en Phytoprevention of aflatoxin production فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Consumption of mycotoxic foods is associated with several cases of human poisoning, or mycotoxicosis, sometimes resulting in death.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> Phytopreventive inhibition of <i>Aspergillus parasiticus</i> growth and its aflatoxin production by the essential oils extracted from <i>Thymus kotschyanus </i>Boiss & Hohen<i>&nbsp;</i>and <i>Zataria multiflora </i>Boiss<i>.</i> is reported in this study.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Minimal inhibitory concentration (MIC), minimal fungicidal concentration (MFC) and fungicidal kinetics of the oils were determined and compared with each other. Results: The oils from the above mentioned plants were found to be strongly fungicidal and inhibitory to aflatoxin production. Both oils inhibited aflatoxin B₁ (AFB₁) production by <i>A.parasiticus</i>. <i>T. kotschyanus </i>and <i>Z. multiflora </i>oils<i>&nbsp;</i>at 25 ppm concentration, reduced AFB₁ levels by 100% and 47.87% respectively. Aflatoxin production was significantly inhibited at lower than fungistatic concentration of both oils. The analysis of oils by GC and GC/MS led to identification of 27 and 22 components in <i>T. kotschyanus </i>and <i>Z. multiflora </i>Boiss<i>. </i>respectively which were very similar to each other.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion: </strong>Prevention of fungal growth and aflatoxin production by natural compounds is recommended.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Consumption of mycotoxic foods is associated with several cases of human poisoning, or mycotoxicosis, sometimes resulting in death.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> Phytopreventive inhibition of <i>Aspergillus parasiticus</i> growth and its aflatoxin production by the essential oils extracted from <i>Thymus kotschyanus </i>Boiss & Hohen<i>&nbsp;</i>and <i>Zataria multiflora </i>Boiss<i>.</i> is reported in this study.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Minimal inhibitory concentration (MIC), minimal fungicidal concentration (MFC) and fungicidal kinetics of the oils were determined and compared with each other. Results: The oils from the above mentioned plants were found to be strongly fungicidal and inhibitory to aflatoxin production. Both oils inhibited aflatoxin B₁ (AFB₁) production by <i>A.parasiticus</i>. <i>T. kotschyanus </i>and <i>Z. multiflora </i>oils<i>&nbsp;</i>at 25 ppm concentration, reduced AFB₁ levels by 100% and 47.87% respectively. Aflatoxin production was significantly inhibited at lower than fungistatic concentration of both oils. The analysis of oils by GC and GC/MS led to identification of 27 and 22 components in <i>T. kotschyanus </i>and <i>Z. multiflora </i>Boiss<i>. </i>respectively which were very similar to each other.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion: </strong>Prevention of fungal growth and aflatoxin production by natural compounds is recommended.</div> <,i>,Thymus kotschyanus<,/i>,, <,i>,Zataria multiflora<,/i>,, Essential oil, Antifungal, Aflatoxin <,i>,Thymus kotschyanus<,/i>,, <,i>,Zataria multiflora<,/i>,, Essential oil, Antifungal, Aflatoxin 97 104 http://jmp.ir/browse.php?a_code=A-10-179-3&slc_lang=en&sid=1 I Rasooli I Rasooli 100319475328460038564 100319475328460038564 Yes Medicinal Plants Research Center, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran - 3319118651, Iran Medicinal Plants Research Center, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran - 3319118651, Iran MH Fakoor MH Fakoor 100319475328460038565 100319475328460038565 No Medicinal Plants Research Center, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran - 3319118651, Iran Medicinal Plants Research Center, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's shrine, Tehran - 3319118651, Iran AA Allameh AA Allameh 100319475328460038566 100319475328460038566 No Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran MB Rezaee MB Rezaee 100319475328460038567 100319475328460038567 No Department of Medicinal Plants, Institute for Research in Forests and Rangelands, Tehran, Iran Department of Medicinal Plants, Institute for Research in Forests and Rangelands, Tehran, Iran P Owlia P Owlia 100319475328460038568 100319475328460038568 No School of Medicine, Shahed University, Tehran, Iran School of Medicine, Shahed University, Tehran, Iran