en Phytochemical Variations in Lemon Verbena (<i>Lippia citriodora</i> H.B.K.) Plantlets Affected by Propagation Methods and Soil Type فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Lemon verbena (<em>Lippia citriodora</em> H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in North region of Iran. <strong>Objective:</strong> Evaluation of phytochemical characters in <em>Lippia citriodora</em> H.B.K. plantlets affected by propagation methods (micro-propagation and stem cutting) cultivated in different soil type (peat moss and mixture soil). <strong>Methods:</strong> This study was done on the base of factorial experiment in randomized complete block design with three replications and four treatments. The treatments were propagation methods (micro-propagation and stem cutting) and soil type (peat moss and mixture soil). The volatile oil was extracted by hydro-distillation using clevenger-type apparatus and analyzed by GC and GC/MS. <strong>Results:</strong> The results indicated that the interaction effects of different soils and plantlets types had significant effect (p<0.05) on all studied parameters. The highest content of essential oil (0.79%), geranial (53.52%), neral (31.82%), limonene (11.29%), leaf dry weight (1.43 g) and SPAD value (20.85) were observed in treatment of MPP (micro-propagation plantlets cultivated in peat moss) and the lowest of that was obtained in treatment of SCM (stem cutting plantlets cultivated in mixture soil). <strong>Conclusion:</strong> The peat moss was the best bed in respect of phytochemicals and morpho-physiological traits in both types of in vitro and stem cutting propagation. The highest essential oil content was observed in treatment of micro-propagation cultivated in peat moss.</div> <div dir="ltr" style="text-align: justify;"><strong>Background:</strong> Lemon verbena (<em>Lippia citriodora</em> H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in North region of Iran. <strong>Objective:</strong> Evaluation of phytochemical characters in <em>Lippia citriodora</em> H.B.K. plantlets affected by propagation methods (micro-propagation and stem cutting) cultivated in different soil type (peat moss and mixture soil). <strong>Methods:</strong> This study was done on the base of factorial experiment in randomized complete block design with three replications and four treatments. The treatments were propagation methods (micro-propagation and stem cutting) and soil type (peat moss and mixture soil). The volatile oil was extracted by hydro-distillation using clevenger-type apparatus and analyzed by GC and GC/MS. <strong>Results:</strong> The results indicated that the interaction effects of different soils and plantlets types had significant effect (p<0.05) on all studied parameters. The highest content of essential oil (0.79%), geranial (53.52%), neral (31.82%), limonene (11.29%), leaf dry weight (1.43 g) and SPAD value (20.85) were observed in treatment of MPP (micro-propagation plantlets cultivated in peat moss) and the lowest of that was obtained in treatment of SCM (stem cutting plantlets cultivated in mixture soil). <strong>Conclusion:</strong> The peat moss was the best bed in respect of phytochemicals and morpho-physiological traits in both types of in vitro and stem cutting propagation. The highest essential oil content was observed in treatment of micro-propagation cultivated in peat moss.</div> ,Lippia citriodora H.B.K., Micro-propagation, Peat moss, Soil type, Stem cutting Lippia citriodora H.B.K., Micro-propagation, Peat moss, Soil type, Stem cutting 54 68 http://jmp.ir/browse.php?a_code=A-10-44-1&slc_lang=en&sid=1 M Moradi M Moradi 100319475328460041941 100319475328460041941 No Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran A Mehrafarin A Mehrafarin 100319475328460041942 100319475328460041942 No Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran H Naghdi Badi H Naghdi Badi Naghdibadi@yahoo.com 100319475328460041943 100319475328460041943 Yes Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran Cultivation & Development Department of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran