en Protective Effects of Quercetin on Spermatogenesis in Streptozotocin-induced Diabetic Rat فارماكولوژی و سم شناسی Pharmacology & Toxicology پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily (IP). Diabetes was induced by a single intra peritonea injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg (IP) Quercetin (p<0.05) in comparison to control and experimental groups.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> Since in our study 15 mg/kg (IP) Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Quercetin is a strong antioxidant and long-term treatment of STZ-diabetic animals and it has been shown to reduce oxidative stress.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Wistar male rat (n=40) were allocated into three groups, control group (n=10) and Quercetin (QR) group that received 15mg/kg (IP) QR, (n=10), and Diabetic group that received 55 mg/kg (IP) streptozotocin (STZ) (n=20) which was subdivided to two groups of 10 STZ group and treatment group. Treatment group received 55 mg/kg (IP) STZ plus15mg/kg QR, daily for,4 weeks, respectively however, the control group just received an equal volume of distilled water daily (IP). Diabetes was induced by a single intra peritonea injection of streptozotocin (55 mg/kg). Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg (IP) Quercetin (p<0.05) in comparison to control and experimental groups.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> Since in our study 15 mg/kg (IP) Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species (ROS) in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat.</div> Quercetin, Streptozotocin, Spermatogenesis, Rat Quercetin, Streptozotocin, Spermatogenesis, Rat 57 64 http://jmp.ir/browse.php?a_code=A-10-176-10&slc_lang=en&sid=1 A Khaki A Khaki arashkhaki@yahoo.com 100319475328460038534 100319475328460038534 Yes Department of Veterinary Pathology (YRC), Islamic Azad University Tabriz Branch Department of Veterinary Pathology (YRC), Islamic Azad University Tabriz Branch M Nouri M Nouri 100319475328460038535 100319475328460038535 No Department of Biochemistry, Tabriz University of Medical Sciences, Iran Department of Biochemistry, Tabriz University of Medical Sciences, Iran F Fathiazad F Fathiazad 100319475328460038536 100319475328460038536 No Department of Pharmacognosy Tabriz University of Medical Sciences, Iran Department of Pharmacognosy Tabriz University of Medical Sciences, Iran HR Ahmadi-Ashtiani HR Ahmadi-Ashtiani 100319475328460038537 100319475328460038537 No Clinical Biochemistry, Tarbiat-e-Modarres University, School of Medical Science, Department of Clinical Biochemistry, Tehran, Iran and Biochemistry & Nutrition Department of Zanjan Medical University Clinical Biochemistry, Tarbiat-e-Modarres University, School of Medical Science, Department of Clinical Biochemistry, Tehran, Iran and Biochemistry & Nutrition Department of Zanjan Medical University H Rastgar H Rastgar 100319475328460038538 100319475328460038538 No Ministry of Health and Medical Education, Food & Drug Laboratory Research center, Tehran, Iran Ministry of Health and Medical Education, Food & Drug Laboratory Research center, Tehran, Iran Sh Rezazadeh Sh Rezazadeh 100319475328460038539 100319475328460038539 No Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal Plants, ACECR, Tehran, Iran Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal Plants, ACECR, Tehran, Iran