en Classification of Iranian Garlic (<i>Allium sativum</i> L.) Ecotypes Using RAPD Marker بيوتكنولوژی Biotechnology پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background: </strong>Garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. The analysis of genetic diversity plays an important role in breeding programs. The RAPD technique could be very effective in detecting genetic variation in garlic.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> The objective of the present work was to detect molecular polymorphism among Iranian garlic ecotypes by RAPD technique.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Random Amplified Polymorphic DNA (RAPD) marker utilized to assess the genetic diversity among twenty-two different ecotypes of the Iranian garlic. Genomic DNA was extracted from young leaves using Dellaporta method. RAPD amplification was performed using ten 10-mers arbitrary primers.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Results indicated that five out of ten pair primers had no amplification. A total of 35 RAPD bands were produced, 31 of which (88.5%) were polymorphic. The similarity matrices and dendrogram were obtained using UPGMA algorithms.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> In conclusion the entire population was grouped into four clusters with 3, 9, 1 and 9 ecotypes. No significant relationship between genetic diversity detected by RAPD technique and geographical origins.</div> <p dir="ltr" style="text-align: justify;"><strong>Background: </strong>Garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. The analysis of genetic diversity plays an important role in breeding programs. The RAPD technique could be very effective in detecting genetic variation in garlic.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> The objective of the present work was to detect molecular polymorphism among Iranian garlic ecotypes by RAPD technique.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Random Amplified Polymorphic DNA (RAPD) marker utilized to assess the genetic diversity among twenty-two different ecotypes of the Iranian garlic. Genomic DNA was extracted from young leaves using Dellaporta method. RAPD amplification was performed using ten 10-mers arbitrary primers.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> Results indicated that five out of ten pair primers had no amplification. A total of 35 RAPD bands were produced, 31 of which (88.5%) were polymorphic. The similarity matrices and dendrogram were obtained using UPGMA algorithms.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> In conclusion the entire population was grouped into four clusters with 3, 9, 1 and 9 ecotypes. No significant relationship between genetic diversity detected by RAPD technique and geographical origins.</div> <,i>,Allium sativum<,/i>, L., Ecotype, Genetic diversity, Polymorphism, RAPD marker, UPGMA <,i>,Allium sativum<,/i>, L., Ecotype, Genetic diversity, Polymorphism, RAPD marker, UPGMA 45 51 http://jmp.ir/browse.php?a_code=A-10-417-1&slc_lang=en&sid=1 M Abdoli M Abdoli abdoli_m@yahoo.com 100319475328460038524 100319475328460038524 Yes Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj B Habibi-Khaniani B Habibi-Khaniani 100319475328460038525 100319475328460038525 No Department of Agronomy and Plant Breeding, Islamic Azad University of Dezful Department of Agronomy and Plant Breeding, Islamic Azad University of Dezful K Baghalian K Baghalian 100319475328460038526 100319475328460038526 No Department of Horticulture, Islamic Azad University- Karaj Branch Department of Horticulture, Islamic Azad University- Karaj Branch S Shahnazi S Shahnazi 100319475328460038527 100319475328460038527 No Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj H Rassouli H Rassouli 100319475328460038528 100319475328460038528 No Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj H Naghdi Badi H Naghdi Badi 100319475328460038529 100319475328460038529 No Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj Institute of Medicinal Plants, Iranian Academic Center for Education, Culture and Research (ACECR), Karaj