en Study of Hypoglycemic Effect of <i>Juglans regia</i> Leaves and its Mechanism فارماكوگنوزی و فارماسيوتيكس Pharmacognosy & Pharmaceutics پژوهشی Research <p dir="ltr" style="text-align: justify;"><strong>Background:</strong> Hypoglycemic effect of <i>Juglans regia </i>leaves was reported in other previous researches.</p> <p dir="ltr" style="text-align: justify;"><strong>Objective:</strong> To investigate the mechanism of hypoglycemic action of <i>Julans regia </i>Leaves Methanolic Extract (JRLME), we designed the following study.</p> <p dir="ltr" style="text-align: justify;"><strong>Methods:</strong> Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250 (D+JRLMEa), 500 mg/kg (D+JRLMEb), Acarbose at dose 20 mg/kg (D+Ac), vehicle alone (DC) and normal rats treated with vehicle alone (NC). The Postprandial blood glucose level was examined in a short time (after a single dose treatment) and a long time (after three weeks daily treatment) model. After three weeks of treatment, all groups&rsquo; animals were killed to dissect pancreatic and myocardium for RNA extraction and RT-PCR, to assess insulin and glut-4 genes expression, respectively. In addition, other normal rats were killed to extract intestinal brush border membrane for &alpha;-glucosidase solution preparation. The effect of JRLMEa on &alpha;-glucosidase activity was evaluated by an <i>in vitro </i>method, compared to Acarbose as a reference drug.</p> <p dir="ltr" style="text-align: justify;"><strong>Results:</strong> The plant extract had a significant hypoglycemic action in both short and long term models. Decrease in PBG level was the most at 8 hour after treatment and more in D+Ac (53%), D+JRLMEa (40%) and D+JRLMEb (29%) versus DC, respectively. There was also permanent PBG reduction in treated groups in comparison with the DC one during long term period.</p> <div dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> We did not find change in the insulin and glut-4 genes expression. On the other hand, the <i>in vitro </i>assay of &alpha;-glucosidase activity displayed inhibitory action of JRLME, like Acarbose, but less effectively.</div> <p style="text-align: justify;"><strong>Background:</strong> Hypoglycemic effect of <i>Juglans regia </i>leaves was reported in other previous researches.</p> <p style="text-align: justify;"><strong>Objective:</strong> To investigate the mechanism of hypoglycemic action of <i>Julans regia </i>Leaves Methanolic Extract (JRLME), we designed the following study.</p> <p style="text-align: justify;"><strong>Methods:</strong> Male wistar rats were classified in five groups, each of six, including alloxan-induced diabetic rats treated with JRLME at doses of 250 (D+JRLMEa), 500 mg/kg (D+JRLMEb), Acarbose at dose 20 mg/kg (D+Ac), vehicle alone (DC) and normal rats treated with vehicle alone (NC). The Postprandial blood glucose level was examined in a short time (after a single dose treatment) and a long time (after three weeks daily treatment) model. After three weeks of treatment, all groups&rsquo; animals were killed to dissect pancreatic and myocardium for RNA extraction and RT-PCR, to assess insulin and glut-4 genes expression, respectively. In addition, other normal rats were killed to extract intestinal brush border membrane for &alpha;-glucosidase solution preparation. The effect of JRLMEa on &alpha;-glucosidase activity was evaluated by an <i>in vitro </i>method, compared to Acarbose as a reference drug.</p> <p style="text-align: justify;"><strong>Results:</strong> The plant extract had a significant hypoglycemic action in both short and long term models. Decrease in PBG level was the most at 8 hour after treatment and more in D+Ac (53%), D+JRLMEa (40%) and D+JRLMEb (29%) versus DC, respectively. There was also permanent PBG reduction in treated groups in comparison with the DC one during long term period.</p> <div style="text-align: justify;"><strong>Conclusion:</strong> We did not find change in the insulin and glut-4 genes expression. On the other hand, the <i>in vitro </i>assay of &alpha;-glucosidase activity displayed inhibitory action of JRLME, like Acarbose, but less effectively.</div> <,i>,Juglans regia<,/i>,, Blood Glucose, insulin, glut-4, α-Glucosidase <,i>,Juglans regia<,/i>,, Blood Glucose, insulin, glut-4, α-Glucosidase 57 65 http://jmp.ir/browse.php?a_code=A-10-218-2&slc_lang=en&sid=1 M Teimori مریم تیموری 100319475328460038113 100319475328460038113 No Department of Cellular and Molecular Biology, School of Biology, Faculty of Science, University of Tehran Department of Cellular and Molecular Biology, School of Biology, Faculty of Science, University of Tehran S Montasser Kouhsari منتسر کوهساری 100319475328460038114 100319475328460038114 No Department of Cellular and Molecular Biology, School of Biology, Faculty of Science, University of Tehran Department of Cellular and Molecular Biology, School of Biology, Faculty of Science, University of Tehran R Ghafarzadegan رضا غفارزادگان reza.ghafary@yahoo.com 100319475328460038115 100319475328460038115 Yes Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj R Hajiaghaee رضا حاجی آقایی 100319475328460038116 100319475328460038116 No Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj Department of Pharmacognosy and Pharmaceutics, Institute of Medicinal plants, ACECR, Karaj