Journal of Medicinal Plants

en Antioxidant Activity, Total Phenol and Total Anthocyanin Contents of Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv. Antioxidant Activity, Total Phenol and Total Anthocyanin Contents of Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv. فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;">Background: Cornus sanguinea L subsp australis (C.A. Mey.) Jáv. (Cornaceae) is a native species in north and northwest of Iran. It is locally named Siah-al. The genus Cornus is rich source of anthocyanins. Objective: In this study the antioxidant activity, total phenol and total anthocyanin contents of different extracts of C. sanguinea L subsp australis. were investigated for the first time. Methods: The fruits were extracted with ethyl acetate, methanol (1% HCl) and water. DPPH and FRAP assay were performed for investigation of antioxidant activity of each extract. The total phenols were measured by Folin-Ciocalteu method while total anthocyanins were detected by spectroscopic method modified by Peksel. Results: The results showed that C. sanguinea L subsp australis methanol (1% HCl) extract (CME) had the highest amount of anthocyanins (12.56 ± 0.01 µmol/g extract) as well as the highest amount of total phenolics (88.56 ± 0.04 mg GAE/ g dry extract). The CME were found to have the highest antioxidant activity in DPPH assay (IC50=90.43 µg/ml) and in FRAP method (1419.167± 0.025mmol FeII / g dry extract). Radical scavenger activity of CME at 100 μg/ml was comparable with α-tocopherol (20 μg/ml) and with BHA (200 μg/ml), p>0.05. Conclusion: There was a significant correlation between the total phenolic content an antioxidant activity of CME as well as total anthocyanin and antioxidant activity in DPPH assay (R2 = 0.99). The results suggest that C. sanguinea L subsp australis is a natural sources of anthocyanin and have considerable antioxidant activity.</div> <div dir="ltr" style="text-align: justify;">Background: Cornus sanguinea L subsp australis (C.A. Mey.) Jáv. (Cornaceae) is a native species in north and northwest of Iran. It is locally named Siah-al. The genus Cornus is rich source of anthocyanins. Objective: In this study the antioxidant activity, total phenol and total anthocyanin contents of different extracts of C. sanguinea L subsp australis. were investigated for the first time. Methods: The fruits were extracted with ethyl acetate, methanol (1% HCl) and water. DPPH and FRAP assay were performed for investigation of antioxidant activity of each extract. The total phenols were measured by Folin-Ciocalteu method while total anthocyanins were detected by spectroscopic method modified by Peksel. Results: The results showed that C. sanguinea L subsp australis methanol (1% HCl) extract (CME) had the highest amount of anthocyanins (12.56 ± 0.01 µmol/g extract) as well as the highest amount of total phenolics (88.56 ± 0.04 mg GAE/ g dry extract). The CME were found to have the highest antioxidant activity in DPPH assay (IC50=90.43 µg/ml) and in FRAP method (1419.167± 0.025mmol FeII / g dry extract). Radical scavenger activity of CME at 100 μg/ml was comparable with α-tocopherol (20 μg/ml) and with BHA (200 μg/ml), p>0.05. Conclusion: There was a significant correlation between the total phenolic content an antioxidant activity of CME as well as total anthocyanin and antioxidant activity in DPPH assay (R2 = 0.99). The results suggest that C. sanguinea L subsp australis is a natural sources of anthocyanin and have considerable antioxidant activity.</div> ,Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv., Antioxidant, Total anthocyanin, Total phenol Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv., Antioxidant, Total anthocyanin, Total phenol 69 74 http://jmp.ir/browse.php?a_code=A-10-70-1&slc_lang=en&sid=1 F Yousfbeyk F Yousfbeyk 100319475328460041944 100319475328460041944 No Guilan University of Medical Sciences Guilan University of Medical Sciences T Esmaiili T Esmaiili 100319475328460041945 100319475328460041945 No Tehran University of Medical Sciences Tehran University of Medical Sciences Z Pashna Z Pashna 100319475328460041946 100319475328460041946 No Tehran University of Medical Sciences Tehran University of Medical Sciences Z Hozori Z Hozori 100319475328460041947 100319475328460041947 No Tehran University of Medical Sciences Tehran University of Medical Sciences AR Ghohari AR Ghohari 100319475328460041948 100319475328460041948 No Tehran University of Medical Sciences Tehran University of Medical Sciences SN Ostad SN Ostad 100319475328460041949 100319475328460041949 No Tehran University of Medical Sciences Tehran University of Medical Sciences GhR Amin GhR Amin amin@tums.ac.ir 100319475328460041950 100319475328460041950 Yes Tehran University of Medical Sciences Tehran University of Medical Sciences