en Trypanocidal Activity of Some Medicinal Plants Against the Epimastigotes of <i>Trypanosoma cruzi</i> فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Some of medicinal plants are a potential source of new drugs to improve the treatment of Chagase disease whose treatment is still a challenge. Here in this screening, the <i>in vitro</i> trypanocidal activity of some fractions for 16 medicinal plants, collected from the northern part of Iran, has been reported.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Aerial parts of the plants were dried carefully and followed by extraction with hexane and methanol, successively, by maceration at room temperature. Different concentrations of the plant extracts in ethanol were investigated against the epimastigotes of <i>T. cruzi</i>. The movement of epimastigotes was observed under a microscope. We assumed that immobilized organisms were died. The negative control contained ethanol in the same proportion utilized to dissolve the drugs. Each assay was performed in duplicate together with gentian violet as a positive control.</p> <p dir="ltr" style="text-align: justify;"><strong>Results: </strong>Results show that hexane extracts of <i>Rubus hyrcanus</i> and <i>Salvia sclerae </i>have been observed the most activity against the epimastigotes of <i>T. cruzi </i>(MLC = 12.5 m g/ml). None of methanol fractions shows trypanocidal activity except <i>Salvia sclerae </i>(MLC =<br> 50 m g/ml)<i>.</i> <i>Echium amoenum, Satureja macrantha, S. atropatana </i>and <i>Stachys laxa</i> did not display activity lower than 100 m g/ml in both hexane and methanol extracts.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> Some of Iranian medicinal plants (<i>Salvia sclerae, Marrubium vulgare</i> and <i>Rubus hyrcanus</i>) could be the promising source of active components against the epimastigotes of <i>T. cruzi</i> and need to further phytochemical and pharmacological studies.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Some of medicinal plants are a potential source of new drugs to improve the treatment of Chagase disease whose treatment is still a challenge. Here in this screening, the <i>in vitro</i> trypanocidal activity of some fractions for 16 medicinal plants, collected from the northern part of Iran, has been reported.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Aerial parts of the plants were dried carefully and followed by extraction with hexane and methanol, successively, by maceration at room temperature. Different concentrations of the plant extracts in ethanol were investigated against the epimastigotes of <i>T. cruzi</i>. The movement of epimastigotes was observed under a microscope. We assumed that immobilized organisms were died. The negative control contained ethanol in the same proportion utilized to dissolve the drugs. Each assay was performed in duplicate together with gentian violet as a positive control.</p> <p dir="ltr" style="text-align: justify;"><strong>Results: </strong>Results show that hexane extracts of <i>Rubus hyrcanus</i> and <i>Salvia sclerae </i>have been observed the most activity against the epimastigotes of <i>T. cruzi </i>(MLC = 12.5 m g/ml). None of methanol fractions shows trypanocidal activity except <i>Salvia sclerae </i>(MLC =<br> 50 m g/ml)<i>.</i> <i>Echium amoenum, Satureja macrantha, S. atropatana </i>and <i>Stachys laxa</i> did not display activity lower than 100 m g/ml in both hexane and methanol extracts.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> Some of Iranian medicinal plants (<i>Salvia sclerae, Marrubium vulgare</i> and <i>Rubus hyrcanus</i>) could be the promising source of active components against the epimastigotes of <i>T. cruzi</i> and need to further phytochemical and pharmacological studies.</div> Trypanocidal activity, Salvia sclerae, Rubus hyrcanus Trypanocidal activity, Salvia sclerae, Rubus hyrcanus 44 48 http://jmp.ir/browse.php?a_code=A-10-161-5&slc_lang=en&sid=1 AR Gohari AR Gohari 100319475328460038835 100319475328460038835 No Medicinal Plants Research Center, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran Medicinal Plants Research Center, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran S Saeidnia S Saeidnia soodabehsaeidnia@hotmail.com 100319475328460038836 100319475328460038836 Yes Medicinal Plants Research Center, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran Medicinal Plants Research Center, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran A Hadjiakhoondi A Hadjiakhoondi 100319475328460038837 100319475328460038837 No Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences/ University of Tehran, Tehran A Naghinejad A Naghinejad 100319475328460038838 100319475328460038838 No Department of Botany, Faculty of Sciences, University of Tehran, Tehran Department of Botany, Faculty of Sciences, University of Tehran, Tehran T Yagura T Yagura 100319475328460038839 100319475328460038839 No Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan