en Isolation and Quantificative Analysis of Oleanolic Acid from <i>Satureja mutica</i> Fisch. & C. A. Mey. فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Satureja mutica</i> (Lamiaceae) is an herbaceous plant which grows in North-West of Iran. Oleanolic acid has been previously isolated from some Lamiaceae genus and showed a broad range of biological activities. Besides <i>Silphium</i> spp., <i>Panax quinquefolium</i> root and <i>Calendula officinalis</i> flower as the interesting source of oleanolic acid used in the herbal industry, here we report the isolation and quantitative evaluation of oleanolic acid, as one of the major constituents in <i>S. mutica. </i></p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Dried aerial parts of <i>S. mutica</i> were successively extracted with diethyl ether. This extract was used for further isolation on silica gel column chromatography to obtain compound 1. Isolated compound was identified based on the spectral data of ¹H- NMR, ¹³C-NMR and Mass spectroscopy. Densitometric analysis of the developed plate of TLC was carried out to quantify the oleanolic acid using TLC scanner.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compound (1) was structural elucidated as oleanolic acid and its NMR data showed good agreement with the reference data mentioned in literature. Spectrodensitometric analysis showed that the band characterized by absorption maximum of oleanolic acid was placed at &lambda;max = 270 nm without derivatization. The contents of oleanolic acid are calculated 17.5 mg in 100 g of <i>S. mutica</i> leaves based on dry weight.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> Oleanolic acid is a ubiquitous triterpenoid in plant kingdom, medicinal herbs, and is integral part of the human diet. Densitometric analysis is the accurate, selective, and precise method which can be used for routine quality control analysis and quantitative determination of oleanolic acid in Lamiaceae plants specially <i>Satureja </i>species, as one of the industrial source of this compound.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Satureja mutica</i> (Lamiaceae) is an herbaceous plant which grows in North-West of Iran. Oleanolic acid has been previously isolated from some Lamiaceae genus and showed a broad range of biological activities. Besides <i>Silphium</i> spp., <i>Panax quinquefolium</i> root and <i>Calendula officinalis</i> flower as the interesting source of oleanolic acid used in the herbal industry, here we report the isolation and quantitative evaluation of oleanolic acid, as one of the major constituents in <i>S. mutica. </i></p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Dried aerial parts of <i>S. mutica</i> were successively extracted with diethyl ether. This extract was used for further isolation on silica gel column chromatography to obtain compound 1. Isolated compound was identified based on the spectral data of ¹H- NMR, ¹³C-NMR and Mass spectroscopy. Densitometric analysis of the developed plate of TLC was carried out to quantify the oleanolic acid using TLC scanner.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compound (1) was structural elucidated as oleanolic acid and its NMR data showed good agreement with the reference data mentioned in literature. Spectrodensitometric analysis showed that the band characterized by absorption maximum of oleanolic acid was placed at &lambda;max = 270 nm without derivatization. The contents of oleanolic acid are calculated 17.5 mg in 100 g of <i>S. mutica</i> leaves based on dry weight.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> Oleanolic acid is a ubiquitous triterpenoid in plant kingdom, medicinal herbs, and is integral part of the human diet. Densitometric analysis is the accurate, selective, and precise method which can be used for routine quality control analysis and quantitative determination of oleanolic acid in Lamiaceae plants specially <i>Satureja </i>species, as one of the industrial source of this compound.</div> Lamiaceae, <,i>,Satureja mutica<,/i>,, Oleanolic acid, TLC scanner Lamiaceae, <,i>,Satureja mutica<,/i>,, Oleanolic acid, TLC scanner 65 69 http://jmp.ir/browse.php?a_code=A-10-175-5&slc_lang=en&sid=1 AR Gohari AR Gohari goharii@yahoo.com 100319475328460038540 100319475328460038540 Yes Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran S Saeidnia S Saeidnia 100319475328460038541 100319475328460038541 No Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran A Hadjiakhoondi A Hadjiakhoondi 100319475328460038542 100319475328460038542 No Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran M Abdoullahi M Abdoullahi 100319475328460038543 100319475328460038543 No Department of Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran Department of Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran M Nezafati M Nezafati 100319475328460038544 100319475328460038544 No Faculty of Pharmacy, Islamic Azad University, Tehran Faculty of Pharmacy, Islamic Azad University, Tehran