en Determination of Tocopherols and Fatty Acids in Seeds of <i>Silybum marianum</i> (L.) Gaerth فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> The dried fruits of <i>Silybum marianum </i>(L.) Gaertn (Compositae) contain silymarin, an isomeric mixture of flavonolignans. Silymarin acts as a strong anti -hepatotoxic. This fruits contains a high amount of oil .</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> The objective of this study was to determine total lipid content, fatty acid composition and content of different kind of tocopherols in seed oils of <i>S. marianum</i>.</p> <p dir="ltr" style="text-align: justify;"><strong>Method:</strong> Fatty acid compositon and a -, &gamma;- and &delta;-tocopherol (vitamin E) content from seeds of <i>Silybum</i> <i>maianum</i> were investigated by gas chromatography and high performance liquid chromatography methods.</p> <div dir="ltr" style="text-align: justify;"><strong>Results and Conclusion:</strong> Total lipid percentage was approximately 25% and nine fatty acids including palmitic acid (8.25 %), palmeotic acid (0.07 %), Stearic acid (6.67 %), oleic acid (31.58 %), Isomer oleic acid (0.53 %), linoleic acid (45.36 %), linolenic acid (0.87 %), arashidic acid (4.11 %), eicozantoic acid (0.088 %) and behenic acid (2.6 %) were determined. a -, &gamma;- and &delta; -tocopherol content were around 563.157, 88.87 and 163.791 mg Kg ^-1DW respectively that could be a natural good sources of antioxidant.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> The dried fruits of <i>Silybum marianum </i>(L.) Gaertn (Compositae) contain silymarin, an isomeric mixture of flavonolignans. Silymarin acts as a strong anti -hepatotoxic. This fruits contains a high amount of oil .</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> The objective of this study was to determine total lipid content, fatty acid composition and content of different kind of tocopherols in seed oils of <i>S. marianum</i>.</p> <p dir="ltr" style="text-align: justify;"><strong>Method:</strong> Fatty acid compositon and a -, &gamma;- and &delta;-tocopherol (vitamin E) content from seeds of <i>Silybum</i> <i>maianum</i> were investigated by gas chromatography and high performance liquid chromatography methods.</p> <div dir="ltr" style="text-align: justify;"><strong>Results and Conclusion:</strong> Total lipid percentage was approximately 25% and nine fatty acids including palmitic acid (8.25 %), palmeotic acid (0.07 %), Stearic acid (6.67 %), oleic acid (31.58 %), Isomer oleic acid (0.53 %), linoleic acid (45.36 %), linolenic acid (0.87 %), arashidic acid (4.11 %), eicozantoic acid (0.088 %) and behenic acid (2.6 %) were determined. a -, &gamma;- and &delta; -tocopherol content were around 563.157, 88.87 and 163.791 mg Kg ^-1DW respectively that could be a natural good sources of antioxidant.</div> Silybum marianum, Fatty acid, Tocopherol, GC, HPLC Silybum marianum, Fatty acid, Tocopherol, GC, HPLC 69 76 http://jmp.ir/browse.php?a_code=A-10-74-6&slc_lang=en&sid=1 T Hasanloo T Hasanloo thasanloo@yahoo.com 100319475328460038864 100319475328460038864 Yes Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, Karaj Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, Karaj M Bahmanei M Bahmanei 100319475328460038865 100319475328460038865 No R&D labs, Savola Behshahr Ind. Co., 8 th. Km. Fath Highway, Tehran R&D labs, Savola Behshahr Ind. Co., 8 th. Km. Fath Highway, Tehran R Sepehrifar R Sepehrifar 100319475328460038866 100319475328460038866 No Department of Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, Karaj Department of Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, Karaj F Kalantari F Kalantari 100319475328460038867 100319475328460038867 No R&D labs, Savola Behshahr Ind. Co., 8 th. Km. Fath Highway, Tehran R&D labs, Savola Behshahr Ind. Co., 8 th. Km. Fath Highway, Tehran