سال 12، شماره 47 - ( English 1392 )                   سال 12 شماره 47 صفحات 110-100 | برگشت به فهرست نسخه ها

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Qaderi A, Omidi M, Zebarjadi A, Hajiaghaee R. Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.). J. Med. Plants 2013; 12 (47) :100-110
URL: http://jmp.ir/article-1-83-fa.html
Qaderi A، Omidi M، Zebarjadi AR، Hajiaghaee R. Over - expression Effect of Gene Encoding 3-hydroxy-3-Methylglutaryl-CoA Reductase on Production of Taxol in Iranian Hazel (Corylus avellana L.). فصلنامه گياهان دارویی. 1392; 12 (47) :100-110

URL: http://jmp.ir/article-1-83-fa.html


1- Department of Plant Breeding, Science and Research Branch, Islamic Azad University ، Ardeshir582003@yahoo.com
2- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Tehran University
3- Agronomy and Plant Breeding Department, Faculty of Agriculture, Razi University
4- Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR
چکیده:   (5670 مشاهده)
Background: Sustainable and commercial production of taxol as an anti cancer drug is a critical point to its clinical application. Nowadays, hazel because of rapid growth and wide range distribution is considered as an alternative source of Taxol. Objective: To increase taxol production the cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) from Iranian hazel (GeneBank accession number KF306244, showed by CiHMGR) was isolated and over-expressed in pCAMBIA1304 binary vector. The effect of transient over-expression of HMGR in callus and leaf were evaluated on Taxol production. Methods: The calli was established through the culture of immature cotyledon on Murashige and Skoog basal medium supplemented with 2, 4-D and BA. The first strand cDNA of CiHMGR was synthesized by specific primers. Enzymatic assay of recombinant CiHMGR in E. coli were done by western blott and His-tag affinity techniques. Also production of taxol in transformed callus and leaf were evaluated by HPLC analysis. Results: An Open Reading Frame (ORF) with 1698 bp length and a deduced polypeptide with 566 amino acid residues were amplified. The highest and lowest amount of taxol was 0.016 mg/g.DW and 0.004 mg/gDW in transformed calli and untransformed leaves respectively. Conclusion: Generally the over-expression of HMGR increase the total isoprenoids yield, therefore to have high production of target secondary metabolites (taxol) we need both of network of transformed genes and elicited cell culture.
متن کامل [PDF 724 kb]   (1851 دریافت)    
نوع مطالعه: پژوهشی | موضوع مقاله: بيوتكنولوژی
دریافت: 1392/4/1 | پذیرش: 1392/4/19 | انتشار: 1392/7/30

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
CAPTCHA

ارسال پیام به نویسنده مسئول


بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به فصلنامه علمی پژوهشی گیاهان دارویی می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2024 CC BY-NC 4.0 | Journal of Medicinal Plants

Designed & Developed by : Yektaweb