en Changes in Essential Oil Composition and Leaf traits of Basil (<i>Ocimum basilicum</i> L.) Affected by Bio-stimulators / fertilizers Application كشاورزی و اتنوبوتانی Agriculture & Ethnobotany پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Basil (<em>Ocimum basilicum</em> L.), a member of the Lamiaceae family, has traditionally been used as a medicinal plant in the treatment of headaches, coughs, diarrhea, constipation, warts, worms, and kidney malfunctions. <strong>Objective:</strong> To investigate the foliar application effects of bio-stimulators and bio-fertilizers on morphological and phytochemical traits of basil (<em>Ocimum basilicum</em> L.). <strong>Methods:</strong> Bio-stimulators in three commercial formulations of aminolforte, kadostim and fosnutren (with concentration of 1.5 L.ha-1) through foliar application, and bio-fertilizers in three commercial formulations of nitroxin, super-nitro plus and barvar II (with concentration of 0.5 L.ha-1) through seed inoculation were considered as two studied factors. <strong>Results:</strong> The results showed that the interaction effect of bio-stimulators and bio - fertilizers was significant (p<0.01) on all of studied parameters except of chlorophyll content (SPAD value). The highest leaf fresh weight (25.47 g/plant) and leaf dry weight (6.48 g/plant) were obtained under fosnutren and nitroxin treatment, also maximum leaf number (206.33) was recorded in aminolforte and nitroxin treatment. The highest leaf area (1302.2 mm2/plant) was observed in kadostim and nitroxin treatment. Also results showed that the highest content of essential oil (0.43%) was obtained in aminolforte and nitroxin, methyl chavicol (37.13%) in fosnutren and super-nitro plus, geranial (29.05%) and caryophylene (6.66%) in kadostim and nitroxin, and carvacrol (31.60%) in fosnutren and nitroxin treated plants. <strong>Conclusion:</strong> In general, the best treatment to improve growth and phytochemical traits of <em>Ocimum basilicum</em> were kadostim&times;nitroxin and fosnutren&times;nitroxin.</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Basil (<em>Ocimum basilicum</em> L.), a member of the Lamiaceae family, has traditionally been used as a medicinal plant in the treatment of headaches, coughs, diarrhea, constipation, warts, worms, and kidney malfunctions. <strong>Objective:</strong> To investigate the foliar application effects of bio-stimulators and bio-fertilizers on morphological and phytochemical traits of basil (<em>Ocimum basilicum</em> L.). <strong>Methods:</strong> Bio-stimulators in three commercial formulations of aminolforte, kadostim and fosnutren (with concentration of 1.5 L.ha-1) through foliar application, and bio-fertilizers in three commercial formulations of nitroxin, super-nitro plus and barvar II (with concentration of 0.5 L.ha-1) through seed inoculation were considered as two studied factors. <strong>Results:</strong> The results showed that the interaction effect of bio-stimulators and bio - fertilizers was significant (p<0.01) on all of studied parameters except of chlorophyll content (SPAD value). The highest leaf fresh weight (25.47 g/plant) and leaf dry weight (6.48 g/plant) were obtained under fosnutren and nitroxin treatment, also maximum leaf number (206.33) was recorded in aminolforte and nitroxin treatment. The highest leaf area (1302.2 mm2/plant) was observed in kadostim and nitroxin treatment. Also results showed that the highest content of essential oil (0.43%) was obtained in aminolforte and nitroxin, methyl chavicol (37.13%) in fosnutren and super-nitro plus, geranial (29.05%) and caryophylene (6.66%) in kadostim and nitroxin, and carvacrol (31.60%) in fosnutren and nitroxin treated plants. <strong>Conclusion:</strong> In general, the best treatment to improve growth and phytochemical traits of <em>Ocimum basilicum</em> were kadostim&times;nitroxin and fosnutren&times;nitroxin.</div> Ocimum basilicum L., Bio-fertilizers, Bio - stimulators, Essential Oil, Leaf traits Ocimum basilicum L., Bio-fertilizers, Bio - stimulators, Essential Oil, Leaf traits 83 92 http://jmp.ir/browse.php?a_code=A-10-44-21&slc_lang=en&sid=1 A Rahimi Shokooh Rahimi Shokooh 100319475328460042062 100319475328460042062 No Department of Horticulture, Islamic Azad University, Science and Research Branch A MR Dehghani-Meshkani MR Dehghani-Meshkani 100319475328460042063 100319475328460042063 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR A Mehrafarin A Mehrafarin 100319475328460042064 100319475328460042064 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR F Khalighi-sigaroodi F Khalighi-sigaroodi 100319475328460042065 100319475328460042065 No Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Pharmacognosy & Pharmaceutics Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR H Naghdi Badi H Naghdi Badi Naghdibadi@yahoo.com 100319475328460042066 100319475328460042066 Yes Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR