en Effects of Sub-inhibitory Concentrations of Essential Oils of <i>Mentha spicata</i> and <i>Cumminum cyminum</i> on Virulence Factors of <i>Pseudomonas aeruginosa</i> فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;"> <p style="text-align: justify;"><strong>Background:</strong> <i>Pseudomonas aeruginosa</i> is one of important opportunistic pathogen, that cases serious infections . It produces many virulence factors, and this bacterium usually is resistance against antimicrobial agents.</p> <p style="text-align: justify;"><strong>Objective:</strong> The aim of this study was evaluate the effects of sub-MICs of essential oils of <i>Mentha spicata </i>and<i> Cumminum cyminum</i> on alginate production, biofilm formation, swimming, twitching and adhesion in<i> P. aeruginosa </i>8821M.</p> <p style="text-align: justify;"><strong>Method:</strong> Minimal inhibitory concentrations (MIC) of essential oils of <i>Mentha spicata </i>and<i> Cumminum cyminum</i> were determined by macrodilution method. Alginate production, biofilm formation, swimming, twitching and adhesion in the present of sub-MICs (1/2, 1/4 and 1/8 MIC) of essential oils were determined in mucoid <i>P. aeruginosa </i>8821M and compared with controls.</p> <p style="text-align: justify;"><strong>Results:</strong> The MICs of essential oils against <i>P. aeruginosa</i> for<i>&nbsp;M. spicata </i>and<i> C. cyminum</i> <i></i>oils were obtained 16 and 32 &mu;g/ml respectively. The results show that all oils at 1/2 and 1/4 MICs were significantly reduced all tested virulence factors. At 1/8 MICs, M. spicata had effect just on adhesion but <i><i>C. cyminum </i></i>had effect on&nbsp;Alginate production, biofilm formation, swimming and twitching . <i></i></p> <div style="text-align: justify;"><strong>Conclusion:</strong><i> T</i>his study showed that sub-MIC levels of <i><i>M. spicata </i></i>and <i>C. cyminum </i>essential oils affected alginate production, biofilm formation, swimming, twitching and adhesion in<i> <i>P. aeruginosa</i> </i>8821M and it is probable to use of these medicinal plants for treating.</div> </div> <p style="text-align: justify;"><strong>Background:</strong> <i>Pseudomonas aeruginosa</i> is one of important opportunistic pathogen, that cases serious infections . It produces many virulence factors, and this bacterium usually is resistance against antimicrobial agents.</p> <p style="text-align: justify;"><strong>Objective:</strong> The aim of this study was evaluate the effects of sub-MICs of essential oils of <i>Mentha spicata </i>and<i> Cumminum cyminum</i> on alginate production, biofilm formation, swimming, twitching and adhesion in<i> P. aeruginosa </i>8821M.</p> <p style="text-align: justify;"><strong>Method:</strong> Minimal inhibitory concentrations (MIC) of essential oils of <i>Mentha spicata </i>and<i> Cumminum cyminum</i> were determined by macrodilution method. Alginate production, biofilm formation, swimming, twitching and adhesion in the present of sub-MICs (1/2, 1/4 and 1/8 MIC) of essential oils were determined in mucoid <i>P. aeruginosa </i>8821M and compared with controls.</p> <p style="text-align: justify;"><strong>Results:</strong> The MICs of essential oils against <i>P. aeruginosa</i> for<i>&nbsp;M. spicata </i>and<i> C. cyminum</i> oils were obtained 16 and 32 &mu;g/ml respectively. The results show that all oils at 1/2 and 1/4 MICs were significantly reduced all tested virulence factors. At 1/8 MICs, M. spicata had effect just on adhesion but <i><i>C. cyminum </i></i>had effect on&nbsp;Alginate production, biofilm formation, swimming and twitching .</p> <div style="text-align: justify;"><strong>Conclusion:</strong><i> T</i>his study showed that sub-MIC levels of <i><i>M. spicata </i></i>and <i>C. cyminum </i>essential oils affected alginate production, biofilm formation, swimming, twitching and adhesion in<i> <i>P. aeruginosa</i> </i>8821M and it is probable to use of these medicinal plants for treating.</div> Alginate, Biofilm, <,i>,Cumminum cyminum<,/i>,, <,i>,Mentha spicata<,/i>,, <,i>,Pseudomonas aeruginosa<,/i>, Alginate, Biofilm, <,i>,Cumminum cyminum<,/i>,, <,i>,Mentha spicata<,/i>,, <,i>,Pseudomonas aeruginosa<,/i>, 124 130 http://jmp.ir/browse.php?a_code=A-10-414-1&slc_lang=en&sid=1 S Mousavi Nadoshan موسوی ندوشن 100319475328460038156 100319475328460038156 No Department of Biology, Shahed University, Tehran Department of Biology, Shahed University, Tehran P Owlia پرویز اولیا powlia@gmail.com 100319475328460038157 100319475328460038157 Yes Department of Microbiology, Faculty of Medicine, Shahed University, Tehran Department of Microbiology, Faculty of Medicine, Shahed University, Tehran L Moein Najafabadi معین نجف آبادی 100319475328460038158 100319475328460038158 No Department of Biology, Shahed University, Tehran Department of Biology, Shahed University, Tehran I Rasooli رسولی 100319475328460038159 100319475328460038159 No Department of Biology, Shahed University, Tehran Department of Biology, Shahed University, Tehran H Saderi صدری 100319475328460038160 100319475328460038160 No Department of Microbiology, Faculty of Medicine, Shahed University, Tehran Department of Microbiology, Faculty of Medicine, Shahed University, Tehran MH Salari سالاری 100319475328460038161 100319475328460038161 No Department of Microbiology, Faculty of Medicine, Shahed University, Tehran Department of Microbiology, Faculty of Medicine, Shahed University, Tehran