against bacterial growth and its potential application in food systems may be suggested.
Keywords: Lysozym, Z. multiflora, Minimum inhibitory concentration (MIC)
Full-Text [PDF 213 kb]   (1376 Downloads)    
Type of Study: Research | Subject: Food Hygiene and Aquatic
Received: 2014/10/4 | Accepted: 2014/10/4 | Published: 2014/10/4

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Volume 4, Issue 44 (12-2012) Back to browse issues page