en Cytotoxic Flavonoid from <i>Achillea talagonica</i> Bioss. فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Achillea talagonica </i>(Asteraceae) is an endemic herbaceous plant in Iran with small yellow flowers and several times pinnately divided leaves in worm shape. The plant was found to be cytotoxic in our previous studies.</p> <p dir="ltr" style="text-align: justify;"><strong>Materials and Methods:</strong> <i>A. talagonica</i> was collected in May 2005 from Talegan in north of Iran during the full flowering stage. Dried aerial parts of the plant were reduced in to small pieces and percolated with ethyl acetate for 72 hours. The extract obtained was filtered and then concentrated under reduced pressure and completely dried by a freeze dryer. Column chromatography of the extract on silica gel and sephadex LH-20 yielded in isolation of three main components. Cytotoxic activity was evaluated against the <i>Artemia salina</i> larvae by the Brine Shrimp Cytotoxicity Bioassay.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compounds were identified as &beta;-sitosterol (1), salvigenin (5- hydroxy 4&#39;, 6, 7- three methoxy flavone) (2) and santoflavon (5- hydroxy 4&#39;, 3&#39;, 6, 7- tetra methoxy flavone) (3). NMR data of all the isolated compounds showed good agreement with literature data.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> In our previous studies ethyl acetate extract of <i>A. talagonica</i> showed cytotoxic activity in brine shrimp lethality assay. The results of BST assay indicated that only santoflavone (3) showed a good cytotoxic activity (LC₅₀= 15 &mu; g/ml) against <i>A. salina</i> larvae so this compound seems to be responsible for the extract toxicity of <i>A. talagonica</i> against <i>Artemia</i> nauplii.</div> <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> <i>Achillea talagonica </i>(Asteraceae) is an endemic herbaceous plant in Iran with small yellow flowers and several times pinnately divided leaves in worm shape. The plant was found to be cytotoxic in our previous studies.</p> <p dir="ltr" style="text-align: justify;"><strong>Materials and Methods:</strong> <i>A. talagonica</i> was collected in May 2005 from Talegan in north of Iran during the full flowering stage. Dried aerial parts of the plant were reduced in to small pieces and percolated with ethyl acetate for 72 hours. The extract obtained was filtered and then concentrated under reduced pressure and completely dried by a freeze dryer. Column chromatography of the extract on silica gel and sephadex LH-20 yielded in isolation of three main components. Cytotoxic activity was evaluated against the <i>Artemia salina</i> larvae by the Brine Shrimp Cytotoxicity Bioassay.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Isolated compounds were identified as &beta;-sitosterol (1), salvigenin (5- hydroxy 4&#39;, 6, 7- three methoxy flavone) (2) and santoflavon (5- hydroxy 4&#39;, 3&#39;, 6, 7- tetra methoxy flavone) (3). NMR data of all the isolated compounds showed good agreement with literature data.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusions:</strong> In our previous studies ethyl acetate extract of <i>A. talagonica</i> showed cytotoxic activity in brine shrimp lethality assay. The results of BST assay indicated that only santoflavone (3) showed a good cytotoxic activity (LC₅₀= 15 &mu; g/ml) against <i>A. salina</i> larvae so this compound seems to be responsible for the extract toxicity of <i>A. talagonica</i> against <i>Artemia</i> nauplii.</div> Asteraceae, <,i>,Achillea talagonica<,/i>,, Santoflavone, Salvigenin, Cytotoxic Asteraceae, <,i>,Achillea talagonica<,/i>,, Santoflavone, Salvigenin, Cytotoxic 52 56 http://jmp.ir/browse.php?a_code=A-10-161-4&slc_lang=en&sid=1 S Saeidnia S Saeidnia soodabehsaeidnia@hotmail.com 100319475328460038530 100319475328460038530 Yes Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran F Moradi-Afrapoli F Moradi-Afrapoli 100319475328460038531 100319475328460038531 No Department of Pharmacognosy, Faculty of Pharmacy, Medicinal Sciences, University of Tehran, Tehran Department of Pharmacognosy, Faculty of Pharmacy, Medicinal Sciences, University of Tehran, Tehran AR Gohari AR Gohari 100319475328460038532 100319475328460038532 No Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran M Malmir M Malmir 100319475328460038533 100319475328460038533 No Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran, Medicinal plants Research center, Medicinal Sciences, University of Tehran, Tehran,